Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
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TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
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Detection of endocrine disrupting chemicals in Danio rerio and Daphnia pulex: Step-one, behavioral screen
Lakshmi Neha Reddy Alla,Manahil Monshi,Zoha Siddiqua,Jeremiah N. Shields,Karim Alame,Andrea Wahls,Camille Akemann,Danielle N. Meyer,Emily J. Crofts,Fadie Saad,Judy El-Nachef,Merna Antoon,Raquel Nakhle,Nemer Hijazi,Maha Hamid,Katherine Gurdziel,Shawn P. McElmurry,Donna R. Kashian,Tracie R. Baker,David K. Pitts +19 more
TL;DR: In this article, a bioassay for detecting estrogenic and/or anti-androgenic activity with the goal to evaluate complex mixtures of uncharacterized contaminants in water samples is presented.
Journal ArticleDOI
Comparison of High Performance Liquid Chromatography Methods with Different Detectors for Determination of Steroid Hormones in Aqueous Matrices
TL;DR: In this article, the authors presented the development and comparison of procedures for the qualitative and quantitative determination of five estrogenic compounds (17-α-ethynylestradiol, estrone, estradiol and estriol, and progesterone) in drinking water and wastewater samples.
Journal ArticleDOI
RIFM fragrance ingredient safety assessment, isoeugenol, CAS Registry Number 97-54-1
A.M. Api,D. Belsito,S.P. Bhatia,Magnus Bruze,Peter Calow,Maria Lúcia Zaidan Dagli,W. Dekant,Allison D. Fryer,L Kromidas,S. La Cava,J F Lalko,A. Lapczynski,D. C. Liebler,Yoshiki Miyachi,V. T. Politano,G. Ritacco,D. Salvito,T. W. Schultz,J. Shen,I. G. Sipes,B Wall,D K Wilcox +21 more
TL;DR: This material was evaluated for genotoxicity, repeated dose toxicity, developmental toxicity, reproductive toxicity, local respiratory toxicity, photot toxicity, skin sensitization potential, as well as, environmental safety.
Journal ArticleDOI
Inter- and intra-individual variation in urinary concentrations of parabens in male and female Japanese subjects
Yukiko Nishihama,Reika Ameda,Jun Yoshinaga,Shoko Konishi,Miyuki Yoneyama,Daisuke Nakajima,Hiroaki Shiraishi,Hideki Imai +7 more
TL;DR: The results suggested that four parabens concentrations in a spot urine sample moderately reflected long-term paraben exposure of Japanese subjects.
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A comparative QSAR study on the estrogenic activities of persistent organic pollutants by PLS and SVM
Fei Li,Jialin Liu,Lulu Cao +2 more
TL;DR: The predicted values by the final QSAR models were in good agreement with the corresponding experimental values, and these predictive models should be useful to rapidly identify potential estrogenic endocrine disrupting chemicals.
References
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.