Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
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TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
Citations
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Journal ArticleDOI
Effect-directed analysis of Pimpinella saxifraga L. root extract via HPTLC–UV/Vis/FLD–EDA–MS
Gertrud E. Morlock,Tatjana Lapin +1 more
TL;DR: In this paper, a method was developed for effect-directed analysis (EDA) of the root extract of Pimpinella saxifraga L. root extract, which directly pointed to multi-potent compounds in the root extracts.
Toxic Mechanisms of Pyrethroids Studied in Vitro
TL;DR: This work evaluated the toxicities of pyrethroid compounds in neural (SHSY5Y) and non-neural (MCF-7) cell lines (III, IV) by evaluating the hormone-like effects and in vitro cell culture methods in the evaluation of the toxic effects of pyrethroids.
Dissertation
Oxidation of Bisphenol A, Triclosan and 4-Nonylphenol by Fe-B* activated peroxide
TL;DR: In this article, a systematic investigation of BPA, triclosan and nonylphenol (NP) oxidation by a green catalyst system using Fe-TAML/H2O2 was performed.
Journal ArticleDOI
17β-Estradiol Primes Elicitation of Inducible Nitric Oxide Synthase Expression by Lipopolysaccharide and Interferon-γ in Mouse Macrophage Cell Line J774.1
TL;DR: Results suggest that ERs may participate only partially in iNOS mRNA transcription in J774.1 cells and that 17β-estradiol may act directly through other unknown intracellular signal transduction(s) that are activated by LPS and IFN-γ.
References
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.