Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
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TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
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Effect of various reagents on the decomposition of endocrine disruptors by ozonation: A detailed study
TL;DR: In this paper, the authors investigated the decomposition of two estrogenic hormones progesterone (P4) and 17β estradiol (E2) and a hormone-mimic bisphenol A (BPA) by ozonation and revealed the catalytic role of phosphate ions in triggering ozone side reactions with interfering oxidants, reductants, and organic components.
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Evaluation of estrogenic activity and measurement of EDCs in wastewater treatment plants
TL;DR: In this paper, the relationship between estrogenic activity and DOC/UV260 ratio in wastewater treatment processes was investigated to propose a simple, reliable and comprehensive indicator for the presence of estrogenic substances.
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Exposure of childbearing-aged female to phthalates through the use of personal care products in China: An assessment of absorption via dermal and its risk characterization
TL;DR: Zhang et al. as discussed by the authors derived ERα, ERβ binding, and AR anti-androgenic effects of PAE exposure on female reproduction, according to the daily exposure method and adverse cumulative effects.
Journal ArticleDOI
Teaching the Basics of Nuclear Receptor Action: A Simple Laboratory Exercise Using the Yeast Two-Hybrid Method
TL;DR: A simple hands-on exercise that demonstrated ligand binding, dose responses, and consequences of ligandbinding that are critical for NR function is developed using the yeast two-hybrid method.
Journal ArticleDOI
Development of a New Decision Tree to Rapidly Screen Chemical Estrogenic Activities of Xenopus laevis
TL;DR: The new decision tree can be used to screen the estrogenic activities of the chemicals, and combinational use of the Ebinding and classical physicochemical parameters can greatly improves Hong’s decision tree.
References
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.