Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
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TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
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Journal ArticleDOI
RIFM fragrance ingredient safety assessment, p-isopropylbenzyl alcohol, CAS Registry Number 536-60-7
A.M. Api,D. Belsito,S. Biserta,D. Botelho,Magnus Bruze,G. A. Burton,J. Buschmann,M. A. Cancellieri,Maria Lúcia Zaidan Dagli,M. Date,W. Dekant,C. Deodhar,Allison D. Fryer,S. Gadhia,L. Jones,K. Joshi,A. Lapczynski,M. Lavelle,D. C. Liebler,M. Na,D. O'Brien,A. Patel,T. M. Penning,G. Ritacco,F. Rodriguez-Ropero,J. Romine,N. Sadekar,D. Salvito,Terry W Schultz,F. Siddiqi,I. G. Sipes,G. Sullivan,Y. Thakkar,Yoshiki Tokura,S. Tsang +34 more
TL;DR: P-isopropylbenzyl alcohol was evaluated for genotoxicity, repeated dose toxicity, developmental and reproductive toxicity, local respiratory toxicity, phototoxicity/photoallergenicity, skin sensitization, and environmental safety.
Journal ArticleDOI
Molecular Docking Studies of Glycyrrhizic acid (GA), Glycyrrhetic acid (GE) and Glabridin (GLA) With Estrogen Receptors (Ers)
D. L. Oh,Adeline Yoke Yin Chia +1 more
TL;DR: GLA, not GA and GE, is a partial ERs agonist and could be further modified to design novel semi-synthetic post-menopausal drugs.
Journal ArticleDOI
Determination of Nonylphenol and Octylphenol in Fish and Shellfish by High-Performance Liquid Chromatography/Electrospray Mass Spectrometry
TL;DR: In this article, the authors discuss the effect of selected ion monitoring (SIM) on the performance of 4-NP and 4-OP in terms of the following categories: 1.1）を用いた選択イオン検出（selected ion monitoring.
Book ChapterDOI
Predicting Protein-Protein Interactions from Protein Sequence Information Using Dual-Tree Complex Wavelet Transform
TL;DR: Wang et al. as mentioned in this paper developed a novel computational method for predicting protein-protein interactions by combining dual-tree complex wavelet transform (DTCWT) on substitution matrix representation (SMR) and weighted sparse representation-based classifier (WSRC).
Journal Article
Perinatal Exposure to Low-dose Nonylphenol Specifically Improves Spatial Learning and Memory in Male Rat Offspring.
TL;DR: Perinatal exposure to low-dose NP specifically improves spatial learning and memory in male rat offspring, and in other behavioral tests, NP did not affect any of each behavioral parameter.
References
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.