Estudo do ciclo evolutivo do "Schizotrypanum Cruzi" em cultura de tecidos de embrião de galinha
C. Romaña,H. Meyer +1 more
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TLDR
The authors were able to follow the complete cycle of the parasite, observing its classic evolution: transformation into leishmania, multiplication by binary division, transformation into crithidia and finally into trypanosome.Abstract:
Chick embryo tissue cultures were smeared with Schizotrypanum from different sources. The cultures were inoculated with flagellates from blood-agar cultures and in one instance from blood of an infected guinea-pig. Carrels technique of tissue culture, with slight modifications, was employed. The tissue used were spleen, myocardium, liver, epithelium of the iris, spinal ganglion and monocytes from chicken blood. In all these tissues the flagellate developed easily, parasitizing different types of cells: fibroblasts, histiocytes, macrophages, epithelial cells, cells of the nervous system, etc. The authors were able to follow the complete cycle of the parasite, observing its classic evolution: transformation into leishmania, multiplication by binary division, transformation into crithidia and finally into trypanosome. They also observed forms of the parasite which possibly developed directly from leishmania to trypanosome without apparently going through the crithidia stage. They succeeded in infecting a white mouse with a human strain of S. cruzi after passage through tissue culture. The authors also pointed out different phenomena observed in the relations between the cells and the parasites.read more
Citations
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Journal ArticleDOI
Cultivation of Mammalian Trypanosomes
TL;DR: Development of chemically defined media and of media which will support the growth of trypanosome stages which thus far have not been cultivated in vitro are major problems for the future.
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The tumoricidal effect of Trypanosoma cruzi: its intracellular cycle and the immune response of the host.
TL;DR: It is proposed that the liberation of many endocellular antigens by destruction of some cancer cells, infected with T. cruzi, gives rise to an autoimmune response against antigen of analogouscancer cells, which limits or inhibits tumor growth.
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Trypanosoma cruzi: Development in tissue culture
TL;DR: Comparison of forms obtained by the tissue culture experiments with blood forms found in early parasitemia of infected mice suggests that the same process occurs both in tissue cultures and in the vertebrate, and explains why the fast-moving slender forms are the first to appear in the peripheral blood.
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Nucleotide and polynucleotide synthesis in Trypanosoma cruzi. III. Effect of the aminonucleoside of Stylomycin on the parasite in tissue culture
TL;DR: The effect of the aminonucleoside of Stylomycin on T. cruzi in tissue culture could not be counteracted by adenine, adenosine, desoxyadenosine or the metabolites possibly involved in the de novo synthesis pathway of purine nucleotides.
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Active penetration of Trypanosoma cruzi into host cells: historical considerations and current concepts.
TL;DR: It is concluded that endocytosis, with its many variations, is the only mechanism used by T. cruzi to invade host cells.
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