Ethanol Production from Waste Potato Mash by Using Saccharomyces Cerevisiae
01 Jan 2010-pp 1
TL;DR: In this article, waste potato mash was chosen as a carbon source; however, a pretreatment process is needed to convert starch of potato to fermentable carbon sources through liquefaction and saccharification process.
Abstract: Ethanol is one of the bio-energy sources with high efficiency and low environmental impact. Various raw materials have been using as carbon sources for ethanol production. In this study, waste potato mash was chosen as a carbon source; however, a pretreatment process is needed to convert starch of potato to fermentable carbon sources through liquefaction and saccharification process. Then, the effect of pH, inoculum size and various nitrogen sources to obtain maximum ethanol from waste potato mash was studied. The maximum ethanol concentration and production rates were 27.7 g/L and 5.47 g/L/h, respectively, at controlled pH 5.5, whereas 22.75 g/L and 2.22 g/L/h were obtained at uncontrolled pH. Optimum inoculum size was determined as 3% for maximum ethanol concentration and production rate. Furthermore, five different nitrogen sources (yeast extract, poultry meal, hull and fines mix, feather meal, and meat and bone meal) were evaluated to determine an economical alternative nitrogen source to yeast extract. In conclusion, this study demonstrated the potential for utilization of potato waste for ethanol production.
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TL;DR: In this paper, a comprehensive review of the characteristics of food processing waste for micro-and macro-nutrient composition, and utilization of these materials in alcohol production was provided, and the feasibility of producing alcohols, mainly ethanol and butanol, was investigated.
Abstract: Food processing operations produce large amounts of waste that are rich in nutrients, and although these wastes are utilized to produce value-added products to some extent, the majority of the waste is discarded. This paper provides a comprehensive review of the characteristics of food processing waste for micro- and macro-nutrient composition, and utilization of these materials in alcohol production. The feasibility of producing alcohols, mainly ethanol and butanol, was investigated while identifying the research gaps and suggesting future directions for food processing waste utilization. Ethanol and 1-butanol are the most studied alcohols produced by fermentation of food processing wastes. Methanol is used to a much lesser extent as fuel and produced using chemical conversion methods. Propanol and isobutanol from fermentation of food processing waste are gaining interest more recently, and there are fewer published articles on these products. Alcohols have high market demand as fuels and industrial solvents. Effective utilization of food processing wastes in alcohol production can significantly affect the production economics by not having a need to grow crops for raw materials or acquiring biomass at a high cost. Although theoretically alcohol production from food processing waste appears to be feasible, the technology still has to overcome several constraints.
68 citations
TL;DR: Simultaneous saccharification and fermentation (SSF) exhibited higher bioethanol productivity toward separation hydrolysis and fermentation (SHF) and 5% of Kluyveromyces marxianus K21 inoculum at 40°C resulting in the maximum ethanol concentration and productivity.
64 citations
TL;DR: This study demonstrates the potential utilization of potato powder for ethanol production by using Kufri Bahar flour as a carbon source and finding peptone at 1.5 g/l was found to be best out of the three nitrogen sources tested.
Abstract: Ethanol is one of the bio-energy sources with high efficiency and low environmental impact. Various raw materials have been used as carbon source for ethanol production. In the present study, one varieties of potato that is, Kufri Bahar (KB) flour was chosen as a carbon source. In order to obtain maximum conversion of starch into fermentable sugar, optimum parameters for the liquefaction were determined as 104 to 105°C, 0.15% v/w of α-amylase enzyme solution (300 U/ml) and 30 g dry-weight potato mash/100 ml distilled water, respectively with a 68.86% loss in dry weight during the process. For saccharification process, the optimum dose of amyloglucosidase was 0.35% w/v (300 U/ml) with 16.95% glucose production at pH 5.0 and temperature 60°C after 1 h. The maximum ethanol concentration 7.89% (v/v) was obtained with 10% inoculum size at pH 6.0 after 48 h. Furthermore, out of the three nitrogen (yeast extract, peptone and ammonium sulphate) sources tested for ethanol production, peptone at 1.5 g/l was found to be best (7.58%). In conclusion, this study demonstrates the potential utilization of potato powder for ethanol production.
Key words: Potato starch, bioethanol, liquefaction, saccharification, Saccharomyces cerevisiae MTCC-170.
48 citations
Cites result from "Ethanol Production from Waste Potat..."
...In comparison of these results, Izmirlioglu and Demirci (2012) showed that 3% inoculum size was optimum for maximum ethanol concentration and production rate....
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TL;DR: Alternative sources of fermentable sugars, particularly from lignocellulosic sources, have been extensively investigated and specific topics discussed include the organisms used for fermentation, strategies, such as co-culturing and cell immobilization, used to improved the fermentation process, and the use of genetic engineering to improve the performance of ethanol producing fermenters.
Abstract: The widespread use of corn starch and sugarcane as sources of sugar for the production of ethanol via fermentation may negatively impact the use of farmland for production of food. Thus, alternative sources of fermentable sugars, particularly from lignocellulosic sources, have been extensively investigated. Another source of fermentable sugars with substantial potential for ethanol production is the waste from the food growing and processing industry. Reviewed here is the use of waste from potato processing, molasses from processing of sugar beets into sugar, whey from cheese production, byproducts of rice and coffee bean processing, and other food processing wastes as sugar sources for fermentation to ethanol. Specific topics discussed include the organisms used for fermentation, strategies, such as co-culturing and cell immobilization, used to improve the fermentation process, and the use of genetic engineering to improve the performance of ethanol producing fermenters.
36 citations
Cites background from "Ethanol Production from Waste Potat..."
...Food processing wastes such as molasses [5, 30, 59], whey, or potato wastes [2, 5, 96, 110] have shown to be potential sources for ethanol production....
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...Potato peel, mash Starch, pectin, cellulose, and hemicellulose [10, 95, 96, 216]...
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...In a study conducted by Izmirlioglu and Demirci [96], hydrolyzing conditions for solid waste potato mash from manufacturing of potato flakes were optimized and the resulting medium used for fermentation to ethanol (about 31 g/L) by Saccharomyces cerevisiae....
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TL;DR: Medium optimization for glucoamylase activity and glucose production were improved 126% and 98% compared to only industrial waste potato mash basal medium and the results showed that the optimal medium composition for Aspergillus niger van Tieghem was 50 g L(-1) industrial waste Potato mash supplemented with 51.82 g L (-1) malt extract.
Abstract: Background Glucoamylase is one of the most common enzymes used in the food industry to break down starch into its monomers. Glucoamylase production and its activity are highly dependent on medium composition. Starch is well known as a glucoamylase inducer, and utilization of industrial starchy potato waste is an inexpensive way of improving glucoamylase production. Since glucoamylase production is highly dependent on medium composition, in this study medium optimization for glucoamylase production was considered to enhance glucoamylase activity. Results Among the evaluated microbial species, Aspergillus niger van Tieghem was found to be the best glucoamylase-producing fungus. The Plackett-Burman design was used to screen various medium ingredients, and malt extract, FeSO4 .7H2 O and CaCl2 ·2H2 O were found to have significant effects on glucoamylase production. Finally, malt extract, FeSO4 .7H2 O and CaCl2 .2H2 O were optimized by using a central composite design of response surface methodology. The results showed that the optimal medium composition for A. niger van Tieghem was 50 g L(-1) industrial waste potato mash supplemented with 51.82 g L(-1) malt extract, 9.27 g L(-1) CaCl2 ·2H2 O and 0.50 g L(-1) FeSO4 .7H2 O. Conclusion At the end of optimization, glucoamylase activity and glucose production were improved 126% and 98% compared to only industrial waste potato mash basal medium; 274.4 U mL(-1) glucoamylase activity and 41.7 g L(-1) glucose levels were achieved, respectively. © 2015 Society of Chemical Industry.
26 citations
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"Ethanol Production from Waste Potat..." refers background in this paper
...Hydrolysis is carried out at high temperature (90–110 °C); however, at low temperatures, it is possible and can contribute to energy savings [10]....
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"Ethanol Production from Waste Potat..." refers background in this paper
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TL;DR: PPW, a by-product of the potato industry features a high potential for ethanol production, and is hydrolyzed with various enzymes and/or acid, and fermented by Saccharomyces cerevisae var.
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