Eukaryotic Viruses in Wastewater Samples from the United States
Citations
372 citations
Cites background or methods from "Eukaryotic Viruses in Wastewater Sa..."
...Thesemethods have been previously used to concentrate viruses from (waste)water (Symonds et al., 2009; Ikner et al., 2011) and recently used for SARS-CoV-2 RNA detection in untreated wastewater (Ahmed et al....
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...This centrifugal concentration step was repeated three times to pass through the entire supernatant volume (Symonds et al., 2009; Ikner et al., 2011; Ahmed et al., 2015)....
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...Methods D and E were ultrafiltration methods that use centrifugal devices that have previously been used to concentrate viruses from (waste)water (Symonds et al., 2009; Ikner et al., 2011)....
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277 citations
253 citations
Cites background from "Eukaryotic Viruses in Wastewater Sa..."
...Wastewater samples were collected from 12 treatment facilities in 11 coastal states from the United States to determine the prevalence of PMMoV....
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...This study analyzed the presence of PMMoV in raw sewage and treated wastewater samples collected from wastewater treatment facilities throughout the coastal United States....
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...Here we demonstrate that the plant pathogen Pepper mild mottle virus (PMMoV) is widespread and abundant in wastewater from the United States, suggesting the utility of this virus as an indicator of human fecal pollution....
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...Ten-milliliter samples of raw sewage and treated wastewater were gathered from 12 wastewater treatment facilities throughout the coastal United States (Table 1) and were concentrated according to the methods of Symonds et al. (41)....
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...Picobirnaviruses and Torque teno virus are abundant in raw sewage from some regions and thus have also been proposed as indicator viruses (15, 41)....
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248 citations
192 citations
Cites background from "Eukaryotic Viruses in Wastewater Sa..."
...The selection of the hPBV for the present study was due to its high prevalence in wastewater samples (Symonds et al., 2009)....
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References
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"Eukaryotic Viruses in Wastewater Sa..." refers methods in this paper
...8 (73), and phylogenetic analyses were carried out with MEGA v....
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3,924 citations
"Eukaryotic Viruses in Wastewater Sa..." refers methods in this paper
...In addition to dereplicating sequences, FastGroup II (81) was also used to calculate the Chao1 value, a richness estimator (22, 23), and to plot a rarefaction curve, which displays the number of unique sequences versus the number of clones sequenced (42, 46)....
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