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Journal ArticleDOI

Evaluation of 4-methyl-2-[(2-methylbenzyl) amino]-1,3-thiazole-5-carboxylic acid against hyperglycemia, insulin sensitivity, and oxidative stress-induced inflammatory responses and β-cell damage in the pancreas of streptozotocin-induced diabetic rats.

01 Feb 2018-Human & Experimental Toxicology (SAGE PublicationsSage UK: London, England)-Vol. 37, Iss: 2, pp 163-174
TL;DR: The results suggest that BAC successfully reduces the blood glucose level and possesses antioxidant as well as anti-inflammatory activities, which leads to decreased histological damage in diabetic pancreatic tissues, suggesting the possibility of future diabetes treatments.
Abstract: 4-Methyl-2-[(2-methylbenzyl) amino]-1,3-thiazole-5-carboxylic acid (bioactive compound (BAC)), a novel thiazole derivative, is a xanthine oxidase inhibitor and free radical scavenging agent. Effects of BAC on hyperglycemia, insulin sensitivity, oxidative stress, and inflammatory mediators were evaluated in streptozotocin (STZ)-induced neonatal models of non-insulin-dependent diabetes mellitus (NIDDM) rats where NIDDM was induced in neonatal pups with single intraperitoneal injection of STZ (100 mg/kg). The effect of BAC (10 and 20 mg/kg, p.o.) for 3 weeks was evaluated by the determination of blood glucose, oral glucose tolerance test (OGTT), HbA1c level, insulin level, insulin sensitivity, and insulin resistance (IR). Furthermore, inflammatory mediators (tumor necrosis factor-alpha and interleukin-6) and oxidative stress were estimated in serum and pancreatic tissue, respectively. Significant alteration in the level of blood glucose, OGTT, HbA1c, insulin level, insulin sensitivity, in addition variation in the antioxidant status and inflammatory mediators, and alteration in histoarchitecture of pancreatic tissue confirmed the potential of BAC in STZ-induced neonatal models of NIDDM rats. Pretreatment with BAC restored the level of glucose by decreasing the IR and increasing the insulin sensitivity. Furthermore, BAC balanced the antioxidant status and preserved the inflammatory mediators. Histological studies of pancreatic tissues showed normal architecture after BAC administration to diabetic rats. Altogether, our results suggest that BAC successfully reduces the blood glucose level and possesses antioxidant as well as anti-inflammatory activities. This leads to decreased histological damage in diabetic pancreatic tissues, suggesting the possibility of future diabetes treatments.
Citations
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TL;DR: NDTD is suggested as potential therapeutic candidate for T2DM and histopathological study of liver suggests the protective role of NDTD against hepatic injury, as diabetic rats exposed with NDTD shows significantly reduction in inflammation and lesion as compared to STZ control rats.

4 citations

Journal ArticleDOI
TL;DR: In this paper , the interaction of TZL with pure and hydroxyl-functionalized boron nitride (OH-B12N12) nanoclusters was assessed using density functional theory (DFT) and molecular docking calculations.
References
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Journal Article
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.

289,852 citations


"Evaluation of 4-methyl-2-[(2-methyl..." refers methods in this paper

  • ...Supernatant of homogenates was used to estimate LPO (malondialdehyde (MDA)) according to the method of Ohkawa et al.,26 reduced GSH by Moron et al.,27 superoxide dismutase (SOD) activity by Misra and Fridovich,28 and CAT activity by Sinha.29 Protein concentration was determined using BSA as standard by Lowry et al.30 Estimation of TNF-a and IL-6 The quantifications of TNF-a and IL-6 were performed in the serum according to instructions provided by the rat TNF-a and IL-6 ELISA kit manufacturer eBioscience....

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  • ...Protein concentration was determined using BSA as standard by Lowry et al.(30)...

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Journal ArticleDOI
TL;DR: Using this method, the liped peroxide level in the liver of rats suffering from carbon tetrachloride intoxication was investigated and was in good agreement with previously reported data obtained by measuring diene content.

24,847 citations

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TL;DR: It is proposed that the autoxidation of epinephrine proceeds by at least two distinct pathways, only one of which is a free radical chain reaction involving O2- and hence inhibitable by superoxide dismutase.

7,872 citations

Journal ArticleDOI
Asru K. Sinha1
TL;DR: A simple colorimetric assay for catalase activity has been described using K2Cr2O7/acetic acid reagent to determine values of different enzyme sources and compared with the values obtained by titrimetric methods.

4,827 citations


"Evaluation of 4-methyl-2-[(2-methyl..." refers methods in this paper

  • ...,(27) superoxide dismutase (SOD) activity by Misra and Fridovich,(28) and CAT activity by Sinha.(29) Protein concentration was determined using BSA as standard by Lowry et al....

    [...]

  • ...Supernatant of homogenates was used to estimate LPO (malondialdehyde (MDA)) according to the method of Ohkawa et al.,26 reduced GSH by Moron et al.,27 superoxide dismutase (SOD) activity by Misra and Fridovich,28 and CAT activity by Sinha.29 Protein concentration was determined using BSA as standard by Lowry et al.30 Estimation of TNF-a and IL-6 The quantifications of TNF-a and IL-6 were performed in the serum according to instructions provided by the rat TNF-a and IL-6 ELISA kit manufacturer eBioscience....

    [...]