Evaluation of anti-arthritic, HRBC membrane stabilization and antioxidant properties of the edible Phlogacanthus thyrsiformis (Hardow) Mabb flower extracts and their correlation studies
02 Jan 2017-Vol. 11, Iss: 1, pp 1-7
TL;DR: In this paper, the anti-arthritic and anti-inflammatory properties of P. thyrsiformis flowers were investigated using gas chromatography mass spectrometry technique and eight molecules from the ethyl acetate extract using the NIST library.
Abstract: The present experiments were designed to scientifically prove the anti-arthritic and anti-inflammatory properties of traditionally used P. thyrsiformis flowers. The flower extracts were subjected to qualitative and quantitative screening. Gas chromatography mass spectrometry technique was employed and eight molecules were identified from the ethyl acetate extract using the NIST library. The extracts were also evaluated for in vitro antioxidant, anti-arthritic and anti-inflammatory activities. Potent antioxidant activity was observed for the ethyl acetate extract in both antioxidant assay methods with an IC50 value of 63.36 μg/mL and 64.60 μg/mL, respectively. The ethyl acetate extract also exhibited significant activity in both anti-arthritic and anti-inflammatory activities. Correlation studies revealed that the presence of phenol, flavonoid, terpenoid and s-sitosterol in the ethyl acetate extract played a crucial role in its impressive activity. This study provided experimental evidence towards ...
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TL;DR: The aim of this review is to provide new findings on health effects of edible flowers since 2015, and some newly found phytochemicals such as polysaccharides were shown to be beneficial to human health.
48 citations
TL;DR: In this paper, a review of the chemical composition of various species of edible flowers is presented, and the main groups of compounds that are usually present in the species that are most commonly used for culinary purposes.
Abstract: Edible flowers are becoming very popular, as consumers are seeking healthier and more attractive food products that can improve their diet aesthetics and diversify their dietary sources of micronutrients. The great variety of flowers that can be eaten is also associated with high variability in chemical composition, especially in bioactive compounds content that may significantly contribute to human health. The advanced analytical techniques allowed us to reveal the chemical composition of edible flowers and identify new compounds and effects that were not known until recently. Considering the numerous species of edible flowers, the present review aims to categorize the various species depending on their chemical composition and also to present the main groups of compounds that are usually present in the species that are most commonly used for culinary purposes. Moreover, special attention is given to those species that contain potentially toxic or poisonous compounds as their integration in human diets should be carefully considered. In conclusion, the present review provides useful information regarding the chemical composition and the main groups of chemical compounds that are present in the flowers of the most common species.
9 citations
TL;DR: In this modern world, it is essential to adopt both ex situ and in situ methods as complementary techniques for the conservation of the medicinal plant population as discussed by the authors , and a national policy needs to be framed and implemented to conserve the population of medicinal plant species.
Abstract: Medicinal plants are valuable resources for drugs, and the major population in developing countries depends on herbal drugs. Increasing demands on the use of medicinal plants as primary therapeutics put pressure on the conservation strategies of wild medicinal plants. The north-eastern region is considered a reservoir of many medicinal plants accounting for approximately half of the available medicinal plants in India. Many medicinal plant species are endemic to this region, and many growing in the wild are left unaware, so there is a need for identification and conservation. National policy needs to be framed and implemented to conserve the population of medicinal plant species. In this modern world, it is essential to adopt both ex situ and in situ methods as complementary techniques for the conservation of the medicinal plant population.
References
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TL;DR: An antioxidant fraction of Chinese green tea, containing several catechins, has been previously shown to inhibit 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced tumor promotion in mouse skin and was shown to have antioxidative activity toward hydrogen peroxide and the superoxide radical.
Abstract: An antioxidant fraction of Chinese green tea (green tea antioxidant; GTA), containing several catechins, has been previously shown to inhibit 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced tumor promotion in mouse skin. In the present study, GTA was shown to have antioxidative activity toward hydrogen peroxide (H2O2) and the superoxide radical (O2-). GTA also prevented oxygen radical and H2O2-induced cytotoxicity and inhibition of intercellular communication in cultured B6C3F1 mouse hepatocytes and human keratinocytes (NHEK cells). GTA (0.05-50 micrograms/ml) prevented the killing of hepatocytes (measured by lactate dehydrogenase release) by paraquat (1-10 mM) and glucose oxidase (0.8-40 micrograms/ml) in a concentration-dependent fashion. GTA (50 micrograms/ml) also prevented the inhibition of hepatocyte intercellular communication by paraquat (5 mM), glucose oxidase (0.8 micrograms/ml), and phenobarbital (500 micrograms/ml). In addition, GTA (50 micrograms/ml) prevented the inhibition of intercellular communication in human keratinocytes by TPA (100 ng/ml). Cytotoxicity and inhibition of intercellular communication, two possible mechanisms by which tumor promoters may produce their promoting effects were therefore prevented by GTA. The inhibition of these two effects of pro-oxidant compounds may suggest a mechanism by which GTA inhibits tumor promotion in vivo.
1,895 citations
"Evaluation of anti-arthritic, HRBC ..." refers methods in this paper
...The hydrogen peroxide scavenging activity of the extracts was estimated using the procedure reported by Ruch et al. (1989)....
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TL;DR: The antioxidant properties of Sechium edule extracts were tested by three established in vitro methods, namely reducing power, β-carotene linoleate model and 1,1- diphenyl-2 picrylhydrazyl (DPPH) radical-scavenging.
928 citations
"Evaluation of anti-arthritic, HRBC ..." refers methods in this paper
...Total flavonoid content was determined using the technique reported by Ordonez et al. (2006) and Gursoy et al. (2009)....
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TL;DR: In this article, the authors examined the in vitro radical scavenging and antioxidant capacity of tannic acid by using different in vitro analytical methodologies such as 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH ) scavenging, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) activity, total antioxidant activity determination by ferric thiocyanate, total reducing ability determination using by Fe3+−Fe2+ transformation
715 citations
"Evaluation of anti-arthritic, HRBC ..." refers methods in this paper
...The extracts were evaluated for antioxidant activity by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay (Gulcin et al. 2006; Gulcin et al. 2010)....
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01 Jan 2010
TL;DR: Methanol extract of the whole plant of Oxalis corniculata Linn (Family: Oxalidaceae) was assessed for its antioxidant and anti-inflammatory activity by using in-vitro methods.
Abstract: Methanol extract of whole plant of Oxalis corniculata Linn (Family: Oxalidaceae) was assessed for its antioxidant and anti‐inflammatory activity by invitro methods. Antioxidant activity was studied using 1, 1‐Diphenyl‐2‐Picrylhydrazyl (DPPH) and nitric oxide radical scavenging activity. Inhibition of lipid peroxidation was studied by thiobarbituric acid reactive substances (TBARS) method on isolated rat liver tissues. Quantitative analysis of antioxidative components like total amount of phenolics, flavonoids and flavonols were estimated using spectrophotometric method. Invitro anti‐inflammatory activity was evaluated using albumin denaturation assay, membrane stabilization assay and proteinase inhibitory activity at different concentrations. Aspirin was used as a sta ndard drug for the study of anti‐ inflammatory activity. Linear regression analysis was used to calculate IC50 value. Results showed that, the extract exhibited significant DPPH and nitric oxide radical scavenging activity with IC50 v alue of 302.93±4.17 and 73.07±8.28µg/ml respectively. Lipid peroxidation induced by the Fe2+, was inhibited by the extract with IC50 value 58.71±2.55µg/ml. Total phenol content was estimated as 25.62±0.10mg of gallic acid equivalents of dry extract. Total flavonoids and flavonols were found to be 150.88±12.61 and 150.16±2.16 mg of rutin equivalents per gram of dry extract respectively. Extract also showed invitro anti‐inflammatory activity by inhibiting the heat induced albumin denaturation and Red Blood Cells membrane stabilization with the IC50 values of 288.04±2.78 and 467.14±9.56µg/ml respectively. Proteinase activity was also significantly inhibited by the extract (IC50=435.28±5.82µg/ml). From the results, it is concluded that flavonoids and related polyphenols present in the O. corniculata extract may be responsible for the activity.
383 citations
"Evaluation of anti-arthritic, HRBC ..." refers methods in this paper
...The anti-inflammatory activity of the extracts was tested by HRBC membrane stabilization method (Azeem et al. 2010; Sakat et al. 2010; Leelaprakash and Dass 2011; Kar et al. 2012)....
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TL;DR: In this paper, the antioxidant capacities of the acetone, methanol and water extracts of hot-air dried lychee (Litchi chinenesis sonn.) flowers were estimated with Trolox equivalent antioxidant capacity (TEAC) assay, reducing power and 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) radical-scavenging assay.
241 citations
"Evaluation of anti-arthritic, HRBC ..." refers background in this paper
...The potent antioxidant activity of the extracts can be accredited to the presence of phenols and flavonoids (Liu et al. 2009; Rengasamy et al. 2010)....
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