scispace - formally typeset
Journal ArticleDOI: 10.1080/09735070.2017.1311692

Evaluation of anti-arthritic, HRBC membrane stabilization and antioxidant properties of the edible Phlogacanthus thyrsiformis (Hardow) Mabb flower extracts and their correlation studies

02 Jan 2017-Vol. 11, Iss: 1, pp 1-7
Abstract: The present experiments were designed to scientifically prove the anti-arthritic and anti-inflammatory properties of traditionally used P. thyrsiformis flowers. The flower extracts were subjected to qualitative and quantitative screening. Gas chromatography mass spectrometry technique was employed and eight molecules were identified from the ethyl acetate extract using the NIST library. The extracts were also evaluated for in vitro antioxidant, anti-arthritic and anti-inflammatory activities. Potent antioxidant activity was observed for the ethyl acetate extract in both antioxidant assay methods with an IC50 value of 63.36 μg/mL and 64.60 μg/mL, respectively. The ethyl acetate extract also exhibited significant activity in both anti-arthritic and anti-inflammatory activities. Correlation studies revealed that the presence of phenol, flavonoid, terpenoid and s-sitosterol in the ethyl acetate extract played a crucial role in its impressive activity. This study provided experimental evidence towards ... more

Topics: Ethyl acetate (56%)

Journal ArticleDOI: 10.1016/J.FOODCHEM.2020.127940
Jingyun Zheng1, Baiyi Lu2, Baojun Xu3Institutions (3)
15 Mar 2021-Food Chemistry
Abstract: The aim of this review is to provide new findings on health effects of edible flowers since 2015. The antioxidant, anti-inflammatory, anti-cancer, hepatoprotective, neuroprotective, anti-diabetic, anti-osteoporosis, anti-obesity, and anti-hypertensive have been reviewed, and the effective concentrations of flower extracts have been summarized. Among all the health benefits mentioned, anti-osteoporosis, anti-obesity, and anti-hypertensive have rarely been mentioned before 2015. Some health benefits mechanisms of edible flowers were discussed frequently after 2015. Some newly found phytochemicals such as polysaccharides were shown to be beneficial to human health. Species of Rosa, Chrysanthemum, and Osmanthus have been reported to exert different health effects on human. For the toxicity studies, the safe level of flower extracts in cell and animal models were at hundreds of parts per million (ppm) level. In consideration of health promoting effects and toxicities of edible flowers, they could serve as potential natural health products for different health benefits. more

17 Citations

Open accessJournal ArticleDOI: 10.3390/MOLECULES26226940
17 Nov 2021-Molecules
Abstract: Edible flowers are becoming very popular, as consumers are seeking healthier and more attractive food products that can improve their diet aesthetics and diversify their dietary sources of micronutrients. The great variety of flowers that can be eaten is also associated with high variability in chemical composition, especially in bioactive compounds content that may significantly contribute to human health. The advanced analytical techniques allowed us to reveal the chemical composition of edible flowers and identify new compounds and effects that were not known until recently. Considering the numerous species of edible flowers, the present review aims to categorize the various species depending on their chemical composition and also to present the main groups of compounds that are usually present in the species that are most commonly used for culinary purposes. Moreover, special attention is given to those species that contain potentially toxic or poisonous compounds as their integration in human diets should be carefully considered. In conclusion, the present review provides useful information regarding the chemical composition and the main groups of chemical compounds that are present in the flowers of the most common species. more


Journal ArticleDOI: 10.1093/CARCIN/10.6.1003
01 Jun 1989-Carcinogenesis
Abstract: An antioxidant fraction of Chinese green tea (green tea antioxidant; GTA), containing several catechins, has been previously shown to inhibit 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced tumor promotion in mouse skin. In the present study, GTA was shown to have antioxidative activity toward hydrogen peroxide (H2O2) and the superoxide radical (O2-). GTA also prevented oxygen radical and H2O2-induced cytotoxicity and inhibition of intercellular communication in cultured B6C3F1 mouse hepatocytes and human keratinocytes (NHEK cells). GTA (0.05-50 micrograms/ml) prevented the killing of hepatocytes (measured by lactate dehydrogenase release) by paraquat (1-10 mM) and glucose oxidase (0.8-40 micrograms/ml) in a concentration-dependent fashion. GTA (50 micrograms/ml) also prevented the inhibition of hepatocyte intercellular communication by paraquat (5 mM), glucose oxidase (0.8 micrograms/ml), and phenobarbital (500 micrograms/ml). In addition, GTA (50 micrograms/ml) prevented the inhibition of intercellular communication in human keratinocytes by TPA (100 ng/ml). Cytotoxicity and inhibition of intercellular communication, two possible mechanisms by which tumor promoters may produce their promoting effects were therefore prevented by GTA. The inhibition of these two effects of pro-oxidant compounds may suggest a mechanism by which GTA inhibits tumor promotion in vivo. more

1,715 Citations

Journal ArticleDOI: 10.1016/J.FOODCHEM.2005.05.024
01 Aug 2006-Food Chemistry
Abstract: The antioxidant activities (AA) of Sechium edule extracts were tested by three established in vitro methods, namely reducing power, β-carotene linoleate model and 1,1- diphenyl-2 picrylhydrazyl (DPPH) radical-scavenging. Leaf ethanolic extracts and leaf and seed water extracts showed strong inhibitory activity by β-carotene bleaching (AA values of 90%). Furthermore, these extracts exerted hydrogen-donating ability in the presence of DDPH stable radical (IC 50 2 μg/ml). These extracts also showed strong reducing power by the potassium ferricyanide reduction method. Leaf and seed extracts may be exploited as biopreservatives in food applications as well as for health supplements or functional food, to aleviate oxidative stress. more

Topics: DPPH (53%), Sechium (52%)

817 Citations

Open accessJournal ArticleDOI: 10.1016/J.ARABJC.2009.12.008
Abstract: Tannic acid, a naturally occurring plant polyphenol, is composed of a central glucose molecule derivatized at its hydroxyl groups with one or more galloyl residues. In the present paper, we examines the in vitro radical scavenging and antioxidant capacity of tannic acid by using different in vitro analytical methodologies such as 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH ) scavenging, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, total antioxidant activity determination by ferric thiocyanate, total reducing ability determination using by Fe3+–Fe2+ transformation method, superoxide anion radical scavenging by riboflavin–methionine-illuminate system, hydrogen peroxide scavenging and ferrous ions (Fe2+) chelating activities. Also, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), α-tocopherol and trolox, a water-soluble analogue of tocopherol, were used as the reference antioxidant radical scavenger compounds. Tannic acid inhibited 97.7% lipid peroxidation of linoleic acid emulsion at 15 μg/mL concentration. On the other hand, the above mentioned standard antioxidants indicated an inhibition of 92.2%, 99.6%, 84.6% and 95.6% on peroxidation of linoleic acid emulsion at 45 μg/mL concentration, respectively. In addition, tannic acid had an effective DPPH scavenging, ABTS + radical scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging, Fe3+ reducing power and metal chelating on ferrous ions activities. Also, those various antioxidant activities were compared to BHA, BHT, α-tocopherol and trolox as references antioxidant compounds. The present study shows that tannic acid is the effective natural antioxidant component that can be used as food preservative agents or nutraceuticals. more

Topics: Trolox (60%), Tannic acid (58%), Butylated hydroxyanisole (58%) more

572 Citations

Open access
01 Jan 2010-
Abstract: Methanol extract of whole plant of Oxalis corniculata Linn (Family: Oxalidaceae) was assessed for its antioxidant and anti‐inflammatory activity by in­vitro methods. Antioxidant activity was studied using 1, 1‐Diphenyl‐2‐Picrylhydrazyl (DPPH) and nitric oxide radical scavenging activity. Inhibition of lipid peroxidation was studied by thiobarbituric acid reactive substances (TBARS) method on isolated rat liver tissues. Quantitative analysis of antioxidative components like total amount of phenolics, flavonoids and flavonols were estimated using spectrophotometric method. In­vitro anti‐inflammatory activity was evaluated using albumin denaturation assay, membrane stabilization assay and proteinase inhibitory activity at different concentrations. Aspirin was used as a sta ndard drug for the study of anti‐ inflammatory activity. Linear regression analysis was used to calculate IC50 value. Results showed that, the extract exhibited significant DPPH and nitric oxide radical scavenging activity with IC50 v alue of 302.93±4.17 and 73.07±8.28µg/ml respectively. Lipid peroxidation induced by the Fe2+, was inhibited by the extract with IC50 value 58.71±2.55µg/ml. Total phenol content was estimated as 25.62±0.10mg of gallic acid equivalents of dry extract. Total flavonoids and flavonols were found to be 150.88±12.61 and 150.16±2.16 mg of rutin equivalents per gram of dry extract respectively. Extract also showed in­vitro anti‐inflammatory activity by inhibiting the heat induced albumin denaturation and Red Blood Cells membrane stabilization with the IC50 values of 288.04±2.78 and 467.14±9.56µg/ml respectively. Proteinase activity was also significantly inhibited by the extract (IC50=435.28±5.82µg/ml). From the results, it is concluded that flavonoids and related polyphenols present in the O. corniculata extract may be responsible for the activity. more

Topics: DPPH (54%), TBARS (51%)

316 Citations

Journal ArticleDOI: 10.1016/J.FOODCHEM.2008.09.088
Shih Chuan Liu1, Jau Tien Lin1, Chin Kun Wang1, Hsin Yi Chen1  +1 moreInstitutions (1)
15 May 2009-Food Chemistry
Abstract: The antioxidant capacities of the acetone, methanol and water extracts of hot-air dried lychee ( Litchi chinenesis Sonn.) flowers were estimated with Trolox equivalent antioxidant capacity (TEAC) assay, reducing power and 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) radical-scavenging assay. The contents of antioxidant components in these extracts were also determined. Results showed that the highest and lowest contents of these components including phenols, flavonoids and condensed tannins were found in acetone and water extracts, respectively. The antioxidant activities of the lychee flower extracts for all assays were in the order: acetone extract > methanol extract > water extract. The contents of antioxidant components in these extracts were correlated with antioxidant activities. more

210 Citations