Evaluation of enzyme immunoassay and radioimmunoassay methods for the measurement of plasma oxytocin
Angela Szeto,Philip M. McCabe,Daniel A. Nation,Daniel A. Nation,Benjamin A. Tabak,Maria A. Rossetti,Michael E. McCullough,Neil Schneiderman,Armando J. Mendez +8 more
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TLDR
It is indicated that sample extraction is necessary to obtain valid assay results for plasma oxytocin measurement using a commercially available enzyme immunoassay (EIA) and radioimmunoASSay (RIA) to assess the immunospecificity of the assays.Abstract:
Objective
There is increased interest in measuring peripheral oxytocin levels to better understand the role of this peptide in mammalian behavior, physiology, and disease. The purpose of this study was to compare methods for plasma oxytocin measurement using a commercially available enzyme immunoassay (EIA) and radioimmunoassay (RIA), to evaluate the need for sample extraction, and to assess the immunospecificity of the assays.read more
Citations
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The Oxytocin Receptor: From Intracellular Signaling to Behavior
Benjamin Jurek,Inga D. Neumann +1 more
TL;DR: The mechanisms of OXT expression and release, expression and binding of the OXTR in brain and periphery, OX TR-coupled signaling cascades, and their involvement in behavioral outcomes are discussed to assemble a comprehensive picture of the central and peripheral OXT system.
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Intranasal Oxytocin: Myths and Delusions
Gareth Leng,Mike Ludwig +1 more
TL;DR: Effects of intranasal oxytocin also need proper dose-response studies, and such studies need to include control subjects for peripheral effects, by administering Oxytocin peripherally and by blocking peripheral actions with antagonists.
Journal ArticleDOI
Oxytocin Pathways and the Evolution of Human Behavior
TL;DR: Oxytocin dynamically moderates the autonomic nervous system, and effects of oxytocin on vagal pathways, as well as the antioxidant and anti-inflammatory effects of this peptide, help to explain the pervasive adaptive consequences of social behavior for emotional and physical health.
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Vasopressin and oxytocin receptor systems in the brain: Sex differences and sex-specific regulation of social behavior.
Kelly M. Dumais,Alexa H. Veenema +1 more
TL;DR: This review discusses the evidence showing the presence or absence of sex differences in VP and OT receptors in rodents and humans, as well as showing new data of sexually dimorphic V1a receptor binding in the rat brain.
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Problems with measuring peripheral oxytocin: can the data on oxytocin and human behavior be trusted?
TL;DR: Evaluation of commercially available methods for measuring OT reveals that they lack reliability when used on unextracted samples of human fluids, and that they tag molecules in addition to OT, yielding estimates that are wildly discrepant with an extensive body of earlier findings.
References
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Oxytocin may mediate the benefits of positive social interaction and emotions
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Charlotte Modahl,Lee Anne Green,Deborah Fein,Mariana Morris,Lynn Waterhouse,Carl Feinstein,Harriet Levin +6 more
TL;DR: The data suggest that OT abnormalities may exist in autism, and that more direct investigation of central nervous system OT function is warranted, after making inferences to central OT functioning from peripheral measurement.
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Central oxytocin administration reduces stress-induced corticosterone release and anxiety behavior in rats.
TL;DR: Oxytocin exerts a central anxiolytic-like effect on both endocrine and behavioral systems and could play a role in moderating behavioral and physiological responses to stress.