Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies
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Cites methods from "Evaluation of general 16S ribosomal..."
...Primers used were: S-D-Bact-0564-a-S- 15, 5′AYTGGGYDTAAAGNG and S-D-Bact-0785-b-A-18, 5TACNVGGGTATCTAATCC [65] generating a 253 bp amplicon....
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References
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Additional excerpts
...Primer pairs were: (i): S-D-Bact-0341b-S-17, 50-CCTACGGGNGGCWGCAG-30 (32), and S-D-Bact-0785-a-A-21, 50-GACTACHVGGGTATCTA ATCC-3 (32); and (ii): S-D-Bact-0008-a-S-16, 50-AGAG TTTGATCMTGGC-30 (33), and S-D-Bact-0907-a-A-20, 50-CCGTCAATTCMTTTGAGTTT-30 (34)....
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"Evaluation of general 16S ribosomal..." refers background in this paper
...For example, ‘0338’ stands for start position 338 in the Escherichia coli system of nomenclature (23); (5) A single lowercase letter indicating the version of the probe....
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...In 2006, Roche’s 454 GS 20 pyrosequencing (5) became the first high-throughput sequencing technology to be successfully applied for large scale biodiversity analysis and was key to uncovering the ‘rare biosphere’ (6)....
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6,840 citations
"Evaluation of general 16S ribosomal..." refers background in this paper
...The attractiveness of Illumina (8) lies in the reduced per base costs and comparatively high sequencing depth (9), despite having short read lengths....
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