Evaluation of the Protective Effect of Hibiscus sabdariffa L. Calyx (Malvaceae) Extract on Arsenic Induced Genotoxicity in Mice and Analysis of its Antioxidant Properties
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The results revealed that the calyx extract of Roselle inhibited the DNA damage induced by sodium arsenite in a dose dependent manner and the presence of phytochemical constituents such as polyphenols and flavonoids were ascribed to the observed changes.Abstract:
The present study envisages the antigenotoxic property of Hibiscus sabdariffa L. (Roselle) calyx extracts that is presumably attributed to its antioxidant properties. The dried calyx extracts of Roselle were administered to male albino mice at doses of 50, 100, and 150 mg/kg body weight for 7 days followed by a single dose of interperitoneal (i.p.) injection of sodium arsenite (2.5 mg/kg body weight) and the extent of DNA damage was analysed by widely used Comet assay. The results revealed that the calyx extract of Roselle inhibited the DNA damage induced by sodium arsenite in a dose dependent manner. The presence of phytochemical constituents such as polyphenols and flavonoids were ascribed to the observed changes. The antioxidant efficacy was substantiated by applying Ferric Reducing Antioxidant Power (FRAP) and 2, 2-di(4-tert-octylphenyl)-1-picrylhydrazyl (DPPH) assays.read more
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References
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Journal ArticleDOI
The ferric reducing ability of plasma (FRAP) as a measure of 'antioxidant power' : the FRAP assay
TL;DR: The FRAP assay offers a putative index of antioxidant, or reducing, potential of biological fluids within the technological reach of every laboratory and researcher interested in oxidative stress and its effects.
Journal ArticleDOI
A simple technique for quantitation of low levels of DNA damage in individual cells
TL;DR: Human lymphocytes were exposed to X-irradiation or treated with H2O2 and the extent of DNA migration was measured using a single-cell microgel electrophoresis technique under alkaline conditions and this technique appears to be sensitive and useful for detecting damage and repair in single cells.
Journal ArticleDOI
Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing.
Raymond R. Tice,E. Agurell,Diana Anderson,B. Burlinson,Andreas Hartmann,H. Kobayashi,Y. Miyamae,Emilio Rojas,J.-C. Ryu,Y. F. Sasaki +9 more
TL;DR: The expert panel reached a consensus that the optimal version of the Comet assay for identifying agents with genotoxic activity was the alkaline (pH > 13) versions of the assay developed by Singh et al.
Journal ArticleDOI
Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.
TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.