Every base matters: assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples
Citations
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1,222 citations
Cites background from "Every base matters: assessing small..."
...(4) 806r Modified GGACTACNVGGGTWTCTAAT Apprill et al....
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...Additionally, further analyses, such as with mock communities (4), could evaluate the extent to which differences in these outliers stem from systematic biases of particular taxa....
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...(4) ITS1f CTTGGTCATTTAGAGGAAGTAA Gardes and Bruns (6) ITS2 GCTGCGTTCTTCATCGATGC White et al....
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...(4) demonstrated improved phylogenetic resolution using the 515f-926r construct; additionally, the percentage of eukaryotic taxa amplified by this construct indicated that in marine or other non-host-associated environments, this construct may be a particularly attractive choice for producing amplicons from all three domains....
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...Specifically, additional degeneracy was added to the 515f primer to reduce bias against Crenarchaeota/Thaumarchaeota (4) and to the 806r primer to minimize the bias against the SAR11 clade (5)....
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382 citations
Cites background from "Every base matters: assessing small..."
...the sampling and library preparation processes to be identified (42, 47, 59, 60)....
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374 citations
Cites background from "Every base matters: assessing small..."
...Many studies have used positive controls comprised of mixtures of cultured organisms (“mock communities”) [23, 96, 111] or known mixtures of free DNA (“mock DNA” samples) [88, 112, 113], both of which make useful controls....
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315 citations
References
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