Exhaustive T-cell repertoire sequencing of human peripheral blood samples reveals signatures of antigen selection and a directly measured repertoire size of at least 1 million clonotypes
Citations
697 citations
567 citations
Cites background from "Exhaustive T-cell repertoire sequen..."
...(12) measured 1 million different TCRB sequences in a peripheral blood sample....
[...]
390 citations
323 citations
Cites background from "Exhaustive T-cell repertoire sequen..."
...To date, most of our knowledge of VDJ recombination and repertoire biases has come from animal models and human peripheral blood analysis, with little comprehensive data on the human thymic TCR repertoire (22, 24, 25)....
[...]
296 citations
References
25,325 citations
"Exhaustive T-cell repertoire sequen..." refers methods in this paper
...The resulting sequence data were aligned against all available exon 2 and 3 nucleotide sequences from the 3.1.0 release of the IMGT/HLA database (Robinson et al. 2003) using ClustalW (Larkin et al. 2007)....
[...]
7,627 citations
"Exhaustive T-cell repertoire sequen..." refers background in this paper
...4 errors per kilobase, but when we added the requirements of (1) double-strand coverage, (2) minimum quality score (Ewing and Green 1998) of Q30, and (3) no...
[...]
...…raw single pass reads, we observed 9.4 errors per kilobase, but when we added the requirements of (1) double-strand coverage, (2) minimum quality score (Ewing and Green 1998) of Q30, and (3) no high-quality discrepancy between strands at any position, the error rate fell to 2.2 errors per kilobase....
[...]
5,334 citations
"Exhaustive T-cell repertoire sequen..." refers background in this paper
...…raw single pass reads, we observed 9.4 errors per kilobase, but when we added the requirements of (1) double-strand coverage, (2) minimum quality score (Ewing and Green 1998) of Q30, and (3) no high-quality discrepancy between strands at any position, the error rate fell to 2.2 errors per kilobase....
[...]
2,858 citations
2,390 citations
Additional excerpts
...Allele assignments (four-digit codes) (Marsh et al. 2010) were based on high-quality exact or synonymous matches at informative nucleotide positions....
[...]