Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
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...After the first description of nucleic acids in exosomes secreted by mast cells (Valadi et al. 2007), numerous groups have analyzed the presence of genetic material in EVs....
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...Exosomes/Extracellular Vesicles and RNA A breakthrough in the field was made in 2007 when it was discovered that exosomes carry nucleic acids, namely mRNA and miRNA (Valadi et al. 2007)....
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...As expected from the inside-in membrane orientation of all secreted EVs (i.e., cytosol inside and extracellular domains facing outside) (Figure 2), in most studies, mRNA are contained inside EVs, as shown by their resistance to RNAse digestion (Valadi et al. 2007)....
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...…with Ca2+ ionophores has also been used in the literature to increase secretion of exosomes by the erythroleukemia cell line K562 (Savina et al. 2003), oligodendroglial cells (Krämer-Albers et al. 2007), DCs (Montecalvo et al. 2012), and mast cells (Raposo et al. 1997, Valadi et al. 2007)....
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...From the time of their initial description in EVs (Ratajczak et al. 2006, Skog et al. 2008, Valadi et al. 2007), we have known that mRNA were not randomly secreted in exosomes, because different sequences were either preferentially secreted or, conversely, retained inside the cells....
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Cites background from "Exosome-mediated transfer of mRNAs ..."
...Extracellular miRNAs have been proposed as a means of intercellular communication (6, 14, 16), and exosome-mediated transfer of miRNA between cells has been reported in cell culture (15) and from plasma-derived microvesicles to endothelial cells in the experimental setting (17)....
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2,762 citations
Cites background from "Exosome-mediated transfer of mRNAs ..."
...Whether the latter phenomenon represents a specific way of elimination of miRISC components from the cell or is indicative of the miRNA-mediated intercellular communication (182) remains to be established....
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2,507 citations
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"Exosome-mediated transfer of mRNAs ..." refers background in this paper
...Hypothetically, the 121 miRNAs that we have found in the mast cell exosomes may interfere with 24,000 mRNAs as it has been suggested that each species may interact with up to 200 mRNA...
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"Exosome-mediated transfer of mRNAs ..." refers background in this paper
...Many cells have the capacity to release exosomes, including reticulocyte...
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1,464 citations