Exploring the trans-cleavage activity of CRISPR-Cas12a for the development of a Mxene based electrochemiluminescence biosensor for the detection of Siglec-5
TL;DR: In this article, a 2D ultra-thin Ti3C2Tx (MXene) based electrochemiluminescence (ECL) biosensor was used for the detection of Siglec-5.
About: This article is published in Biosensors and Bioelectronics.The article was published on 2021-04-15. It has received 46 citations till now. The article focuses on the topics: Loop-mediated isothermal amplification & Trans-activating crRNA.
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TL;DR: In this article , the authors discuss important MXene synthesis routes and critical parameters such as surface functionalization that can dictate the mechanical, electronic, magnetic, and optical properties of MXenes.
66 citations
TL;DR: In this article, the authors highlight recent advances and current challenges to project 2D MXenes for next generation biosensing based on unfurled potentials and novel bio-analytical technologies.
46 citations
TL;DR: In this article , the authors explore some of the most recent advances in the field of CRISPR-powered nanotechnological biosensors, which offer tremendous potential for next-generation diagnostics of multiple targets, especially at the point of care and without any target amplification.
44 citations
TL;DR: In this article, the authors explore some of the most recent advances in the field of CRISPR-powered nanotechnological biosensors, which offer tremendous potential for next-generation diagnostics of multiple targets, especially at the point of care and without any target amplification.
44 citations
TL;DR: In this article, a nanocomposite-based electrochemiluminescence (ECL) biosensor was designed, which realized sensitive detection of the RNAdependent RNA polymerase (RdRp) gene of SARS-CoV-2.
Abstract: The detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is crucial for preventing and controlling infectious diseases and disease treatment. In this work, a Au@Ti3C2@PEI-Ru(dcbpy)32+ nanocomposite-based electrochemiluminescence (ECL) biosensor was rationally designed, which realized sensitive detection of the RNA-dependent RNA polymerase (RdRp) gene of SARS-CoV-2. In addition, a DNA walker was also used to excise the hairpin DNAs under the action of Nb.BbvCI endonuclease. Furthermore, model DNA-Ag nanoclusters (model DNA-AgNCs) were used to quench the initial ECL signal. As a result, the ECL biosensor was used to sensitively detect the SARS-CoV-2 RdRp gene with a detection range of 1 fM to 100 pM and a limit of detection of 0.21 fM. It was indicated that the ECL biosensor had a great application potential for clinical medical detection. Furthermore, the DNA walker amplification also played a reliable candidate strategy for other detection methods.
43 citations
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TL;DR: The recent advances in understanding of the roles of Siglecs in the regulation of immune cell function in infectious diseases, inflammation, neurodegeneration, autoimmune diseases and cancer are described.
Abstract: All mammalian cells display a diverse array of glycan structures that differ from those that are found on microbial pathogens Siglecs are a family of sialic acid-binding immunoglobulin-like receptors that participate in the discrimination between self and non-self, and that regulate the function of cells in the innate and adaptive immune systems through the recognition of their glycan ligands In this Review, we describe the recent advances in our understanding of the roles of Siglecs in the regulation of immune cell function in infectious diseases, inflammation, neurodegeneration, autoimmune diseases and cancer
730 citations
TL;DR: Sialylation leads to increased anti-inflammatory activity of IgG antibodies, facilitates the escape of microorganisms from the host's immune system, and in polymeric form is involved in the regulation of embryogenesis and neuronal growth and function.
598 citations
TL;DR: A sensitive electrogenerated chemiluminescence (ECL) biosensor was developed for detection of exosomes using aptamer modified two-dimensional material Ti3C2 MXenes nanosheets as the ECL nanoprobe because of its large surface area, the excellent conductivity and catalytic properties.
221 citations
TL;DR: This all-in-one detection mode not only bridges the technological gap between biological signal conduction, wireless transmission, and smartphone interface but also improves the portability and the sensitivity (more than one order of magnitude lower than that of traditional CRISPR-Cas12a biosensors).
216 citations
TL;DR: The digital RPA SlipChip provides a simple method to quantify nucleic acids without requiring thermal cycling or kinetic measurements, with potential applications in diagnostics and environmental monitoring under resource-limited settings.
Abstract: In this paper, digital quantitative detection of nucleic acids was achieved at the single-molecule level by chemical initiation of over one thousand sequence-specific, nanoliter isothermal amplification reactions in parallel. Digital polymerase chain reaction (digital PCR), a method used for quantification of nucleic acids, counts the presence or absence of amplification of individual molecules. However, it still requires temperature cycling, which is undesirable under resource-limited conditions. This makes isothermal methods for nucleic acid amplification, such as recombinase polymerase amplification (RPA), more attractive. A microfluidic digital RPA SlipChip is described here for simultaneous initiation of over one thousand nL-scale RPA reactions by adding a chemical initiator to each reaction compartment with a simple slipping step after instrument-free pipet loading. Two designs of the SlipChip, two-step slipping and one-step slipping, were validated using digital RPA. By using the digital RPA SlipCh...
214 citations