Expression and processing of the Hepatitis E virus ORF1 nonstructural polyprotein
Citations
329 citations
Cites background from "Expression and processing of the He..."
...It has been shown that when ORF1 is expressed in a baculovirus expression system, synthesis of several small proteins that correlate with the predictive motifs was observed [124]....
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250 citations
229 citations
Cites background from "Expression and processing of the He..."
...When expressed in insect cells, ORF1 was processed and this was partially blocked by a cell permeable cysteine protease inhibitor (Sehgal et al 2006), but the viral or cellular nature of the protease remained unclear....
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204 citations
References
954 citations
"Expression and processing of the He..." refers background in this paper
...This system also offers post-translational modifications that are similar to those in mammalian cells, yet is capable of expressing much higher quantities of the recombinant protein [18]....
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...Many mammalian proteins have been expressed in their native and active forms using recombinant baculoviruses [18]....
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479 citations
"Expression and processing of the He..." refers background in this paper
...Based on sequence homology, proposed domains and replication mechanism, HEV is closely related to alpha viruses with the Rubella virus being its closest homologue [12]....
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...The numbers on top represent amino acids of the predicted domains numbered according to the ORF1 polyprotein sequence [12]....
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338 citations
"Expression and processing of the He..." refers background in this paper
...Since the ORF1 polyprotein has a predicted cysteine protease domain and cis-acting proteases are found within the nonstructural polyproteins of all other positive-strand RNA viruses [23-28], it is likely that the cysteine protease within the ORF1 polyprotein is responsible for its processing....
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...As is the case with other positive-strand RNA viruses [25-28], these MALDI-TOF analysis of the 35 kDa N-terminal fragment Figure 5 MALDI-TOF analysis of the 35 kDa N-terminal fragment....
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240 citations
"Expression and processing of the He..." refers background in this paper
...Of these, ORF2 encodes an 88-kDa glycoprotein that is the major viral capsid protein [8,9]; ORF3 encodes a phosphoprotein [10], which is involved in cell signaling through MAP kinase pathway [11]....
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177 citations
"Expression and processing of the He..." refers background in this paper
...Of these, ORF2 encodes an 88-kDa glycoprotein that is the major viral capsid protein [8,9]; ORF3 encodes a phosphoprotein [10], which is involved in cell signaling through MAP kinase pathway [11]....
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