Expression of Extracellular Matrix Proteins Accompanies Lesion Growth in a Model of Intimal Reinjury
Hiroyuki Koyama,Michael A. Reidy +1 more
Reads0
Chats0
TLDR
It is demonstrated that the accumulation of extracellular matrix is important in the increase in lesion size after reinjury and that a balance of matrix synthesis and degradation may explain why no change in matrix volume was detected until 28 days after the reinjury.Abstract:
—Reinjury of rat arterial lesions induces an increase in lesion size that is not associated with an increase in cell number. In this study, matrix volume was examined after reinjury to preexisting lesions, and the kinetics of matrix gene expression and activity of proteolytic enzymes in the lesion were evaluated. Volume densitometry in intima showed a significant increase in matrix volume 28 days after the reinjury, although no change was observed at 14 days. Three common vascular matrix molecules, α1(I)procollagen, tropoelastin, and fibronectin, were expressed highly at 7 days after the reinjury. Expression of tropoelastin remained upregulated for the entire 28 days after the reinjury, whereas α1(I)procollagen and fibronectin returned to the control level by 28 days. Protease activity was also increased after reinjury. Within days, a marked increase in urokinase plasminogen activator activity was observed in intima, and this activity decreased to control level by 14 days. The activity of tissue p...read more
Citations
More filters
Journal ArticleDOI
Collagen synthesis in atherosclerosis: too much and not enough.
TL;DR: This review discusses cellular sources of collagen synthesis in atherosclerosis, local and systemic factors modulating collagen gene expression, as well as temporal and spatial patterns of collagen production in human and experimental atherosclerotic lesions.
Journal ArticleDOI
Inhibition of neointimal smooth muscle accumulation after angioplasty by an antibody to PDGF
Journal ArticleDOI
Collagens and atherosclerosis.
TL;DR: Effects as summarized in this short review, are not always, at first sight, consistent and should be kept in mind, though, when considering the response of a cell to collagen.
Journal ArticleDOI
Inhibition of miR-92a improves re-endothelialization and prevents neointima formation following vascular injury.
Jan Marcus Daniel,Daniela Penzkofer,Rebecca Teske,Jochen Dutzmann,Alexander Koch,Wiebke Bielenberg,Angelika Bonauer,Reinier A. Boon,Ariane Fischer,Johann Bauersachs,Eva van Rooij,Stefanie Dimmeler,Daniel Sedding +12 more
TL;DR: It is indicated that inhibition of endothelial miR-92a attenuates neointimal lesion formation by accelerating re-endothelialization and thus represents a putative novel mechanism to enhance the functional recovery following vascular injury.
Journal ArticleDOI
Vein wall remodeling after deep vein thrombosis involves matrix metalloproteinases and late fibrosis in a mouse model
Kristopher B. Deatrick,Jonathan L. Eliason,Erin Lynch,Andrea J. Moore,Nicholas A. Dewyer,Manu R. Varma,Charles G. Pearce,Gilbert R. Upchurch,Thomas W. Wakefield,Peter K. Henke +9 more
TL;DR: In this article, the expression of proteases and collagen involved in early vein wall remodeling was investigated in early venous thrombosis in the mouse, and the results showed that wound healing after DVT is similar to wound healing and is associated with increased procollagen gene expression and total collagen.
References
More filters
Journal ArticleDOI
Extracellular matrix remodeling after balloon angioplasty injury in a rabbit model of restenosis.
Bradley H. Strauss,Robert J. Chisholm,Fred W. Keeley,A I Gotlieb,Richard A. Logan,P W Armstrong +5 more
TL;DR: Significant increases in collagen, elastin, and proteoglycan synthesis, up to 4 to 10 times above control nondamaged contralateral iliac arteries, were noted at 1, 2, and 4 weeks, accompanied by significant increases in synthesis that coincided with the temporal increase in cross-sectional area.
Journal ArticleDOI
Matrix metalloproteinases of vascular wall cells are increased in balloon-injured rat carotid artery * ** *
Nobuya Zempo,Richard D. Kenagy,Y.P. Tina Au,Michelle P. Bendeck,Monika M. Clowes,Michael A. Reidy,Alexander W. Clowes +6 more
TL;DR: The results suggest that the 72 kd and 92 kd gelatinases may be involved in basement membrane and matrix degradation in the media in relation to SMC proliferation and migration, whereas the low-molecular-weight metalloproteinase may have a role in elastin turnover in the adventitia.
Journal ArticleDOI
cDNA cloning by PCR of rat transforming growth factor β-1
Journal ArticleDOI
Purification and characterization of a murine basement membrane collagen-degrading enzyme secreted by metastatic tumor cells.
TL;DR: A type IV collagen-degrading enzyme activity secreted by a highly metastatic mouse tumor was purified by concanavalin A- and typeIV collagen-agarose affinity chromatographies followed by gel filtration on Bio-Gel A-0.5 m, indicating that the enzyme is a hydrophobic protein.
Journal ArticleDOI
Role of endogenous platelet-derived growth factor in arterial smooth muscle cell migration after balloon catheter injury.
TL;DR: The data suggest that platelet-derived growth factor, released by platelets at sites of arterial injury, is an endogenous mediator of smooth muscle cell migration; that plasmin generation, catalyzed by tissue-type plAsminogen activator, is necessary for migration.