Expression, purification, immunogenicity and protective efficacy of a recombinant nucleoside hydrolase from Leishmania donovani, a vaccine candidate for preventing cutaneous leishmaniasis
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Cites methods from "Expression, purification, immunogen..."
...Strong IgG1, IgG2a, and/or IgG2b anti-NH36 antibody responses were described after mice vaccination with the gene of NH36 (23, 24, 27), with the NH36 recombinant protein in combination with saponin (17, 28, 29), CPG and polylactil glycolide particles (39), and the GLA-SE adjuvant (40), or with the NH36 cloned with a sterol 24-C-methyl transferase (NS), and used with GLA-SE adjuvant (30)....
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Additional excerpts
...The results disclosed by our investigations about the potential use of the NH36 antigen in diagnosis and vaccination against leishmaniasis (5, 9–12, 53, 56, 77, 98–101) has inspired the work of other groups, which have used the NH36 recombinant protein for the development of a formulation against human visceral leishmaniasis (15, 94, 95)....
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References
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"Expression, purification, immunogen..." refers background in this paper
...Recently, kala-azar or visceral leishmaniasis was determined to be the neglected tropical 51 disease responsible for the most annual deaths (1), as well as the highest burden of disability-adjusted 52 life years (2)....
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615 citations
"Expression, purification, immunogen..." refers methods in this paper
...160 NanoAcquity UPLC system connected in-line with a Waters Synapt mass spectrometer operating in the 161 data-independent mode (MS E ) (11-13)....
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509 citations
"Expression, purification, immunogen..." refers methods in this paper
...0) software from Waters 180 Corp, using the absolute quantification Identity E algorithm included in the software [(13, 14) ]....
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