Fab-mediated binding of drug-dependent antibodies to platelets in quinidine- and quinine-induced thrombocytopenia.
Reads0
Chats0
TLDR
Findings suggest that binding of drug-induced antibodies to platelets occurs at the Fab domains of the IgG molecule.Abstract:
Platelets coated with quinine- or quinidine-induced antibodies form rosettes around protein A-Sepharose beads and normal platelets form rosettes about protein A-Sepharose beads coated with these antibodies. These reactions occurred only in the presence of sensitizing drug. Platelets also formed rosettes about protein A-Sepharose beads coated with an anti-PIA1 antibody, but drug was not required. Formation of rosettes between antibody-coated platelets and protein A-Sepharose was inhibited by F(ab')2 fragments of goat antibody specific for the Fc portion of human IgG, while rosette formation between antibody-coated protein A-Sepharose and platelets was inhibited by F(ab')2 fragments directed against the F(ab')2 portion of the IgG molecule. Since binding of IgG to protein A is known to occur via the Fc region, these findings suggest that binding of drug-induced antibodies to platelets occurs at the Fab domains of the IgG molecule.read more
Citations
More filters
Journal ArticleDOI
Drug-Induced Immune Thrombocytopenia
TL;DR: The current understanding of pathogenesis is summarized and a guide for diagnosis and management of thrombocytopenia is provided.
Journal ArticleDOI
Drug-induced immune thrombocytopenia: pathogenesis, diagnosis, and management
TL;DR: The most important aspects of patient management are a high index of suspicion and a careful history of drug exposure in an individual who presents with acute, often severe thrombocytopenia of unknown etiology.
Journal ArticleDOI
Drug disposition and drug hypersensitivity.
Journal ArticleDOI
Immune hemolytic anemia associated with drug therapy.
TL;DR: The most common drugs to cause DIIHA are anti-microbials, which are associated with drug-dependent antibodies, and the most common drug to cause AIHA is fludarabine.
Journal ArticleDOI
Prospective evaluation of the clinical usefulness of an antigen-specific assay (MAIPA) in idiopathic thrombocytopenic purpura and other immune thrombocytopenias.
TL;DR: The experience suggests that MAIPA assays are useful in the laboratory assessment of thrombocytopenia, should be performed before therapy, and that some patients with 'nonimmune' thromBocy topenia may have genuine antiplatelet antibodies.
References
More filters
Journal ArticleDOI
Quinidine-induced thrombocytopenia and leukopenia: demonstration and characterization of distinct antiplatelet and antileukocyte antibodies
TL;DR: A patient with the rare syndrome of simultaneous quinidine-induced thrombocytopenia and leukopenia was studied, and a drug-dependent antileukocyte antibody was demonstrated by leukoagglutination and by granulocyte immunofluorescence.
Journal ArticleDOI
Coombs'-test positivity induced by drugs. Mechanisms of immunologic reactions and red cell destruction.
Joseph D. Croft,Scott N. Swisher,Bruce C. Gilliland,Richard F. Bakemeier,John P. Leddy,Robert I. Weed +5 more
Journal ArticleDOI
Direct quantitation of platelet-associated IgG by electroimmunoassay
Thomas J. Kunicki,Thomas J. Kunicki,Mary B. Koenig,Mary B. Koenig,Susan M. Kristopeit,Susan M. Kristopeit,Richard H. Aster,Richard H. Aster +7 more
TL;DR: Electroimmunoassay for PAIgG can be performed on patients with platelet counts as low as 2000/microliters, yields results in less than 24 hr, is highly reproducible, and appears to provide a useful tool for the evaluation of patients with immunologically mediated thrombocytopenia.
Journal ArticleDOI
Drug-dependent and non-drug-dependent antiplatelet antibody in drug-induced immunologic thrombocytopenic purpura.
TL;DR: The mechanism of drug-dependent immunologic thrombocytopenic purpura (DITP) was investigated by studying the sera of four patients with classic DITP using a solid-phase radioimmunoassay with 125I-staphylococcal protein A.
Journal ArticleDOI
Structural features of the quinidine and quinine molecules necessary for binding of drug-induced antibodies to human platelets
Douglas J. Christie,Douglas J. Christie,Douglas J. Christie,Robert W. Weber,Robert W. Weber,Robert W. Weber,Patrick C. Mullen,Patrick C. Mullen,Patrick C. Mullen,James M. Cook,James M. Cook,James M. Cook,Richard H. Aster,Richard H. Aster,Richard H. Aster +14 more
TL;DR: Results provide further evidence for heterogeneity among drug-induced platelet antibodies by demonstrating that noncross-reactive antibodies are dependent for their activity on a specific configuration at the optically active C(9)-hydroxyl position, and that some of these also require the methoxy group for full reactivity.