Journal ArticleDOI
Factors influencing cord blood viability assessment before cryopreservation.
Michael Solomon,Jonathan D. Wofford,Jonathan D. Wofford,Cory Johnson,Cory Johnson,Donna Regan,Donna Regan,Michael H. Creer +7 more
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TLDR
Global viability assessment may not reflect viability of white blood cell (WBC) subsets, CD34+ cell viability, or hematopoietic stem/progenitor cells function.About:
This article is published in Transfusion.The article was published on 2010-04-01. It has received 35 citations till now. The article focuses on the topics: Viability assay & Propidium iodide.read more
Citations
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Morphological observation and analysis using automated image cytometry for the comparison of trypan blue and fluorescence-based viability detection method
TL;DR: Comparison of TB exclusion and fluorescence-based viability detection methods using image cytometry to observe morphological changes due to the effect of TB on dead cells showed that as the viability of a naturally-dying Jurkat cell sample decreased below 70 %, many TB-stained cells began to exhibit non-uniform morphological characteristics.
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Evaluation of the Cell Viability of Human Wharton's Jelly Stem Cells for Use in Cell Therapy
Ingrid Garzón,Bárbara Pérez-Köhler,Juan Garrido-Gómez,Víctor Carriel,Renato Nieto-Aguilar,Miguel Angel Martin-Piedra,Natalio García-Honduvilla,Julia Buján,António Campos,Miguel Alaminos +9 more
TL;DR: Human umbilical cord Wharton's jelly stem cells (HWJSCs) are gaining attention as a possible clinical source of mesenchymal stem cells for cell therapy and tissue engineering due to their high accessibility, expansion potential, and plasticity.
Journal ArticleDOI
Current thawing and infusion practice of cryopreserved cord blood: the impact on graft quality, recipient safety, and transplantation outcomes
TL;DR: Methods of handling, thawing, and infusion of cord blood (CB) products vary substantially among thaw/transplant centers (TCs) especially when the TC is unable to follow recommendations due to foreseeable technical, quality, and/or clinical factors.
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The Assessment of Parameters Affecting the Quality of Cord Blood by the Appliance of the Annexin V Staining Method and Correlation with CFU Assays.
Teja Falk Radke,David Monteiro Barbosa,Richard Duggleby,Riccardo Saccardi,Sergio Querol,Gesine Kögler +5 more
TL;DR: Both Annexin V staining method and CFU-assays with defined seeding density are reliable means leading to a better prediction of the final potency of cord blood potency.
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Direct concentration and viability measurement of yeast in corn mash using a novel imaging cytometry method
TL;DR: The proposed method enables specific fluorescence detection of viable and nonviable yeasts, which can generate precise results for concentration and viability of yeast in corn mash, and can provide an essential tool for research and development in the biofuel industry.
References
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Journal ArticleDOI
The ISHAGE guidelines for CD34+ cell determination by flow cytometry. International Society of Hematotherapy and Graft Engineering.
TL;DR: The four-parameter flow methodology adopted by ISHAGE for validation in a multicenter study in North America is described and its utility on a variety of flow cytometers in clinical laboratories and its reproducibility between transplant centers are established.
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Transplantation of unrelated donor umbilical cord blood in 102 patients with malignant and nonmalignant diseases: influence of CD34 cell dose and HLA disparity on treatment-related mortality and survival.
John E. Wagner,Juliet N. Barker,Todd E. DeFor,K. Scott Baker,Bruce R. Blazar,Cindy R. Eide,Anne I. Goldman,John H. Kersey,William Krivit,Margaret L. MacMillan,Paul J. Orchard,Charles Peters,Daniel J. Weisdorf,Norma K.C. Ramsay,Stella M. Davies +14 more
TL;DR: There is a high probability of survival in recipients of UCB grafts that are disparate in no more than 2 human leukocyte antigens when the grafts contain at least 1.7 x 10(5) CD34(+) cells per kilogram of recipient's body weight, and graft selection should be based principally on CD34 cell dose when multiple UCB units exist with an HLA disparity of 2 or less.
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Processing and cryopreservation of placental/umbilical cord blood for unrelated bone marrow reconstitution.
Pablo Rubinstein,Ludy Dobrila,Richard E. Rosenfield,John W. Adamson,Giovanni Migliaccio,Anna Rita Migliaccio,Patricia E. Taylor,Cladd E. Stevens +7 more
TL;DR: Almost all the hematopoietic colony-forming cells present in PCB units can be recovered in a uniform volume of 20 ml by using rouleaux formation induced by hydroxyethyl starch and centrifugation to reduce the bulk of erythrocytes and plasma and, thus, concentrate leukocytes.
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Single platform flow cytometric absolute CD34+ cell counts based on the ISHAGE guidelines
TL;DR: The basic ISHAGE method is modified with the addition of a known number of Flow-Count fluorospheres to produce an absolute count of viable CD34+ cells and may improve the interlaboratory reproducibility of CD34 determinations due to the reduction in sample handling and calculation of results.