Journal ArticleDOI
Fast and sensitive silver staining of DNA in polyacrylamide gels
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TLDR
The photochemically derived silver stain of nucleic acids in polyacrylamide gels originally described by Merril et al. was modified to reduce unspecific background staining and increase sensitivity and this improved staining procedure is applied for the routine analysis of complex DNA profiles generated by DNA amplification fingerprinting (DAF).About:
This article is published in Analytical Biochemistry.The article was published on 1991-07-01. It has received 2879 citations till now. The article focuses on the topics: Silver stain & Silver nitrate.read more
Citations
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Identification of a Gene That Causes Primary Open Angle Glaucoma
Edwin M. Stone,John H. Fingert,Wallace L.M. Alward,Thai Nguyen,Jon R. Polansky,Sara L.F. Sunden,Darryl Y. Nishimura,Abbot F. Clark,Arne M. Nystuen,Brian E. Nichols,David A. Mackey,Robert Ritch,Jeffrey W. Kalenak,E. Randy Craven,Val C. Sheffield +14 more
TL;DR: A gene encoding a trabecular meshwork protein (TIGR) mapped to the narrowest disease interval by STS content and radiation hybrid mapping and will aid in early diagnosis of glaucoma.
Journal ArticleDOI
A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars
A. Prevost,Mike J. Wilkinson +1 more
TL;DR: It is concluded that ISSR-PCR provides a quick, reliable and highly informative system for DNA fingerprinting that is amenable for routine applications.
Journal ArticleDOI
Pendred syndrome is caused by mutations in a putative sulphate transporter gene (PDS)
Lorraine A. Everett,Benjamin Glaser,John S. Beck,Jacquelyn R. Idol,Andreas Buchs,Ma’ayan Heyman,Faiad Adawi,Elizur Hazani,Elias Nassir,Andreas D. Baxevanis,Val C. Sheffield,Eric D. Green +11 more
TL;DR: These studies provide compelling evidence that defects in pendrin cause Pendred syndrome thereby launching a new area of investigation into thyroid physiology, the pathogenesis of congenital deafness and the role of altered sulphate transport in human disease.
Journal ArticleDOI
DNA amplification fingerprinting using very short arbitrary oligonucleotide primers.
TL;DR: This approach, DNA amplification fingerprinting (DAF), does not depend on cloning or DNA sequence information and can generate fingerprints from DNA of viral, bacterial, fungal, plant and animal origins.
Journal ArticleDOI
Detection of single sequence repeat polymorphisms in denaturing polyacrylamide sequencing gels by silver staining
TL;DR: An improved low-cost method for silver staining is presented and it is compared to 2 other methods for their ability to detect simple sequence repeat polymorphisms in denaturing polyacrylamide gels bound to glass plates.
References
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Journal ArticleDOI
Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels
TL;DR: A new modification of silver staining is presented which utilizes two chemical properties of thiosulfate: image enhancement by pretreatment of fixed gels, and formation of soluble silver complexes which prevents unspecific background staining during image development.
Journal ArticleDOI
Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins
TL;DR: A new silver stain for electrophoretically separated polypeptides can be rapidly and easily used and can detect as little as 0.01 nanogram of protein per square millimeter when employed with two-dimensional electrophoresis.
Journal ArticleDOI
Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining
TL;DR: Comparison of isoelectric focusing and SDS‐containing gels and a simple spot test on thin gellayers show that the detection sensitivity depends not so much on the type of proteins but rather on their structure.
Journal ArticleDOI
DNA amplification fingerprinting using very short arbitrary oligonucleotide primers.
TL;DR: This approach, DNA amplification fingerprinting (DAF), does not depend on cloning or DNA sequence information and can generate fingerprints from DNA of viral, bacterial, fungal, plant and animal origins.
Journal Article
Polymerase chain reaction amplification products separated on rehydratable polyacrylamide gels and stained with silver
TL;DR: Sensitivity, using a silver stain, indicated that one could readily distinguish less than 10 pg of DNA per mm width on the gels.