Journal ArticleDOI
Fermentation optimization for the production of lovastatin by Aspergillus terreus: use of response surface methodology
J.L. Casas López,J.A. Sánchez Pérez,J. M. Fernández Sevilla,F.G. Acién Fernández,E. Molina Grima,Yusuf Chisti +5 more
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TLDR
A Box-Behnken experimental design was used to investigate the effects of five factors (oxygen content in the gas phase, concentrations of C, N and P, and fermentation time) on the concentrations of biomass and lovastatin produced in batch cultures of Aspergillus terreus as discussed by the authors.Abstract:
A Box-Behnken experimental design was used to investigate the effects of five factors—ie oxygen content in the gas phase; concentrations of C, N and P; and fermentation time—on the concentrations of biomass and lovastatin produced in batch cultures of Aspergillus terreus. The values of the various factors in the experiment ranged widely, as follows: 20-80% (v/v) oxygen in the aeration gas; 8-48 g dm −3 C-concentration; 0.2-0.6 g dm −3 N-concentration; 0.5-2.5 g dm −3 phosphate-concentration; and 7-11 days fermentation time. No previous work has used statistical analysis in documenting the interactions between oxygen supply and nutrient concentrations in lovastatin production. The Box-Behnken design identified the oxygen content in the gas phase as the principal factor influencing the production of lovastatin. Both a limitation and excess of oxygen reduced lovastatin titers. A medium containing 48 g dm −3 C supplied as lactose, 0.46 g dm −3 N supplied as soybean meal, and 0.79 g dm −3 phosphate supplied as KH2PO4, was shown to support high titers (∼230 mg dm −3 )o f lovastatin in a7 -day fermentation in oxygen-rich conditions (80% v/v oxygen in the aeration gas). Under these conditions, the culture medium had excess carbon but limiting amounts of nitrogen. The optimized fermentation conditions raised the lovastatin titer by four-fold compared with the worst-case scenario within the range of factors investigated. 2004 Society of Chemical Industryread more
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Biotechnology-a sustainable alternative for chemical industry.
Maria Gavrilescu,Yusuf Chisti +1 more
TL;DR: Environmental and economic benefits that biotechnology can offer in manufacturing, monitoring and waste management are highlighted and the following benefits include: greatly reduced dependence on nonrenewable fuels and other resources; reduced potential for pollution of industrial processes and products.
Journal ArticleDOI
Biotechnological production and applications of statins.
TL;DR: Advances in the biochemistry and genetics of lovastatin have allowed the development of new methods for the production of simvastatin, the second leading statin in the market, which is a Lovastatin semisynthetic derivative.
Journal ArticleDOI
Pellet morphology, culture rheology and lovastatin production in cultures of Aspergillus terreus.
J.L. Casas López,J.A. Sánchez Pérez,J. M. Fernández Sevilla,E.M. Rodríguez Porcel,Yusuf Chisti +4 more
TL;DR: Both an upper limit on agitation intensity and a high level of dissolved oxygen are essential for attaining high titers of lovastatin in fungal pellet morphology and broth rheology.
Journal ArticleDOI
Advances in photobioreactors for intensive microalgal production: configurations, operating strategies and applications
TL;DR: This review aims at analyzing and classifying the most recent advances and the several novel approaches to the design, development, control and modeling of photobioreactors.
Journal ArticleDOI
A new approach of pellet formation of a filamentous fungus -Rhizopus oryzae.
TL;DR: A new approach to form pellets has been developed using only potato dextrose broth, soybean peptone, and calcium carbonate allowing for pellet size to be controlled by adjusting inoculum size and the concentrations of potato deXTrose broth.
References
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Journal ArticleDOI
Monitoring of selected metabolites and biotransformation products from fermentation broths by high-performance liquid chromatography
TL;DR: In this paper, a reversed-phase high-performance liquid chromatographic method with UV-photodiode array or multiwavelength detection was developed for the analysis of secondary fungal metabolites mevinolin (also known as monacolin K, lovastatin or Mevacor) and fumagillin in fermentation broths.
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Interactions of cell morphology and transport processes in the lovastatin fermentation
TL;DR: Experiments indicate considerable potential advantages to the pellet morphology from the standpoint of oxygen transport processes in Aspergillus terreus fermentation.
Journal ArticleDOI
Biosynthesis of ML-236C and the hypocholesterolemic agents compactin by Penicillium aurantiogriseum and lovastatin by Aspergillus terreus: determination of the origin of carbon, hydrogen and oxygen atoms by 13C NMR spectrometry and observation of unusual labelling of acetate-derived oxygens by 18O2
TL;DR: In this article, the regiochemical distribution of 2H, 13C and 18O is determined by 13C NMR spectrometry, and a mechanism is proposed to account for the formation of the bicyclic ring system in these compounds.
Journal ArticleDOI
Influence of Increased Dissolved Oxygen Tensions by Agitation on Secondary Metabolite Production by a Mutant of Aspergillus Terreus in a 5L Fermentor
TL;DR: While increasing DO levels above 20% of saturation in 24-48hrs, this fermentor operation was found to enhance a 1-day earlier production of lovastatin, in comparison with shaking-flask data, mainly attributed to the formation of compact pellets rather than viscous and filamentous mycelium growth.
Patent
Enzymatic deacylation of simvastatin
TL;DR: A process for the enzymatic deacylation of compound (II> to form compounds of formula (Ill) and (IV) is described in this article, where a process is described for the enzyme-based decoylation.