scispace - formally typeset
Search or ask a question
Journal ArticleDOI

Fermentative production and kinetics of cellulase protein on Trichoderma reesei using sugarcane bagasse and rice straw

01 Jan 2006-African Journal of Biotechnology (Academic Journals (Kenya))-Vol. 5, Iss: 20
TL;DR: This is the first attempt of combining the synthetic substrate (xylose, lactose) with natural substrate (sugarcane bagasse, rice straw) and the mixture of substrates produced the highest maximal enzyme activity on cellulose with xylose.
Abstract: Cellulase a multienzyme made up of several proteins finds extensive applications in food, fermentation and textile industries. Trichoderma reesei is an efficient producer of cellulase protein. The comparative study was made on various carbon sources on the production of cellulase using strains of T. reesei QM 9414, 97.177 and Tm3. Pretreatment of sugarcane bagasse and rice straw offers very digestible cellulose and potentially less inhibition. Cellulase production was enhanced by multiple carbon sources because of diauxic pattern of utilization of substrates. This is the first attempt of combining the synthetic substrate (xylose, lactose) with natural substrate (sugarcane bagasse, rice straw). The mixture of substrates produced the highest maximal enzyme activity on cellulose with xylose, cellulose with lactose, bagasse with xylose, bagasse with lactose, rice straw with xylose and rice straw with lactose. In addition Monod growth kinetics and Leudeking piret product formation kinetics were studied using T. reesei with optimized medium under optimized conditions of inoculum concentration, D.O. level, agitator speed, temperature and pH.

Content maybe subject to copyright    Report

Citations
More filters
Journal ArticleDOI
01 Mar 2017
TL;DR: In this paper, the use of sugarcane bagasse as cellulosic substrate, in combination with passion fruit rind flour, as co-substrate and corn steep liquor as nitrogen source can be successfully employed for the production of avicelases by Bacillus sp. SMIA-2.
Abstract: The application of wastes from the food processing industry as carbon sources in enzyme production processes reduces the cost of production, and helps solving problems related to their disposal. In this work, we demonstrated that the use of sugarcane bagasse as cellulosic substrate, in combination with passion fruit rind flour, as co-substrate and corn steep liquor as nitrogen source can be successfully employed for the production of avicelases (avicel-hydrolyzing enzymes) by Bacillus sp. SMIA-2. This would promote the use of these agricultural byproducts as novel and cost-effective culture media for the production of the enzyme. The maximum avicelase activity was obtained when Bacillus sp SMIA-2 was grown in liquid medium containing 0.625% (w/v) sugarcane bagasse, 0.625% (v/v) corn steep liquor and 075% (w/v) passion fruit peel flour for 168 hours.

5 citations


Cites background from "Fermentative production and kinetic..."

  • ...Muthuvelayudham and Viruthagiri [20] analyzed the effect of different carbon sources such as glucose, lactose and xylose on submerged fermentation, using isolates of T....

    [...]

  • ...Muthuvelayudham and Viruthagiri [20] analyzed the effect of different carbon sources such as glucose, lactose and xylose on submerged fermentation, using isolates of T. reesei, and verified that the combination of cellulose and xylose led to the best level of cellulase production....

    [...]

  • ...Muthuvelayudham R, Viruthagiri T (2006) Fermentative production and kinetics of cellulase protein on Trichoderma reesei using sugarcane bagasse and rice straw....

    [...]

Journal ArticleDOI
TL;DR: Agricultural by-products, rice straw, wheat bran juice, and soybean residue, were used as substrates for cellulase production using Rhizopus stolonifer var.
Abstract: Agricultural by-products, rice straw, wheat bran juice, and soybean residue, were used as substrates for cellulase production using Rhizopus stolonifer var. reflexus TP-02. The culture medium was optimized though uniform design experimentation during shaking flask fermentation, and the ideal formulation obtained for filter paper enzyme (FPase) production was 10 % bran diffusion juice, 1 % rice straw, 0.17 % urea, 0.17 % soybean residue, 0.11 % KH2PO4, and 0.027 % Tween 80, and the maximal FPase activity in the culture supernatant was 13.16 U/mL at an incubation time of 3 days. A kinetic model for cellulase production in batch fermentation was subsequently developed. The unstructured kinetic model considered three responses, namely biomass, cellulase, and sugar. Models for the production of three types of cellulase components (i.e., endoglucanases, cellobiohydrolases, and β-glucosidases) were established to adequately describe the cellulase production pattern. It was found that the models fitted the experimental data well under pH 5.0 and 6.0, but only the avicelase production model predicted the experimental data under pH-uncontrolled conditions.

5 citations


Cites background from "Fermentative production and kinetic..."

  • ...Agricultural processing wastes, such as potato pulp (from the starch industry) [36], sugarcane bagasse, and rice straw [37], have been approved to be effective for T....

    [...]

Journal ArticleDOI
TL;DR: The results of this work demonstrate that Ulva rigida can be saccharified and fermented in a single step by a terrestrial fungus in 72 h under submerged fermentation to provide high-quality proteins suitable to produce feeds.
Abstract: The green macroalgae biomass accumulated in eutrophicated coastal waters has negative ecological and economic impacts. Green macroalgae have an interesting nutritional profile that can be exploited for production of feeds. Bioconversion of green seaweed by microbial fermentation allows the production of microbial biomass protein. This research aimed to study the saccharification and fermentation of green macroalgae biomass by a cellulolytic fungus to provide a product with a nutritional added-value. For this purpose, green seaweed Ulva rigida was directly saccharified and fermented in batch using the filamentous fungus Trichoderma reesei Rut-C30. The fermentation product obtained from the growth of T. reesei Rut-C30 on Ulva rigida as the sole carbon source in 72 h was called fungal biomass protein (FBP) from fermented Ulva rigida. FBP from fermented Ulva rigida contained all essential amino acids and compared favorably with the FAO guideline profiles. Besides, the content of limiting amino acid methionine of FBP from Ulva rigida increased 4-folds compared to the raw alga (from 0.65% to 3% of the total protein content) and was similar to the methionine levels of ovalbumin protein. The in vitro digestibility of FBP from fermented Ulva rigida increased from 71% to 94% compared to the raw alga, and was higher than that of leguminous seeds and similar to that of soybean meal. The results of our work demonstrate that Ulva rigida can be saccharified and fermented in a single step by a terrestrial fungus in 72 h under submerged fermentation to provide high-quality proteins suitable to produce feeds.

5 citations

Journal ArticleDOI
TL;DR: In this article, a fungal fermentation-based strategy for the economic enzymatic conversion of pineapple peel into fermentable sugars was established, and the effects of medium pH, cultivation time, and passion fruit peel concentration on β-glucosidase production were evaluated using a central composite rotational design (CCRD) combined with response surface methodology (RSM).
Abstract: Efficient, low-cost enzymatic hydrolysis of lignocellulosic biomass is essential for cost-effective production of bioethanol. The aim of this study was to establish a fungal fermentation-based strategy for the economic enzymatic conversion of pineapple peel into fermentable sugars. Trichoderma viride was grown on passion fruit peel in order to improve its β-glucosidase production, and a crude extract was then used to hydrolyze pineapple peel. The effects of medium pH, cultivation time, and passion fruit peel concentration on β-glucosidase production were evaluated using a central composite rotational design (CCRD) combined with response surface methodology (RSM). Optimal β-glucosidase activity of 2.40 U mL−1 was found after 6.5 days of cultivation in medium at pH 6.0, containing 2.0 % passion fruit peel. Saccharification of pineapple peel was also optimized by RSM and CCRD with respect to pH, temperature, β-glucosidase concentration, and reaction time and proceeded optimally at pH 4.0, 55 °C, with a β-glucosidase loading of 31.25 U g−1 dry feedstock and 75 h of reaction. Under these conditions, T. viride crude extract hydrolyzed pineapple peel with a glucose yield of 65.3 %. This study therefore presents passion fruit peel as an attractive raw material for the production of β-glucosidases. In addition, it describes an improved, effective, and low-cost enzymatic method for the production of fermentable sugars from pineapple peel, an abundant and inexpensive agro-industrial waste.

5 citations


Cites methods from "Fermentative production and kinetic..."

  • ...The pH was then neutralized with dilute phosphoric acid and the mixture was dried at 70 °C [30]....

    [...]

Journal Article
TL;DR: In this paper, the authors investigate the steps that increase cellulase production and assess the effect of these steps on the reduction of cellulase degradation in new microbial sources of cellulas.
Abstract: Studies on new microbial sources of cellulas e and accurate assessment of the steps that increase cellulase production are e ssential strategies to reduce c...

4 citations

References
More filters
Journal ArticleDOI
TL;DR: The conversion of both cellulose and hemicellulose for the production of fuel ethanol is being studied intensively, with a view to developing a technically and economically viable bioprocess.

492 citations


"Fermentative production and kinetic..." refers background in this paper

  • ...…growth rate (day)-1; µ, specific growth rate (day)–1; Ks, Monod constant; P, product concentration (g/l); α and β, Leudeking Piret constants; and X, substrate concentration (g/l). a number of applications including animal feed, pharmaceutical and textile industries (Aristidou and Penttilä, 2000)....

    [...]

  • ...a number of applications including animal feed, pharmaceutical and textile industries (Aristidou and Penttilä, 2000)....

    [...]

Journal ArticleDOI
TL;DR: RNA slot blot experiments showed that no expression could be observed on glucose-containing medium and that high glucose levels abolish the inducing effect of sophorose, showing that distinct and clear-cut mechanisms of induction and glucose repression regulate cellulase expression in an actively growing fungus.
Abstract: Basic features of regulation of expression of the genes encoding the cellulases of the filamentous fungus Trichoderma reesei QM9414, the genes cbh1 and cbh2 encoding cellobiohydrolases and the genes egl1, egl2 and egl5 encoding endoglucanases, were studied at the mRNA level. The cellulase genes were coordinately expressed under all conditions studied, with the steady-state mRNA levels of cbh1 being the highest. Solka floc cellulose and the disaccharide sophorose induced expression to almost the same level. Moderate expression was observed when cellobiose or lactose was used as the carbon source. It was found that glycerol and sorbitol do not promote expression but, unlike glucose, do not inhibit it either, because the addition of 1 to 2 mM sophorose to glycerol or sorbitol cultures provokes high cellulase expression levels. These carbon sources thus provide a useful means to study cellulase regulation without significantly affecting the growth of the fungus. RNA slot blot experiments showed that no expression could be observed on glucose-containing medium and that high glucose levels abolish the inducing effect of sophorose. The results clearly show that distinct and clear-cut mechanisms of induction and glucose repression regulate cellulase expression in an actively growing fungus. However, derepression of cellulase expression occurs without apparent addition of an inducer once glucose has been depleted from the medium. This expression seems not to arise simply from starvation, since the lack of carbon or nitrogen as such is not sufficient to trigger significant expression.

483 citations


"Fermentative production and kinetic..." refers background in this paper

  • ...Hypercelluloytic mutant strains secrete large amounts of cellulases (Ilmen et al., 1997)....

    [...]

Journal ArticleDOI
TL;DR: This mini-review summarises the considerable amount of data accumulated over the past three decades regarding promoters of genes encoding hydrolytic enzymes, inducer molecules, and models for the recognition of the insoluble substrates cellulose and xylan.
Abstract: The genus Trichoderma comprises a group of filamentous ascomycetes that are now widely used in industrial applications because of their ability to produce extracellular hydrolases in large amounts. In addition, strong inducible promoters together with high secretory capacity have made Trichoderma an attractive host for heterologous protein production. Several promoters of genes encoding hydrolytic enzymes have been investigated in detail regarding their cis-acting elements and trans-acting factors. Potent inducer molecules, for both xylanolytic and cellulolytic enzyme systems, have been identified and characterized. Furthermore, models for the recognition of the insoluble substrates cellulose and xylan have been developed based on a large set of experiments. This mini-review summarises the considerable amount of data accumulated over the past three decades.

207 citations


"Fermentative production and kinetic..." refers background in this paper

  • ...The genus Trichoderma, filamentous ascomycetes are widely used in industrial applications because of high secretory capacity and inducible promoting characteristics (Mach and Zeilinger, 2003)....

    [...]

Journal ArticleDOI
TL;DR: The growth and enzyme production by Trichoderma reesei Rut C-30 using different lignocellulosic materials as carbon source were investigated and it was found that endoglucanase and endoxylanse activities were produced throughout the cultivations, whereas α-arabinosidase was induced late during the cultivation.

157 citations


"Fermentative production and kinetic..." refers background in this paper

  • ...Biosynthesis of cellulase was also made on lignocellulo-sic materials such as sugar beet pulp and alkaline extracted sugar beet pulp and cellulose (Olsson et al., 2003)....

    [...]

  • ...The general trend is that more cellulose in the mixture results in higher levels of endoglucanase (Olsson et al., 2003)....

    [...]

Journal ArticleDOI
TL;DR: The filamentous fungus Penicillium brasilianum IBT 20888 was cultivated on a mixture of 30 g l−1 cellulose and 10 g xylan for 111 h and the resulting culture filtrate was used for protein purification and revealed similarity in molecular mass, pI and hydrolytic properties suggested that these two enzymes were identical, but the smaller was lacking the cellulose-binding domain or an essential part of it.

134 citations