Fermentative production and kinetics of cellulase protein on Trichoderma reesei using sugarcane bagasse and rice straw
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TLDR
This is the first attempt of combining the synthetic substrate (xylose, lactose) with natural substrate (sugarcane bagasse, rice straw) and the mixture of substrates produced the highest maximal enzyme activity on cellulose with xylose.Abstract:
Cellulase a multienzyme made up of several proteins finds extensive applications in food, fermentation and textile industries. Trichoderma reesei is an efficient producer of cellulase protein. The comparative study was made on various carbon sources on the production of cellulase using strains of T. reesei QM 9414, 97.177 and Tm3. Pretreatment of sugarcane bagasse and rice straw offers very digestible cellulose and potentially less inhibition. Cellulase production was enhanced by multiple carbon sources because of diauxic pattern of utilization of substrates. This is the first attempt of combining the synthetic substrate (xylose, lactose) with natural substrate (sugarcane bagasse, rice straw). The mixture of substrates produced the highest maximal enzyme activity on cellulose with xylose, cellulose with lactose, bagasse with xylose, bagasse with lactose, rice straw with xylose and rice straw with lactose. In addition Monod growth kinetics and Leudeking piret product formation kinetics were studied using T. reesei with optimized medium under optimized conditions of inoculum concentration, D.O. level, agitator speed, temperature and pH.read more
Citations
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Gamma and UV radiation induced mutagenesis in Trichoderma reesei to enhance cellulases enzyme activity
TL;DR: The developed mutant from gama mutagenesis way is the best mutant for Ccase, CMCase, Avicellase and Fpase activities and could be applied to obtain potent fungal mutants for more enzymes production.
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Atividade celulolítica de fungos isolados de bagaço de cana-de-açúcar e madeira em decomposição
TL;DR: Although T. reesei QM9414 showed the highest total cellulolytic activity, some isolates showed higher endoglucanase activities, and biodiversity found in habitats such as sugarcane bagasse can provide strains of cellulolytics fungi with great biotechnological potential.
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Production of cellulase - a review
TL;DR: This present review paper discusses discussion attempted on “cellulase production” by submerged and solid state fermentation using various types of bacteria and fungi with different types of agro wastes.
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Enhanced Production of β-glucosidase by New Strain Aspergillus protuberus on Solid State Fermentation in Rice Husk
TL;DR: In this paper, the authors investigated the role of the cost of cellulosic substrates in determining the economy of the saccharification process of polysaccharides to glucose by microbial hydrolytic enzymes.
References
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Metabolic engineering applications to renewable resource utilization.
Aristos Aristidou,Merja Penttilä +1 more
TL;DR: The conversion of both cellulose and hemicellulose for the production of fuel ethanol is being studied intensively, with a view to developing a technically and economically viable bioprocess.
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Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei.
TL;DR: RNA slot blot experiments showed that no expression could be observed on glucose-containing medium and that high glucose levels abolish the inducing effect of sophorose, showing that distinct and clear-cut mechanisms of induction and glucose repression regulate cellulase expression in an actively growing fungus.
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Regulation of gene expression in industrial fungi: Trichoderma.
Robert L. Mach,Susanne Zeilinger +1 more
TL;DR: This mini-review summarises the considerable amount of data accumulated over the past three decades regarding promoters of genes encoding hydrolytic enzymes, inducer molecules, and models for the recognition of the insoluble substrates cellulose and xylan.
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Influence of the carbon source on production of cellulases, hemicellulases and pectinases by Trichoderma reesei Rut C-30
TL;DR: The growth and enzyme production by Trichoderma reesei Rut C-30 using different lignocellulosic materials as carbon source were investigated and it was found that endoglucanase and endoxylanse activities were produced throughout the cultivations, whereas α-arabinosidase was induced late during the cultivation.
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Purification and characterization of five cellulases and one xylanase from Penicillium brasilianum IBT 20888
TL;DR: The filamentous fungus Penicillium brasilianum IBT 20888 was cultivated on a mixture of 30 g l−1 cellulose and 10 g xylan for 111 h and the resulting culture filtrate was used for protein purification and revealed similarity in molecular mass, pI and hydrolytic properties suggested that these two enzymes were identical, but the smaller was lacking the cellulose-binding domain or an essential part of it.