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Journal ArticleDOI

Fermentative production and kinetics of cellulase protein on Trichoderma reesei using sugarcane bagasse and rice straw

01 Jan 2006-African Journal of Biotechnology (Academic Journals (Kenya))-Vol. 5, Iss: 20
TL;DR: This is the first attempt of combining the synthetic substrate (xylose, lactose) with natural substrate (sugarcane bagasse, rice straw) and the mixture of substrates produced the highest maximal enzyme activity on cellulose with xylose.
Abstract: Cellulase a multienzyme made up of several proteins finds extensive applications in food, fermentation and textile industries. Trichoderma reesei is an efficient producer of cellulase protein. The comparative study was made on various carbon sources on the production of cellulase using strains of T. reesei QM 9414, 97.177 and Tm3. Pretreatment of sugarcane bagasse and rice straw offers very digestible cellulose and potentially less inhibition. Cellulase production was enhanced by multiple carbon sources because of diauxic pattern of utilization of substrates. This is the first attempt of combining the synthetic substrate (xylose, lactose) with natural substrate (sugarcane bagasse, rice straw). The mixture of substrates produced the highest maximal enzyme activity on cellulose with xylose, cellulose with lactose, bagasse with xylose, bagasse with lactose, rice straw with xylose and rice straw with lactose. In addition Monod growth kinetics and Leudeking piret product formation kinetics were studied using T. reesei with optimized medium under optimized conditions of inoculum concentration, D.O. level, agitator speed, temperature and pH.

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Citations
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Journal ArticleDOI
TL;DR: In this article, the authors highlight the recent advances in the treatment and value addition of lignocellulosic wastes (LCW) with main focus on domestic and agro-industrial residues.
Abstract: This study highlights the recent advances in the treatment and value addition of lignocellulosic wastes (LCW) with main focus on domestic and agro-industrial residues. Mechanical, physical and biological treatment systems are brought into perspective. The main value-added products from lignocellulosic wastes are summarized in a manner that pinpoints the most recent trends and the future directions. Physicochemical and biological treatment systems seem to be the most favored options while biofuels, biodegradable composites and biosorbents production paints a bright picture of the current and future bio-based products. Engineered microbes seem to tackle the problem of bioconversion of substrates that are otherwise non convertible by conventional wild strains. Although the main challenge facing LCW utilization is the high costs involved in treatment and production processes, some recent affordable processes with promising results have been proposed. Future trends are being directed to nanobiotechnology and genetic engineering for improved processes and products. The paper presents state of the art review of the dual advantage of handling LCW for cleaner environment and production of renewable bio-products.

232 citations


Cites background from "Fermentative production and kinetic..."

  • ...…production from LCW has been extensively studied (Jecu, 2000; Emtiazi and Nahvi, 2000; El-hawary and Mostafa, 2001; Ögel et al., 2001; Raj and Singh, 2001; Ojumu et al., 2003; Wen et al., 2005; Muthuvelayudham and Viruthagiri, 2006; Pothiraj et al., 2006; Daroit et al., 2007; Gao et al., 2008)....

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Journal ArticleDOI
TL;DR: Filamentous fungi Trichoderma reesei are considered to be one of the most efficient hyper producers of cellulase that is used in industry and were carried out using high concentration of cellulose to substitute glucose with the aim to improve cellulase production while trying to reduce production costs.

219 citations

Journal Article
TL;DR: The production of cellulolytic enzymes by a local isolate of A. heteromorphus on lignocelluloses in submerged fermentation is reported, indicating potential to reduce use of fossil fuels and reduce environmental pollution in India.
Abstract: enzyme production and meeting the increase in awareness on energy conservation and recycling. The conversion of cellulosic mass to fermentable sugars through biocatalyst cellulase derived from cellulolytic organisms has been suggested as a feasible process and offers potential to reduce use of fossil fuels and reduce environmental pollution (5), (6). Cellulase production by different organisms in submerged state fermentation has received more attention and is found to be cost-prohibitive because of high cost of process engineering. India is an agricultural country and wheat is one of the most important agricultural crop in India. Every year nearly 78.4 Million Tones wheat is produced. With the processesing of wheat grains a large amount of straw is produced which can be used as a substrate for ethanol production. Currently a most important application of cellulases and hemicellulases in the pulp and paper industry is the biobleaching of pulp, the production of dissolving pulp, the treatment of wastewater and the deinking of recycled waste paper. The potential of enzymatic treatments has been assessed and the processes have proved successful (7), (8). In the present study the production of cellulolytic enzymes by a local isolate of A. heteromorphus on lignocelluloses in submerged fermentation is reported.

84 citations


Cites result from "Fermentative production and kinetic..."

  • ...And CMCase activity was similar as reported by Muthuvelayudham and Viruthagir [15] using Sugarcane baggase as substrate by T. reesei 94.144....

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  • ...And CMCase activity was similar as reported by Muthuvelayudham and Viruthagir [15] using Sugarcane baggase as substrate by T....

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Journal ArticleDOI
15 May 2014-Fuel
TL;DR: A mutant strain of Trichoderma asperellum RCK2011 was developed through UV-irradiation for enhanced cellulase production and lower catabolite repression and the production of FPase, CMCase and β-glucosidase was optimized under solid state fermentation.

79 citations

Journal ArticleDOI
TL;DR: Proteomic analyses and enzymology studies are combined to show that sequential and submerged cultivation methods differently influence both titers and secretion profile of key enzymes required for the hydrolysis of sugarcane bagasse.

71 citations


Cites background from "Fermentative production and kinetic..."

  • ...21), which act synergistically in the conversion of cellulose into glucose [35, 36]....

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References
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Journal ArticleDOI
TL;DR: The production of cellulose and hemicellulose-degrading enzymes by cultivation of Aspergillus niger, Botrytis cinerea, Penicillium brasilianum, Schizophyllum commune, and Trichoderma reesei Rut-C30 was studied to obtain an enzyme mixture optimal for enzymatic hydrolysis of wet-oxidised wheat straw.

126 citations


"Fermentative production and kinetic..." refers background or methods in this paper

  • ...The formation of reducing sugar was measured by DNS method (Thygesen et al., 2003)....

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  • ...Cellulose and hemi cellu-lose-degrading enzyme was produced on wet-oxidized wheat straw using various filamentous fungi (Thygesen et al., 2003)....

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Journal ArticleDOI
TL;DR: A mutant of the native fungus Trichoderma aureoviride, selected for its overproduction of extracellular cellulase and s-glucosidase (cellobiase) was obtained and showed enhanced production of fungal cell wall degrading enzymes: chitinases,s-1,3- glucanases and proteases.

124 citations


"Fermentative production and kinetic..." refers methods in this paper

  • ...0.1 ml of the culture supernatant was incubated with 0.5 ml of 0.05 M acetate buffer containing 2.5 mg cellobiose (Zaldivar et al., 2001)....

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Journal ArticleDOI
TL;DR: This is the first report of a successful catabolite repression insensitivity of cellulase production by a mutant of B. pumilus, and is particularly significant when compared to Trichoderma reesei a well-known cellulase producer, which is under control of end-product inhibition.
Abstract: Cellulose degrading organisms have been used for the conversion of cellulolytic materials into soluble sugars or solvents in several biotechnological and industrial applications. In this report, a mutant of Bacillus pumilus was obtained after chemical mutagenesis and screened for cellulase production. This mutant named BpCRI 6 was selected for its ability to produce cellulase under catabolite repression. Cellulase yield by BpCRI 6 was four times higher than that of the wild type under optimum growth conditions (pH 6.5, 25°C and Ca 2+ 1mM). In shaking flask cultures, production of cellulase by the wild type was completely repressed in the presence of 25 mM glucose, while BpCRI 6 strain still exhibited a residual cellulase production of 80 and 40% at 25 mM and 40 mM of glucose concentrations respectively. The mutant strain is stable and grows rapidly in liquid and solid media. Under conditions of catabolite repression (40 mM of glucose), the production of cellulase by this mutant is particularly significant when compared to Trichoderma reesei a well-known cellulase producer, which is under control of end-product inhibition. This is the first report of a successful catabolite repression insensitivity of cellulase production by a mutant of B. pumilus.

108 citations


"Fermentative production and kinetic..." refers background in this paper

  • ...Cellulase was identified as one of the key enzyme degrading cellulose (Kotchoni et al., 2003)....

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01 Jan 1999

87 citations


"Fermentative production and kinetic..." refers background in this paper

  • ...Biosynthesis of cellulase protein was found to be most expensive process (Solomon et al., 1997)....

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Journal ArticleDOI
TL;DR: The conversion of cellulose using these enzymes was higher than that obtained with logen or Celluclast cellulases using steam-pretreated spruce as substrate, and the hydrolytic capacity of the produced enzyme solutions was higher compared with commercially available enzyme preparations.
Abstract: Various techniques are available for the conversion of lignocellulosics to fuel ethanol. During the last decade processes based on enzymatic hydrolysis of cellulose have been investigated more extensively, showing good yield on both hardwood and softwood. The cellulase production of a filamentous fungi, Trichoderma reesei Rut C 30, was examined on carbon sources obtained after steam pretreatment of spruce. These materials were washed fibrous steam-pretreated spruce (SPS), and hemicellulose hydrolysate. The hemicellulose hydrolysate contained, besides water-soluble carbohydrates, lignin and sugar degradation products, which were formed during the pretreatment and proved to be inhibitory to microorganisms. Experiments were performed in a 4-L laboratory fermentor. The hydrolytic capacity of the produced enzyme solutions was compared with two commercially available enzyme preparations, Celluclast and Iogen Cellulase, on SPS, washed SPS, and Solka Floc cellulose powder. There was no significant difference among the different enzymes produced by T. reesei Rut C 30. However, the conversion of cellulose using these enzymes was higher than that obtained with Iogen or Celluclast cellulases using steam-pretreated spruce as substrate.

73 citations


"Fermentative production and kinetic..." refers background in this paper

  • ...The enzymatic degradation of waste cellulose by the fungal enzymes has been suggested as a feasible alternative for the conversion of lignocellulosics into fermentable sugars and fuel ethanol (Szengyel et al., 2000)....

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