Fermentative production and kinetics of cellulase protein on Trichoderma reesei using sugarcane bagasse and rice straw
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TLDR
This is the first attempt of combining the synthetic substrate (xylose, lactose) with natural substrate (sugarcane bagasse, rice straw) and the mixture of substrates produced the highest maximal enzyme activity on cellulose with xylose.Abstract:
Cellulase a multienzyme made up of several proteins finds extensive applications in food, fermentation and textile industries. Trichoderma reesei is an efficient producer of cellulase protein. The comparative study was made on various carbon sources on the production of cellulase using strains of T. reesei QM 9414, 97.177 and Tm3. Pretreatment of sugarcane bagasse and rice straw offers very digestible cellulose and potentially less inhibition. Cellulase production was enhanced by multiple carbon sources because of diauxic pattern of utilization of substrates. This is the first attempt of combining the synthetic substrate (xylose, lactose) with natural substrate (sugarcane bagasse, rice straw). The mixture of substrates produced the highest maximal enzyme activity on cellulose with xylose, cellulose with lactose, bagasse with xylose, bagasse with lactose, rice straw with xylose and rice straw with lactose. In addition Monod growth kinetics and Leudeking piret product formation kinetics were studied using T. reesei with optimized medium under optimized conditions of inoculum concentration, D.O. level, agitator speed, temperature and pH.read more
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Secretome analysis of Trichoderma reesei and Aspergillus niger cultivated by submerged and sequential fermentation processes: Enzyme production for sugarcane bagasse hydrolysis.
Camila Florencio,F. M. Cunha,Alberto C. Badino,Cristiane S. Farinas,Eduardo Ximenes,Michael R. Ladisch +5 more
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References
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Biodegradation of the cellulose from sugarcane bagasse by fungal cellulase biodegradación de celulosa de bagazo de caña de azúcar por hongos celulolíticos biodegradación de celulosa de bagazo de caña de azucre por fungos celulolíticos
TL;DR: In this article, the enzyme production by Aspergillus niger IZ 9 was evaluated in different carbon sources: glucose, non-treated bagasse, bagasse treated with 4% solution of sodium hydroxide, phosphoric acid and steam; and filter paper.
Journal ArticleDOI
Optimal control strategy for the enhanced production of cellulase enzyme using the new mutant Trichoderma reesei E-12
Sudip Kumar Rakshit,V. Sahai +1 more
TL;DR: An optimal profile for pH, which was found to be the critical environmental parameter, was determined using a rigorous mathematical optimization procedure and a 30% increase in enzyme activity and productivity was obtained using the optimal pH strategy as compared to the pH cycling strategy.