Fertility of ram spermatozoa frozen by the pellet method. I. Transport and viability of spermatozoa within the genital tract of the ewe.
About: This article is published in Reproduction.The article was published on 1970-08-01 and is currently open access. It has received 94 citations till now. The article focuses on the topics: Semen.
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TL;DR: Factors influencing the fertility of stored semen and methods used for improvement are discussed, and fertility results of long-term frozen stored ram semen are given.
662 citations
Cites background from "Fertility of ram spermatozoa frozen..."
...Lightfoot and Salamon, 1970a,b; Salamon and Lightfoot, 1970 ....
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TL;DR: Cervical insemination with frozen-thawed semen had a relatively limited application in sheep because of its low Lambing results after cervicalInsemination varied depending on the parameters examined in the freezing technology or at insemination, but were low in comparison with those obtainable with fresh diluted semen.
279 citations
TL;DR: At present satisfactory and reliable lambing results are only obtainable by using intrauterine insemination by laparoscopy, and the most effective method is to increase the depth of deposition of frozen-thawed semen into the cervical canal.
268 citations
TL;DR: It is demonstrated that impaired function of cryopreserved spermatozoa can be overcome by addition of SP, resulting in normal fertility after cervical AI, and furthermore, SP improved pregnancy rates after cervical.
Abstract: The effect of seminal plasma (SP) on the motility, capacitation status, penetration through cervical mucus and fertility of frozen–thawed ram spermatozoa was examined. In the presence of SP, motility of frozen–thawed spermatozoa was better (P<0.001) and there were more uncapacitated and less acrosome-reacted cells in comparison with controls (P<0.001). Frozen–thawed spermatozoa were also better able to penetrate cervical mucus after addition of SP. Addition of SP increased the percentage of ewes pregnant after insemination of frozen–thawed (39/94, 41.5% v. 51/92, 55.4%; P<0.05) but not fresh spermatozoa (34/55, 61.8% v. 42/58, 72.4% for 0 v. 30% SP in the resuspension medium). Moreover, SP improved pregnancy rates after cervical (14/50; 28% v. 25/49; 51%; P<0.05) but not intrauterine insemination (25/44; 56.8 v. 26/43; 60.5%) with frozen–thawed spermatozoa. In a second experiment, pregnancy rates were 30/45 (66.7%), 9/37 (24.3%) and 24/40 (60.0%) for ewes inseminated with frozen–thawed spermatozoa in the uterus (control), cervix without SP and cervix after supplementation with SP, respectively (P<0.01 for unsupplemented v. supplemented spermatozoa). These experiments demonstrate that impaired function of cryopreserved spermatozoa can be overcome by addition of SP, resulting in normal fertility after cervical AI.
136 citations
TL;DR: It is suggested that ewe breed may be a critical determinant of the potential for the exploitation of cervical insemination of frozen-thawed semen in sheep breeding programmes and pregnancy rate was significantly influenced by breed of ewe and inseminator.
104 citations