Ferulic acid (FA) abrogates ionizing radiation-induced oxidative damage in murine spleen.
28 Nov 2016-International Journal of Radiation Biology (Int J Radiat Biol)-Vol. 92, Iss: 12, pp 806-818
TL;DR: This is perhaps the first comprehensive study with a mechanistic viewpoint that FA can protect the spleen from ionizing radiation and prevent the activation of apoptotic signaling events in theSpleen by interfering with the free radical chain reaction and by scavenging superfluous ROS.
Abstract: Purpose: The spleen is a crucial organ manifesting immune functions. Thus, radiation-induced oxidative challenge is vulnerable for the spleen. Our major objective was to protect the spleen from radiation-induced anomalous situations and to identify the signaling pathways involved.Materials and methods: Swiss albino mice were treated with ferulic acid (FA) once in a day at a dose of 50 mg/kg body weight for 5 consecutive days before exposing them to single dose of 10 Gy irradiation. The ROS generation and MMP change were determined by flow cytometry. The expression of different signaling proteins was investigated by immunoblotting and immunocytochemistry.Results: FA pretreatment significantly prevented radiation-induced oxidative stress by downregulating TBARS formation and by upregulating SOD and catalase activity. FA scavenged ROS, prevented the alteration of MMP and downregulated the expression of stress marker Cdc42 and apoptotic markers p53, p21, Bax and PTEN. Cell cycle analysis showed DNA da...
Citations
More filters
TL;DR: The current review addresses its therapeutic effects under different pathophysiological conditions (eg. cancer, cardiomyopathy, skin disorders, brain disorders, viral infections, diabetes etc.).
113 citations
TL;DR: Combining network pharmacology with validation experimental results, the nourishing blood effect of DBD might be manifested by the dual mechanism of enhancing immunity and promoting hematopoiesis.
85 citations
TL;DR: FA can challenge the oxidative stress by inducing nuclear translocation of Nrf2, scavenging ROS, and activating NHEJ DNA repair process to overcome ROS mediated oxidative stress and DNA damage.
Abstract: The present study was aimed to evaluate the radioprotective effect of ferulic acid (FA), a naturally occurring plant flavonoid in terms of DNA damage and damage related alterations of repair pathways by gamma radiation. FA was administered at a dose of 50 mg/kg body weight for five consecutive days prior to exposing the swiss albino mice to a single dose of 10 Gy gamma radiation. Ionising radiation induces oxidative damage manifested by decreased expression of Cu, Zn-SOD (SOD stands for super oxide dismutase), Mn-SOD and catalase. Gamma radiation promulgated reactive oxygen species (ROS) mediated DNA damage and modified repair pathways. ROS enhanced nuclear translocation of p53, activated ATM (ataxia telangiectasia-mutated protein), increased expression of GADD45a (growth arrest and DNA-damage-inducible protein) gene and inactivated Non homologous end joining (NHEJ) repair pathway. The comet formation in irradiated mice peripheral blood mononuclear cells (PBMC) reiterated the DNA damage in IR expo...
45 citations
TL;DR: It is found that ferulic acid reduced oxidative stress in the lungs by testing malondialdehyde level, myeloperoxidase level, and total anti-oxidant capacity, and it is proved that FA inactivated multiple mitogen-activated protein kinase signaling pathways in the lung.
Abstract: Acute respiratory distress syndrome (ARDS) is a fatal clinical condition that can be caused by pulmonary and non-pulmonary diseases. Oxidative stress and inflammation play key roles in the developm...
37 citations
TL;DR: It is found that Ferulic acid significantly reduced blood pressure, urine volume, and urinary protein level in rats with PE and suggested FA as a potential candidate for the treatment of various disorders including L-NAME induced preeclampsia in rats through decreasing placental inflammation and apoptosis.
Abstract: Preeclampsia (PE) occurs specifically during pregnancy characterized by new-onset hypertension The pathogenesis of PE was complicated, and inflammation may be central to the pathogenesis of PE Ferulic acid (FA) is recognized to prevent cell damage and apoptosis induced by oxidative stress and inflammation In our study, we used NG-nitro-l-arginine methyl ester (L-NAME)-induced rat model of PE to investigate whether FA improved PE and its possible mechanism We found that FA significantly reduced blood pressure, urine volume, and urinary protein level in rats with PE Meanwhile, FA decreased L-NAME induced higher expression of circulating TNF-α、IL-6、IL-1β and PlGF, it reduced placental TNF-α and NF-κB p65 Furthermore, FA rescued L-NAME induced decreasing expression of IL-4 and IL-10 expression in the circulation and placenta of rats FA also ameliorated placental apoptosis in L-NAME induced rats by increasing Bcl-2 whereas decreasing Bax expression in placenta It suggested FA as a potential candidate for the treatment of various disorders including L-NAME induced preeclampsia in rats through decreasing placental inflammation and apoptosis
28 citations
References
More filters
Journal Article•
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
289,852 citations
"Ferulic acid (FA) abrogates ionizin..." refers methods in this paper
...Protein concentration was determined by following the protocol of Lowry et al. (1951)....
[...]
TL;DR: The autoxidation of pyrogallol was investigated in the presence of EDTA in the pH range 7.9–10.6, indicating an almost total dependence on the participation of the superoxide anion radical, O2·−, in the reaction.
Abstract: The autoxidation of pyrogallol was investigated in the presence of EDTA in the pH range 7.9–10.6.
The rate of autoxidation increases with increasing pH. At pH 7.9 the reaction is inhibited to 99% by superoxide dismutase, indicating an almost total dependence on the participation of the superoxide anion radical, O2·−, in the reaction. Up to pH 9.1 the reaction is still inhibited to over 90% by superoxide dismutase, but at higher alkalinity, O2·− -independent mechanisms rapidly become dominant.
Catalase has no effect on the autoxidation but decreases the oxygen consumption by half, showing that H2O2 is the stable product of oxygen and that H2O2 is not involved in the autoxidation mechanism.
A simple and rapid method for the assay of superoxide dismutase is described, based on the ability of the enzyme to inhibit the autoxidation of pyrogallol.
A plausible explanation is given for the non-competitive part of the inhibition of catechol O-methyltransferase brought about by pyrogallol.
9,030 citations
"Ferulic acid (FA) abrogates ionizin..." refers methods in this paper
...Determination of superoxide dismutase activity SOD activity was determined by pyrogallol autoxidation method (Marklund and Marklund 1974) with slight modification....
[...]
...SOD activity was determined by pyrogallol autoxidation method (Marklund and Marklund 1974) with slight modification....
[...]
TL;DR: The activities of some glutathione-metabolizing enzymes were observed to be 5-to 60-fold lower in lung tissue than in the liver, and that phenobarbital nor methylcholanthrene had a significant effect on the levels of reduced glutATHione in lung and liver.
3,842 citations
"Ferulic acid (FA) abrogates ionizin..." refers background in this paper
...The thiol group of GSH reacts with DTNB formed a yellow complex, whose absorbance was read at 412 nm (Moron et al. 1979)....
[...]
TL;DR: Data indicate that JC‐1 is a reliable probe for analyzing ΔΨ changes with flow cytometry, while the others show a lower sensitivity (R123), or a non‐coherent behaviour, due to a high sensitivity to changes in plasmamembrane potential [DiOC6(3].
1,003 citations
"Ferulic acid (FA) abrogates ionizin..." refers methods in this paper
...JC1 was used to detect the change of Wm depolarization in the early stages of apoptosis (Salvioli et al. 1997)....
[...]
TL;DR: The latest studies strongly suggest that dietary polyphenols can stimulate antioxidant transcription and detoxification defense systems through AREs present in the promoter regions of many of the genes inducible by oxidative and chemical stress.
Abstract: Polyphenols are wide variety of compounds that occur in fruits and vegetables, wine, tea, extra virgin olive oil, chocolate and other cocoa products. Several polyphenols have been demonstrated to have clear antioxidant properties in vitro, and many of their biological actions have been attributed to their intrinsic reducing capabilities. However, this concept appears now to be a simplistic way to conceive their activity. Evidence is indeed accumulating that polyphenols might exert several other specific biological effects that are as yet poorly understood. In this article we review the most recent data on the subject and describe the additional functions that polyphenols can have in biological systems, focusing on their effects on glutathione and its related enzymes. Experimental data indicate that polyhenols may offer an indirect protection by activating endogenous defense systems. Several lines of evidence suggest a tight connection between exogenous and endogenous antioxidants that appear to act in a coordinated fashion. It is reasonable to hypothesize that this is achieved, at least in part, through antioxidant responsive elements (AREs) present in the promoter regions of many of the genes inducible by oxidative and chemical stress. The latest studies strongly suggest that dietary polyphenols can stimulate antioxidant transcription and detoxification defense systems through ARE.
999 citations
"Ferulic acid (FA) abrogates ionizin..." refers background in this paper
...Many research studies had already shown that antioxidants had an ability to activate the Nrf2 transcription factor against oxidative burst (Masella et al. 2005)....
[...]