Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
Citations
1,706 citations
Cites background from "Fibroblast Adaptation and Stiffness..."
...The magnitude of cellular contractility reflects the cell type and stat...
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1,073 citations
677 citations
Cites background from "Fibroblast Adaptation and Stiffness..."
...When cells generate traction forces higher than what the substrate can withstand, the matrix may yield and cells could adapt by decreasing the traction force, integrin binding and their own stiffness [16,17], which modulate cell spreading and thus differentiation [18,19]....
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References
12,204 citations
"Fibroblast Adaptation and Stiffness..." refers background or methods in this paper
...(A) Rhodamine-phalloidin staining of the F-actin of fixed fibroblasts on a 1-kPa gel (1), 5-kPa gel ( 2 ), 10-kPa gel (3), and glass (4)....
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...For example, mesenchymal stem cells can be differentiated into neurogenic, myogenic, or osteogenic cell types by varying the magnitude of matrix stiffness to mimic that of the native tissue ( 2 )....
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5,889 citations
"Fibroblast Adaptation and Stiffness..." refers background or methods in this paper
...These responses are cell-type dependent in that the effective range of substrate rigidity depends on the tissue type from which the cells are derived (4)....
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...(A) Rhodamine-phalloidin staining of the F-actin of fixed fibroblasts on a 1-kPa gel (1), 5-kPa gel (2), 10-kPa gel (3), and glass (4)....
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3,553 citations
"Fibroblast Adaptation and Stiffness..." refers background in this paper
...Stimulated contractility leads to an increase in the stress applied to cellular substrates (7,11), which has been shown to regulate the activity of small GTPases and the formation of focal adhesions (10,12)....
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...Cells grown on stiff substrates assemble actin stress fibers (5), exhibit a more spread phenotype (3), upregulate the expression of integrins (6), modify the properties and composition of their substrate adhesions (3,7,8), and activate signaling pathways characteristic of contractility (7,9,10)....
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...The model also predicts that the stress between a fully spread cell and the gel will increase with gel stiffness, as was recently shown by traction force experiments (7,47)....
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...(7,8,12,24), and these changes in turn can alter the assembly state of the cytoskeleton and the tension imposed on it by activated myosins or other motors....
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3,189 citations
"Fibroblast Adaptation and Stiffness..." refers background in this paper
...Additionally, if stiffer substrates allow more cell deformation than softer ones, as our model predicts, cells will tend to spread and migrate into stiffer regions, a phenomenon known as durotaxis that has been observed experimentally (19)....
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3,013 citations
"Fibroblast Adaptation and Stiffness..." refers background or methods in this paper
...Cells grown on stiff substrates assemble actin stress fibers (5), exhibit a more spread phenotype (3), upregulate the expression of integrins (6), modify the properties and composition of their substrate adhesions (3,7,8), and activate signaling pathways characteristic of contractility (7,9,10)....
[...]
...(A) Rhodamine-phalloidin staining of the F-actin of fixed fibroblasts on a 1-kPa gel (1), 5-kPa gel (2), 10-kPa gel (3), and glass (4)....
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