Fibroplasia after polypropylene mesh implantation for abdominal wall hernia repair in rats
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TLDR
Collagen was first seen on the 3rd day post-implantation, with a higher percentage of type I collagen at the last observational time point, and the prolonged healing inflammatory response and the persistence of chronic inflammation surrounding to the mesh did not affect the length of time required for fibroplasia.Abstract:
Purpose: This study assessed the collagen deposition and correlated it with local inflammatory responses to evaluate the length of time required for fibroplasia when polypropylene meshes are used to repair incisional abdominal wall hernias in rats. Methods: Thirty-six male Wistar rats underwent longitudinal resection of a peritoneal and musculoaponeurotic tissue segment (3x2 cm) of the abdominal wall followed by defect reconstruction with polypropylene mesh bridging over aponeurosis. The animals were divided into 6 groups according to the time points for the analysis of fibroplasia: 1, 2, 3, 7, 21 and 30 days post-implantation. Animals were sacrificed at each time point, and the site where the polypropylene mesh was implanted was evaluated histologically to assess inflammatory response and percentage of collagen using computer-assisted videomorphometry. Results: Total collagen was found at the mesh site on the 3rd day post-implantation, and increased progressively on all subsequent days up to the 21st day, when it reached its highest percentage (p 0.001). Type I collagen was first found between the 7th and 21st days; it reached its highest percentage on the 21st day and then remained stable until the 30th day. The type I to type III collagen ratio increased significantly and progressively up to the 30th day (p<0.001). Neutrophils were found at the mesh site from the 1st to the 21st day post-implantation. Macrophages, giant cells and lymphocytes were seen on the 2nd day. Thirty days after mesh implantation, neutrophils disappeared, but the percentages of macrophages, giant cells and lymphocytes remained stable (p<0.001). Conclusions: This study showed that total collagen was first seen on the 3rd day post-implantation, with a higher percentage of type I collagen at the last observational time point. The prolonged healing inflammatory response and the persistence of chronic inflammation surrounding to the mesh did not affect the length of time required for fibroplasia.read more
Citations
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Journal ArticleDOI
Mesh implants: An overview of crucial mesh parameters
TL;DR: Evaluating crucial mesh parameters to facilitate selection of the most appropriate mesh implant, considering raw materials, mesh composition, structure parameters and mechanical parameters found that mechanical properties of meshes, such as anisotropy/ isotropy, elasticity and tensile strength, are crucial parameters for predicting mesh performance after implantation.
Journal ArticleDOI
Experimental study comparing meshes made of polypropylene, polypropylene + polyglactin and polypropylene + titanium: inflammatory cytokines, histological changes and morphometric analysis of collagen
C. G. Pereira-lucena,Ricardo Artigiani-Neto,Gaspar de Jesus Lopes-Filho,C. V. G. Frazao,Alberto Goldenberg,Delcio Matos,Marcelo Moura Linhares +6 more
TL;DR: There were no significant differences in cytokine levels between the groups in the present study, but in the animals with the polypropylene + polyglactin mesh implant there was the most intense inflammatory process with lower tissue maturation and collagen deposition on the 40th postoperative day.
Journal ArticleDOI
Comparative study of shrinkage, inflammatory response and fibroplasia in heavyweight and lightweight meshes
TL;DR: It is concluded that LW-PP showed more shrinkage than HW-PP mesh at 7 and 90 days, despite the fact that HW- PP presented more lately foreign body reaction.
Journal ArticleDOI
Characterisation of the cellular infiltrate in the foreign body granuloma of textile meshes with its impact on collagen deposition
TL;DR: The high expression of at least four markers requires co-expression of different surface markers and thus confirms the existence of multiple transition forms instead of dominance of just macrophages, and offers new options for interventions to attenuate the inflammatory reaction of mesh implants.
Journal ArticleDOI
Early and late postoperative inflammatory and collagen deposition responses in three different meshes: an experimental study in rats.
C. G. Pereira-lucena,R. Artigiani Neto,D. T. de Rezende,G. de J. Lopes-Filho,Delcio Matos,Marcelo Moura Linhares +5 more
TL;DR: The inflammatory response depends on the mesh density and also the mesh material with clinical implications, whereas the PP + PG mesh caused severe tissue inflammation with small elevation in these levels.
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TL;DR: Examination of tissue sections from 15 species of vertebrates suggests that staining with Sirius Red, when combined with enhancement of birefringency, may be considered specific for collagen.
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Long-term Complications Associated With Prosthetic Repair of Incisional Hernias
TL;DR: Polyester mesh should no longer be used for incisional hernia repair and the deleterious effect of polyester mesh on long-term complications was confirmed on multiple logistic regression.
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TL;DR: Better understanding of the complex wound-healing cascade helps the approach to wound healing and its possible failure and Manipulations of the involved immunologic features offer future therapeutic strategies.
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Classification of biomaterials and their related complications in abdominal wall hernia surgery
TL;DR: The mechanism of these problems with special emphasis on pore size, molecular permeation and shrinkage of biomaterials and their effects on infection, seroma formation, and recurrence of mesh repair of abdominal wall hernias are discussed.
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Understanding the role of immune regulation in wound healing
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TL;DR: The mechanisms by which the immune system regulates wound healing are reviewed, including the secretion of cytokines, lymphokines, and growth factors.