Fine mapping of autophagy-related proteins during autophagosome formation in Saccharomyces cerevisiae.
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...Finally, the isolation membrane, mediated by two ubiquitin-like ATG conjugation pathways, Atg12-Atg5 and Atg8/LC3 conjugation systems, forms a closed bilayer membrane structure, mature autophagosome with an inner and outer membrane [43] (Fig....
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...The WIPI1/2-ATG2 (Atg18-Atg2 in yeast) complex localizes to the expanding edge of the isolation membrane and plays a key role in the elongation and/or closure of the isolation membrane [43, 142] (Fig....
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References
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"Fine mapping of autophagy-related p..." refers methods in this paper
...Cells expressing C-terminally (1–26)GFP- or (2–36)mCherry-fused Atg/Vps proteins were generated using a PCR-based gene-modification method (Longtine et al., 1998)....
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"Fine mapping of autophagy-related p..." refers background in this paper
...Among them, 18 ATG genes are essential for autophagosome formation upon starvation (Mizushima et al., 2011; Nakatogawa et al., 2009)....
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2,057 citations
"Fine mapping of autophagy-related p..." refers background in this paper
...Core Atg proteins are responsible for the formation of Cvt vesicles and autophagosomes (Xie and Klionsky, 2007)....
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