Journal ArticleDOI
First trimester pregnancy decidual natural killer cells contain and spontaneously release high quantities of granulysin.
Danijela Veljković Vujaklija,Tamara Gulic,Sonja Sucic,Kinya Nagata,Kazuyuki Ogawa,Gordana Laškarin,Shigeru Saito,Herman Haller,Daniel Rukavina +8 more
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TLDR
First trimester pregnancy decidual natural killer cells contain and spontaneously release high quantities of granulysin, and these cells act as “ghost cells” to defend against infection.Abstract:
Citation Veljkovic Vujaklija D, Gulic T, Sucic S, Nagata K, Ogawa K, Laskarin G, Saito S, Haller H, Rukavina D. First trimester pregnancy decidual natural killer cells contain and spontaneously release high quantities of granulysin. Am J Reprod Immunol 2011; 66: 363–372
Problem Granulysin (GNLY) is a novel cytolytic protein lytic against a variety of tumor cells and microbes. The role of GNLY during pregnancy has not been extensively explored. The aim of this study is to examine GNLY expression and distribution in the first trimester pregnancy peripheral blood (PB) and decidua, the ability of decidual and PB natural killer (NK) cells to secrete GNLY spontaneously, and the role of antigen-presenting cells (APC) in the regulation of GNLY expression in decidual NK cells.
Method of study GNLY expression was analyzed using cell permeabilization method, flow cytometry, and immunohistochemistry. GNLY secretion by purified NK cells was detected by ELISA method.
Results GNLY is abundantly expressed at the maternal–fetal interface in the first trimester pregnancy. Decidual T lymphocytes express significantly higher levels of GNLY (58%) then PB T lymphocytes (11%). Over 85% of decidual CD56+ cells express GNLY and when cultured spontaneously release high quantities of GNLY. Decidual APC participate in the control of GNLY expression in CD56+ cells.
Conclusion Abundant expression of GNLY in the decidual immunocompetent cells and the capacity of decidual CD56+ cells to spontaneously secrete high quantities of GNLY point to important protective and immunomodulatory role that this molecule could play at the maternal–fetal interface.read more
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Uterine NK cells: active regulators at the maternal-fetal interface
Ashley Moffett,Francesco Colucci +1 more
TL;DR: Allorecognition of fetal placental cells by uNK cells is emerging as the key maternal-fetal immune mechanism that regulates placentation and any therapies aimed at suppressing NK cells must be viewed with caution.
Journal ArticleDOI
Uterine Natural Killer Cells: Functional Distinctions and Influence on Pregnancy in Humans and Mice
TL;DR: The biology of human and mouse uNK cells are compared and contrast in the broader context of the biology of innate lymphoid cells and with reference to peripheral NK cells to help investigators that are beginning to study tissue NK cells.
Journal ArticleDOI
Evidence for a Role for the Adaptive Immune Response in Human Term Parturition
Nardhy Gomez-Lopez,Rodrigo Vega-Sanchez,Marisol Castillo-Castrejon,Roberto Romero,Karen Cubeiro-Arreola,Felipe Vadillo-Ortega +5 more
TL;DR: The purpose of this study was to characterize the immunophenotype of choriodecidual leukocytes as well as the expression of inflammatory mediators in human spontaneous parturition at term.
Journal ArticleDOI
Decidual NK Cells Transfer Granulysin to Selectively Kill Bacteria in Trophoblasts
Ângela C. Crespo,Ângela C. Crespo,Ângela C. Crespo,Sachin Mulik,Farokh Dotiwala,James Ansara,Sumit Sen Santara,Kayleigh Ingersoll,Cristian Ovies,Caroline Junqueira,Caroline Junqueira,Tamara Tilburgs,Jack L. Strominger,Judy Lieberman +13 more
TL;DR: It is shown that human dNK cells highly express the antimicrobial peptide granulysin (GNLY) and selectively transfer it via nanotubes to extravillous trophoblasts to kill intracellular Listeria monocytogenes (Lm) without killing the trophoblast.
Journal ArticleDOI
Cytotoxic potential of decidual NK cells and CD8+ T cells awakened by infections
Ângela C. Crespo,Ângela C. Crespo,Anita van der Zwan,Anita van der Zwan,João Ramalho-Santos,Jack L. Strominger,Tamara Tilburgs +6 more
TL;DR: The focus of this review is to address the regulation of cytotoxicity of dNK and CD8+ dT, which is essential for maternal-fetal immune tolerance as well as recent evidence that both cell types can provide immunity to infections at the maternal- Fetal interface.
References
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Journal ArticleDOI
An Antimicrobial Activity of Cytolytic T Cells Mediated by Granulysin
Steffen Stenger,Dennis A. Hanson,Rachel Teitelbaum,Puneet Dewan,Kayvan Niazi,Christopher J. Froelich,Tomas Ganz,Sybille Thoma-Uszynski,Agustı́n Melián,Christian Bogdan,Steven A. Porcelli,Barry R. Bloom,Alan M. Krensky,Robert L. Modlin +13 more
TL;DR: The ability of CTLs to kill intracellular M. tuberculosis was dependent on the presence of granulysin in cytotoxic granules, defining a mechanism by which T cells directly contribute to immunity against intrACEllular pathogens.
Journal ArticleDOI
Granulysin is a key mediator for disseminated keratinocyte death in Stevens-Johnson syndrome and toxic epidermal necrolysis
Wen-Hung Chung,Wen-Hung Chung,Shuen-Iu Hung,Shuen-Iu Hung,Jui-Yung Yang,Shih-Chi Su,Shien-Ping Huang,Chun-Yu Wei,See-Wen Chin,Chien-Chun Chiou,Sung-Chao Chu,Hsin-Chun Ho,Chih-Hsun Yang,Chi-Fang Lu,Jer-Yuarn Wu,You-Di Liao,Yuan-Tsong Chen,Yuan-Tsong Chen +17 more
TL;DR: It is demonstrated that secretory granulysin is a key molecule responsible for the disseminated keratinocyte death in SJS-TEN and this work highlights a mechanism for CTL- or NK cell—mediated cytotoxicity that does not require direct cellular contact.
Journal ArticleDOI
CD3- leukocytes present in the human uterus during early placentation: phenotypic and morphologic characterization of the CD56++ population.
TL;DR: It is proposed that the CD56bright+ uterine leukocytes represent a distinctive, hormonally regulated subset possibly adapted to control human placentation.
Journal Article
Processing, subcellular localization, and function of 519 (granulysin), a human late T cell activation molecule with homology to small, lytic, granule proteins.
TL;DR: The processing, subcellular localization, and function of a T and NK cell-specific granule protein that shares homology with small, lytic granule-associated molecules is described and lytic activity suggests the involvement of 519 in CTL effector function.
Journal ArticleDOI
Granulysin, a T Cell Product, Kills Bacteria by Altering Membrane Permeability
William A. Ernst,Sybille Thoma-Uszynski,Rachel Teitelbaum,Christine J. Ko,Dennis A. Hanson,Carol Clayberger,Alan M. Krensky,Matthias Leippe,Barry R. Bloom,Tomas Ganz,Robert L. Modlin +10 more
TL;DR: The data suggest that the ability of granulysin to kill microbial pathogens is dependent on direct interaction with the microbial cell wall and/or membrane, leading to increased permeability and lysis.