Fish intestinal microbiome: diversity and symbiosis unravelled by metagenomics.
Citations
643 citations
481 citations
Cites methods from "Fish intestinal microbiome: diversi..."
...These have been discussed in detail in some recent reviews (Zhou et al., 2014; Tarnecki et al., 2017) Briefly, they include quantitative real-time PCR (qPCR), used for quantitative analysis of taxa; clone libraries for identification of microbiota composition; finger-printing methods such as temporal temperature gradient electrophoresis (TTGE) and denaturing gradient gel electrophoresis (DGGE), and fluorescent in situ hybridization (FISH) used to determine the abundance of particular taxa, total microbial levels and assess bacterial–host interactions at the mucosal brush border (Zhou et al....
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...These have been discussed in detail in some recent reviews (Zhou et al., 2014; Tarnecki et al., 2017) Briefly, they include quantitative real-time PCR (qPCR), used for quantitative analysis of taxa; clone libraries for identification of microbiota composition; finger-printing methods such as…...
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247 citations
Cites background from "Fish intestinal microbiome: diversi..."
...To date, most of the studies on gut microbiota have focused on mammals, in particular rodents, and in comparison, little is known about the host-microbe interactions in fish (5)....
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222 citations
References
9,017 citations
"Fish intestinal microbiome: diversi..." refers background or methods in this paper
...The first attempts to explore fish intestinal microbiology used culture-based methods that vastly underestimate the diversity of these communities, as <10% of bacteria can be isolated and cultured under laboratory conditions (Amann et al. 1995)....
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...Fluorescence in situ hybridization (FISH) uses fluorescent-label probes to directly observe microbes, with minimal processing, using fluorescence or confocal microscopy (Amann et al. 1995)....
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7,023 citations
"Fish intestinal microbiome: diversi..." refers background in this paper
...Numerous reviews address NGS sequencing technologies, including sample preparation, sequencing chemistry and pros and cons (Metzker 2010; Mardis 2013; Chiu and Miller 2016)....
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4,730 citations
"Fish intestinal microbiome: diversi..." refers background in this paper
...Discovering a core microbiome, that is, members of the microbial community present in all individuals of a species, has been a primary goal for many researchers interested in understanding gut microbial communities (Turnbaugh et al. 2007)....
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1,755 citations
912 citations
"Fish intestinal microbiome: diversi..." refers background in this paper
...The theory of a core gut microbiome has primarily been explored in humans and mammalian models (Tap et al. 2009; Turnbaugh and Gordon 2009), but many authors believe that the same concept applies to bony fish....
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...The theory of a core gut microbiome has primarily been explored in humans and mammalian models (Tap et al. 2009; Turnbaugh and Gordon 2009), but many authors believe the same concept applies to bony fish....
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