Fluorescein diacetate as a stain of metabolically active bacteria in soil [garden soil, sandy soil, sand dune soil, mull, soil from Pinus, Picea and Fagus forest, fluorescence technique, acridine orange staining, fluorescein isothiocyanate staining, plate counts]
Citations
1,176 citations
Cites background from "Fluorescein diacetate as a stain of..."
...Fluorescein diacetate (3',6'-diacetylfluorescein [FDA]) has been used to determine amounts of active fungi (14) and bacteria (1, 8) and to locate acetylesterases in living protist cells (10)....
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...In the present investigation, no attempt was made to relate the observed total FDA activity to the active microbial biomass determined by FDA microscopic techniques (8, 14)....
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...Earlier studies have shown that all fungi investigated (10, 14), most bacteria (8, 10), and some protozoa and algae (10) exhibit FDA hydrolytic activity....
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640 citations
488 citations
Cites background from "Fluorescein diacetate as a stain of..."
...Fluorescein diacetate [3 0,6 0-diacetylfluorescein (FDA)] can be used to measure microbial activity in soils (Brunius, 1980; Lundgren, 1981; Schnürer and Rosswall, 1982)....
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...The product of this enzymatic reaction is fluorescein, which can be visualized within cells by fluorescence microscopy (Lundgren, 1981). Fluorescein released in soil can also be measured by spectrophotometry (Swisher and Carroll, 1980; Schnürer and Rosswall, 1982). A search of Soil Biology & Biochemistry 38 (2006) 693–701...
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...5 pH range that Lundgren (1981) used in studying FDA as a stain for metabolically active bacteria in soil....
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...The product of this enzymatic reaction is fluorescein, which can be visualized within cells by fluorescence microscopy (Lundgren, 1981)....
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484 citations
Cites background from "Fluorescein diacetate as a stain of..."
...In a study on the use of FDA as a vital stain to detect metabolically active bacteria in soil, Lundgren (1981) noted that fewer organisms hydrolysed FDA if they were grown in laboratory medium....
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...Lundgren (1981) had previously noted the problem of autofluorescence when using FDA for bacteriological applications and the same difficulty was experienced in our laboratory....
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...For example, Lundgren (1981) reports that ¼20% of the isolates tested did not stain with FDA....
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476 citations
References
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"Fluorescein diacetate as a stain of..." refers background in this paper
...Since FDA is hydrolysed rather unspecifically (Rotman and Papermaster 1966) the reason is probably not as simple as a lack of suitable enzymes....
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...esterases), and hence the cells will glow in UV-light (Rotman and Papermaster 1966)....
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640 citations
488 citations
484 citations