Journal ArticleDOI
Fluorescence molecular tomography resolves protease activity in vivo
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TLDR
It is demonstrated that enzyme-activatable fluorochromes can be detected with high positional accuracy in deep tissues, that molecular specificities of different beacons towards enzymes can be resolved and that tomography of beacon activation is linearly related to enzyme concentration.Abstract:
Systematic efforts are under way to develop novel technologies that would allow molecular sensing in intact organisms in vivo Using near-infrared fluorescent molecular beacons and inversion techniques that take into account the diffuse nature of photon propagation in tissue, we were able to obtain three-dimensional in vivo images of a protease in orthopic gliomas We demonstrate that enzyme-activatable fluorochromes can be detected with high positional accuracy in deep tissues, that molecular specificities of different beacons towards enzymes can be resolved and that tomography of beacon activation is linearly related to enzyme concentration The tomographic imaging method offers a range of new capabilities for studying biological function; for example, identifying molecular-expression patterns by multispectral imaging or continuously monitoring the efficacy of therapeutic drugsread more
Citations
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Nanoshell-mediated near-infrared thermal therapy of tumors under magnetic resonance guidance
Leon R. Hirsch,R.J. Stafford,James A. Bankson,S.R. Sershen,Belinda Rivera,Roger E. Price,John D. Hazle,Nancy J. Halas,Jennifer L. West +8 more
TL;DR: In vivo studies under magnetic resonance guidance revealed that exposure to low doses of NIR light in solid tumors treated with metal nanoshells reached average maximum temperatures capable of inducing irreversible tissue damage, and found good correlation with histological findings.
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In vivo near-infrared fluorescence imaging
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Molecular imaging in living subjects: seeing fundamental biological processes in a new light
TL;DR: This article cites 228 articles, 79 of which can be accessed free at: service Email alerting click here top right corner of the article or Receive free email alerts when new articles cite this article sign up in the box at the Collections Topic.
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Imaging in the era of molecular oncology
TL;DR: Advances in experimental and clinical imaging are likely to improve how cancer is understood at a systems level and should enable doctors not only to locate tumours but also to assess the activity of the biological processes within these tumours and to provide 'on the spot' treatment.
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Shedding light onto live molecular targets
TL;DR: Optical sensing of specific molecular targets and pathways deep inside living mice has become possible as a result of a number of advances, which will provide new tools making it possible to understand more fully the functioning of protein networks, diagnose disease earlier and speed along drug discovery.
References
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Optical tomography in medical imaging
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In vivo imaging of tumors with protease-activated near-infrared fluorescent probes.
TL;DR: In vivo imaging showed a 12-fold increase in NIRF signal, allowing the detection of tumors with submillimeter-sized diameters, and this strategy can be used to detect such early stage tumors in vivo and to probe for specific enzyme activity.
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Concurrent MRI and diffuse optical tomography of breast after indocyanine green enhancement
TL;DR: It is found that DOT provides for localization and quantification of exogenous tissue chromophore concentrations and the use of ICG, an albumin bound absorbing dye in plasma, demonstrates the potential to differentiate disease based on the quantified enhancement of suspicious lesions.