Fluorescence Silica Nanoprobe as a Biomarker for Rapid Detection of Plant Pathogens
TL;DR: It is demonstrated that the fluorescence silica nanoprobe biomarker will have been potential for rapid diagnosis applications on plant diseases.
Abstract: Fluorescent silica nanoprobe as a biomarker for detection has attracted much attention in the field of nano-biotechnology recently but no further research applications using fluorescent silica nanoparticles (FSNP) combined with antibody molecules reported to detect pathogen detection. In this study, silica nanoparticles were prepared using the water-in-oil (w/o) microemulsion method. The silica nanoparticles were circular in diameter of 50 ± 4.2 nm. The organic dye, tris-2, 2' -bipyridyl dichlororuthenium (II) hexahydrate (Rubpy), could be incorporated efficiently into the core of silica nanoparticles. The fluorescence of Rubpy-doped silica nanoparticles was photostable using a collisional quenching fluorescence test. The Rubpy-doped silica nanoparticles were conjugated with the secondary antibody of goat anti-rabbit immunoglobulin G (IgG) and successfully detected plant pathogen such as Xanthomonas axonopodis pv. vesicatoria that causes bacterial spot disease in Solanaceae plant. These results demonstrated that the fluorescence silica nanoprobe biomarker will have been potential for rapid diagnosis applications on plant diseases.
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Cites background or methods from "Fluorescence Silica Nanoprobe as a ..."
...Nanoparticles have been utilized with other biological materials such as antibody for detecting Xanthomonas axonopodis that causes bacterial spot disease [65]....
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...Vesicatoria that cause bacterial spot diseases in Solanaceae plant [65]....
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...Different types of phytopathogens—phytobacteria, viruses and fungi—have been detected through nanoparticle based amperometric biosensors [65,78,79]....
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References
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TL;DR: Semiconductor nanocrystals prepared for use as fluorescent probes in biological staining and diagnostics have a narrow, tunable, symmetric emission spectrum and are photochemically stable.
Abstract: Semiconductor nanocrystals were prepared for use as fluorescent probes in biological staining and diagnostics. Compared with conventional fluorophores, the nanocrystals have a narrow, tunable, symmetric emission spectrum and are photochemically stable. The advantages of the broad, continuous excitation spectrum were demonstrated in a dual-emission, single-excitation labeling experiment on mouse fibroblasts. These nanocrystal probes are thus complementary and in some cases may be superior to existing fluorophores.
8,542 citations
TL;DR: Efficient collection and detection of fluorescence coupled with careful minimization of background from impurities and Raman scattering now enable routine optical microscopy and study of single molecules in complex condensed matter environments.
Abstract: Efficient collection and detection of fluorescence coupled with careful minimization of background from impurities and Raman scattering now enable routine optical microscopy and study of single molecules in complex condensed matter environments. This ultimate method for unraveling ensemble averages leads to the observation of new effects and to direct measurements of stochastic fluctuations. Experiments at cryogenic temperatures open new directions in molecular spectroscopy, quantum optics, and solid-state dynamics. Room-temperature investigations apply several techniques (polarization microscopy, single-molecule imaging, emission time dependence, energy transfer, lifetime studies, and the like) to a growing array of biophysical problems where new insight may be gained from direct observations of hidden static and dynamic inhomogeneity.
1,053 citations
TL;DR: In this paper, a water-in-oil microemulsion method has been applied for the preparation of silica-coated iron oxide nanoparticles, and their effects on the particle size, crystallinity, and the magnetic properties have been studied.
Abstract: A water-in-oil microemulsion method has been applied for the preparation of silica-coated iron oxide nanoparticles. Three different nonionic surfactants (Triton X-100, Igepal CO-520, and Brij-97) have been used for the preparation of microemulsions, and their effects on the particle size, crystallinity, and the magnetic properties have been studied. The iron oxide nanoparticles are formed by the coprecipitation reaction of ferrous and ferric salts with inorganic bases. A strong base, NaOH, and a comparatively mild base, NH4OH, have been used in each surfactant to observe whether the basicity has some influence on the crystallization process during particle formation. Transmission electron microscopy, X-ray electron diffraction, and superconducting quantum interference device magnetometry have been employed to study both uncoated and silica-coated iron oxide nanoparticles. All these particles show magnetic behavior close to that of superparamagnetic materials. By use of this method, magnetic nanoparticles ...
772 citations
"Fluorescence Silica Nanoprobe as a ..." refers background in this paper
...Recently, Santra et al. (2001) clearly demonstrated that luminophore-doped silica nanoparticles using the water-in-oil (W/O) microemulsion method have many advantages including high fluorescent intensity, photostability, simple operation for leukemia cell identification, and uniform and smooth…...
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TL;DR: A new molecular conjugation method has been developed to label biomolecules with optically stable metalorganic luminophores, such as tris(2,2'-bipyridyl)dichlororuthenium(II) hexahydrate (Rubpy), which are otherwise not possible for direct linking with the biomolecule.
Abstract: A new molecular conjugation method has been developed to label biomolecules with optically stable metalorganic luminophores, such as tris(2,2‘-bipyridyl)dichlororuthenium(II) hexahydrate (Rubpy), which are otherwise not possible for direct linking with the biomolecules. Unique biochemical properties of the biomolecule can, thus, be associated with photostable luminophores. This opens a general way to conjugate desired biomolecules using a sensitive signal transduction method. It also promotes the application of excellent luminescent materials, especially those based on photostable metalorganic luminophores, in biochemical analysis and biomolecular interaction studies. The conjugation method is based on uniform luminophore-doped silica (LDS) nanoparticles (63 ± 4 nm). These nanoparticles have been prepared using a water-in-oil (W/O) microemulsion method. The controlled hydrolysis of tetraethyl orthosilicate (TEOS) in W/O microemulsion leads to the formation of monodisperse LDS nanoparticles. The luminophor...
758 citations
"Fluorescence Silica Nanoprobe as a ..." refers background in this paper
...Recently, Santra et al. (2001) clearly demonstrated that luminophore-doped silica nanoparticles using the water-in-oil (W/O) microemulsion method have many advantages including high fluorescent intensity, photostability, simple operation for leukemia cell identification, and uniform and smooth…...
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TL;DR: In this article, stable dispersions of colloidal colloidal silica spheres containing a dye or fluorophore have been synthesized according to a general procedure and dispersed in polar and apolar liquids.
Abstract: Stable dispersions of monodisperse colloidal silica spheres containing a dye or fluorophore have been synthesized according to a general procedure and dispersed in polar and apolar liquids. The procedure consists of the coupling of the dye to a silane coupling agent, (3++minopropyl)triethoxysilane, and the controllable incorporation of the reaction product into the silica sphere. The silica spheres are prepared from tetraethoxysilane in mixtures of ammonia, water, and ethanol. The composition of the silica spheres can be controlled in such a way that the organic groups can be placed on the surface, in a thin shell inside the particle or distributed through the volume of an inner core. Fluorescein isothiocyanate was used to make easily bleachable, fluorescent silica spheres. Hydrophilic charge stabilized and organophilic sterically stabilized 1-octadecanol-coated dyed silica systems were synthesized and dispersed in several solvents. All the particles were characterized after the several reaction steps by static and dynamic light scattering and transmission electron microscopy. The fluorescent spheres were further characterized by fluorescence spectroscopy and confocal scanning laser fluorescence microscopy. Great effort was taken to prepare monodisperse dispersions free of clusters of particles. Such model dispersions are required for (scattering) studies of interparticle interactions in (concentrated) systems. Therefore, the several steps of the synthesis and optical characterization are described in detail.
707 citations
"Fluorescence Silica Nanoprobe as a ..." refers result in this paper
...The FSNP diameter in this study was much smaller than that (140 nm) of the nanoparticles described by Blaaderen and Vrij, 1992 [22]....
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