Proceedings ArticleDOI
Fluorescent molecular probes based on excited state prototropism in lipid bilayer membrane
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TLDR
The recent work using 1-naphthol as an ESPT fluorescent molecular probe has shown that the incorporation of monomeric bile salt molecules into lipid bilayer membranes composed from dipalmitoylphosphatidylcholine and dimyristoy lphosph atidyl choline induce appreciable wetting of the bilayer up to the hydrocarbon core region, even at very low concentrations of the bile salts.Abstract:
Excited state prototropism (ESPT) is observed in molecules having one or more ionizable protons, whose proton
transfer efficiency is different in ground and excited states. The interaction of various ESPT molecules like naphthols
and intramolecular ESPT (ESIPT) molecules like hydroxyflavones etc. with different microheterogeneous media have
been studied in detail and excited state prototropism as a probe concept has been gaining ground. The fluorescence of
different prototropic forms of such molecules, on partitioning to an organized medium like lipid bilayer membrane,
often show sensitive response to the local environment with respect to the local structure, physical properties and
dynamics. Our recent work using 1-naphthol as an ESPT fluorescent molecular probe has shown that the incorporation
of monomeric bile salt molecules into lipid bilayer membranes composed from dipalmitoylphosphatidylcholine (DPPC,
a lung surfactant) and dimyristoylphosphatidylcholine (DMPC), in solid gel and liquid crystalline phases, induce
appreciable wetting of the bilayer up to the hydrocarbon core region, even at very low (≤ 1 mM) concentrations of the
bile salts. The incorporation and location of fisetin, an ESIPT molecule having antioxidant properties, in lipid bilayer
membrane has been sensitively monitored from its intrinsic fluorescence behaviour.read more
Citations
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Journal ArticleDOI
Photophysical behavior of 8-anilino-1-naphthalenesulfonate in vesicles of pulmonary surfactant dipalmitoylphosphatidylcholine (DPPC) and its sensitivity toward the bile salt-vesicle interaction.
TL;DR: Lower-temperature shift in the phase transition of DPPC bilayer indicates that fluorescence anisotropy of ANS is sensitive enough to the bile salt induced perturbation in the packed acyl chains of DP PC bilayer and modification in the membrane fluidity.
References
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Journal ArticleDOI
Berberine alkaloid as a sensitive fluorescent probe for bile salt aggregates.
Mónika Megyesi,László Biczók +1 more
TL;DR: The aggregate disrupting power of cosolvents decreased in the series of dimethylformamide, acetonitrile, formamide, and methanol, which enhanced the water accessibility of berberine bound to aggregates and diminished the number of secondary aggregates.
Journal ArticleDOI
Effect of ionic and neutral surfactants on the properties of phospholipid vesicles: investigation using fluorescent probes
J. Sujatha,Ashok Kumar Mishra +1 more
TL;DR: In this paper, the effect of triton X-100, sodium dodecyl sulphate, and cetyl trimethyl ammonium bromide on the physical properties of dimytistoyl phosphatidyl choline liposome was studied in the sublytic concentration range of the detergent, using the different fluorescence probes 8-anilino-1-naphthalene sulphonate, 1,6-diphenylhexatriene and 1 -naphthol.
Journal ArticleDOI
Effect of submicellar concentrations of conjugated and unconjugated bile salts on the lipid bilayer membrane.
TL;DR: Steady-state and time-resolved fluorescence of the two excited-state prototropic forms of 1-naphthol indicate that submicellar bile salt concentration induces hydration of the lipid bilayer membrane into the core region.
Journal ArticleDOI
1-Naphthol as a Sensitive Fluorescent Molecular Probe for Monitoring the Interaction of Submicellar Concentration of Bile Salt with a Bilayer Membrane of DPPC, a Lung Surfactant
TL;DR: Steady-state and time-resolved fluorescence of the two excited state prototropic forms of 1-naphthol indicate that the incorporation of monomeric bile salt molecules in the lipid bilayer membrane induces appreciable wetting of the bilayer up to the hydrocarbon core region, even at very low (≤1 mM) concentrations of the bile salts.
Journal ArticleDOI
Effect of sodium deoxycholate and sodium cholate on DPPC vesicles: A fluorescence anisotropy study with diphenylhexatriene
TL;DR: It was found that the variation of rss is sensitive enough to monitor different stages of interaction of bile salts with DPPC vesicles and showed that the bile salt-induced changes of vesicle structure were strongly dependent on the concentration of the biles salt and not on the molar ratio of lipid and bile Salt.
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1-Naphthol as a Sensitive Fluorescent Molecular Probe for Monitoring the Interaction of Submicellar Concentration of Bile Salt with a Bilayer Membrane of DPPC, a Lung Surfactant
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