Forage Fiber Analysis (Apparatus, reagents, procedures and some applications)
01 Jan 1970-
About: The article was published on 1970-01-01 and is currently open access. It has received 5332 citations till now. The article focuses on the topics: Fiber & Forage.
Citations
More filters
TL;DR: In addition to NDF, new improved methods for total dietary fiber and nonstarch polysaccharides including pectin and beta-glucans now are available and are also of interest in rumen fermentation.
23,302 citations
TL;DR: These mutants—the ‘Keio collection’—provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome‐wide testing of mutational effects in a common strain background, E. coli K‐12 BW25113.
Abstract: We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosomal genes and primers designed to create in-frame deletions upon excision of the resistance cassette. Of 4288 genes targeted, mutants were obtained for 3985. To alleviate problems encountered in high-throughput studies, two independent mutants were saved for every deleted gene. These mutants-the 'Keio collection'-provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome-wide testing of mutational effects in a common strain background, E. coli K-12 BW25113. We were unable to disrupt 303 genes, including 37 of unknown function, which are candidates for essential genes. Distribution is being handled via GenoBase (http://ecoli.aist-nara.ac.jp/).
7,428 citations
TL;DR: The Cornell Net Carbohydrate Protein Model (Chalupa et al., 1991) has developed the need for uniform procedures to partition feed nitrogen into A, B, and C fractions (Pichard and Van Soest, 1977) as mentioned in this paper.
2,282 citations
TL;DR: Interspecific patterns of defense mechanisms are discussed in terms of current theories of plant apparency, and an alternative model for the evolution of plant defenses is presented.
Abstract: Rates of herbivory and defensive characteristics of young and mature leaves were measured for saplings of 46 canopy tree species in a lowland tropical rain forest (Barro Colorado Island, Panama). Grazing rates were determined in the field for sample periods in the early wet, late wet, and dry seasons. Leaf properties such as pubescence, toughness, water, protein, fiber, and phenolic contents explained over 70W% of the variation among plant species in the rates of herbivory on mature leaves. Leaf toughness was most highly correlated with levels of herbivory, followed by fiber content and nutritive value. Phenol content and phenol: protein ratios were not significantly correlated with damage. Mature leaves of gap-colonizing species were grazed six times more rapidly than leaves of shade- tolerant species. Gap-colonizers have less tough leaves, lower concentrations of fiber and phenolics, higher levels of nitrogen and water, shorter leaf lifetimes, and faster growth rates than do shade- tolerant species. Gap-colonizers did not escape discovery by herbivores to any greater extent than shade-tolerant species, as measured by the spatial distribution of plants or by the intraspecific dis- tribution of herbivore damage under natural or experimentally manipulated conditions. In 70W% of the species, young leaves suffered higher damage levels than mature leaves. Although young leaves are more nutritious and less tough and fibrous, they have two to three times the con- centrations of phenols. The temporal appearance of young leaves was not correlated with the distri- bution of herbivory among individuals of a species. Interspecific patterns of defense mechanisms are discussed in terms of current theories of plant apparency, and an alternative model for the evolution of plant defenses is presented.
1,523 citations
TL;DR: The system is based on NDF as the measure of total chemical fiber in feeds and Adjustments for the effectiveness of NDF in maintaining milk fat production and optimizing ruminal fermentation are based on the particle size and inherent characteristics ofNDF that affect chewing activity, ruminal pH, and milkfat production.
1,269 citations