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Journal ArticleDOI

Forskolin synthesis in in vitro cultures of Coleus forskohlii Briq transformed with Agrobacterium tumefaciens.

01 May 1996-Plant Cell Reports (Springer-Verlag)-Vol. 15, Iss: 9, pp 691-694
TL;DR: F Forskolin synthesis and accumulation in tumorous C. forskohlii cultures may permit the elucidation of diterpene metabolism in this species.
Abstract: Agrobacterium tumefaciens mediated tumor tissue and shooty teratomas of Coleus forskohlii were cultured in vitro. Forskolin was detected in tumorous callus (0.002%), rhizogenic callus (0.011%) and root cultures (0.014%), but not in shooty teratomas. Forskolin synthesis and accumulation in tumorous C. forskohlii cultures may permit the elucidation of diterpene metabolism in this species.
Citations
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Journal ArticleDOI
TL;DR: It is suggested that the use of the root endophyte fungus P. indica in sustainable agriculture will enhance the medicinally important chemical production.
Abstract: This study was undertaken to investigate the influence of plant probiotic fungus Piriformospora indica on the medicinal plant C. forskohlii. Interaction of the C. forskohlii with the root endophyte P. indica under field conditions, results in an overall increase in aerial biomass, chlorophyll contents and phosphorus acquisition. The fungus also promoted inflorescence development, consequently the amount of p-cymene in the inflorescence increased. Growth of the root thickness was reduced in P. indica treated plants as they became fibrous, but developed more lateral roots. Because of the smaller root biomass, the content of forskolin was decreased. The symbiotic interaction of C. forskohlii with P. indica under field conditions promoted biomass production of the aerial parts of the plant including flower development. The plant aerial parts are important source of metabolites for medicinal application. Therefore we suggest that the use of the root endophyte fungus P. indica in sustainable agriculture will enhance the medicinally important chemical production.

136 citations


Cites methods from "Forskolin synthesis in in vitro cul..."

  • ...The forskolin content of the underground parts of the plants was determined by HPLC.78 For this purpose, samples were prepared from six months old P.indica colonized and non colonized plants....

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  • ...The forskolin content of the underground parts of the plants was determined by HPLC.(78) For this purpose, samples were prepared from six months old P....

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  • ...and forskolin contents was measured as described by Mukherjee et al.(78)...

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Journal ArticleDOI
TL;DR: Suspension cultures derived from gall calli which were obtained following infection with Agrobacterium tumefaciens were established in Coleus forskohlii and showed continuous and stable production of forskolin.

46 citations

Journal ArticleDOI
TL;DR: Transformed organ cultures of Withania somnifera established following infection with wild type nopaline and octopine strains of Agrobacterium tumefaciens found shooty teratomas of altered phenotype spontaneously developed, finding the main difference was found in the nature of the galls formed and in their subsequent morphological competence.

38 citations

Journal ArticleDOI
TL;DR: Transformed organ cultures of Artemisia annua were established following infection with two wild type nopaline strains of Agrobacterium tumefaciens: Parameters like explant type, strain type, age of the plant source for explants, affected the tumorigenesis frequency significantly.

30 citations

Journal ArticleDOI
TL;DR: The significance of the present micropropagation protocol of C. forskohlii is the formulation of growth regulators which effected very fast multiplication of the plant (time reduced to one-third of the hitherto known methods).
Abstract: A protocol has been developed that leads to the development of complete plantlets of Coleus forskohlii within 35–40 d by culturing stem tip explants in MS medium containing 0.57 μM indole-3-acetic acid and 0.46 μM kinetin through direct multiplication at the rate of 12.5 shoots per explant. About 100% shoots rooted and micropropagated plants were successfully established in soil after hardening with a high survival rate. The significance of the present micropropagation protocol of C. forskohlii is the formulation of growth regulators which effected very fast multiplication of the plant (time reduced to one-third of the hitherto known methods).

29 citations

References
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Journal ArticleDOI
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Abstract: In experiments with tobacco tissue cultured on White's modified medium (basal meditmi hi Tnhles 1 and 2) supplemenk'd with kiticthi and hidoleacctic acid, a slrikin^' fourlo (ive-told intTease iu yield was ohtaitu-d within a three to Tour week j^rowth period on addition of an aqtteotis exlrarl of tobacco leaves (Fi^'ures 1 and 2). Subse(iueutly it was found Ihiit this jnoniotiou oi' f^rowih was due mainly though nol entirely to inorj^auic rather than organic con.stitttenls in the extract. In the isolation of Rrowth factors from plant tissues and other sources inorj '̂anic salts are fre(|uently carried along with fhe organic fraclioits. When tissue cultures are used for bioassays, therefore, il is necessary lo lake into account increases in growth which may result from nutrient elements or other known constituents of the medium which may he present in the te.st materials. To minimize interference trom rontaminaitis of this type, an altempt has heen made to de\\eh)p a nieditmi with such adequate supplies of all re(iuired tnineral nutrients and cotntnott orgattic cottslitueitls that no apprecial»le change in growth rate or yield will result from the inlroduclion of additional amounts in the range ordinarily expected to be present in tnaterials to be assayed. As a point of referetice for this work some of the culture media in mc)st common current use will he cotisidered briefly. For ease of comparis4)n Iheir mineral compositions are listed in Tables 1 and 2. White's nutrient .solution, designed originally for excised root cultures, was based on Uspeuski and Uspetiskaia's medium for algae and Trelease and Trelease's micronutrieni solution. This medium also was employed successfully in the original cttltivation of callus from the tobacco Iiybrid Nicotiana gtauca x A', tanijadorffii, atitl as further modified by White in 194̂ ^ and by others it has been used for the

63,098 citations

Journal ArticleDOI
TL;DR: The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2, 4- d) has been designed.

9,342 citations

Journal ArticleDOI
01 Apr 1983-Cell
TL;DR: Comparison of T-DNA-encoded transcripts present in crown gall tumors showing teratoma-like growth (BT37) with those from an unorganized tumor line (W38C58) reveals that this difference in phenotype is accompanied by a difference in the expression of the T- DNA.

154 citations

Journal ArticleDOI
TL;DR: Agrobacterium tumefaciens strains, known to induce tobacco crown galls that spontaneously develop shoots, were used to induce galls on cultured shoots of a tetraploid potato cultivar and most of the tumour-derived shoots formed roots, did not produce opines and were indistinguishable from the parental plants on the basis of morphology and chromosome numbers.
Abstract: Agrobacterium tumefaciens strains, known to induce tobacco crown galls that spontaneously develop shoots, were used to induce galls on cultured shoots of a tetraploid potato cultivar (Solanum tuberosum cv. ‘Maris Bard’). Shoots also appeared spontaneously from the induced potato galls, although only after 2–4 months. The shoots were excised and cultured separately. Some of these frequently developed side-shoots from their axillary buds. They did not form roots and they produced opines, a strong indication that they were transformed and carried T-DNA. Grafts of the transformed plants were still able to develop tubers. Most of the tumour-derived shoots, however, formed roots, did not produce opines and were indistinguishable from the parental plants on the basis of morphology and chromosome numbers (48 chromosomes per cell). The results are discussed in relation to the origin of previously described variation among protoplast-derived potato plants and with respect to genetic engineering of tetraploid potato cultivars.

56 citations

Journal ArticleDOI
TL;DR: F Forskolin was identified by TLC and HPLC in 60 days old shoot differentiating culture, 30 days old micropropagated plants, and root organ suspension but not in rhizogenic callus.
Abstract: Shoot tip culture, root organ suspension, and callus culture producing roots were established from shoot tip, root tip, and hypocotyl segments, respectively, on Murashige and Skoog's and White's basal media with or without growth regulators. Forskolin was identified by TLC and HPLC in 60 days old shoot differentiating culture, 30 days old micropropagated plants, and root organ suspension but not in rhizogenic callus. Shoot differentiating culture established in the presence or absence of BAP revealed more or less similar amounts of forskolin as that of the micropropagated plants. Root organ suspension showed traces of forskolin production.

34 citations