Fresnel incoherent correlation holography with single camera shot
TL;DR: In this paper, the authors used a randomly multiplexed bifocal binary diffractive Fresnel zone lenses fabricated using electron beam lithography for super-resolution 3D imaging.
Abstract: Fresnel incoherent correlation holography (FINCH) is a self-interference based super-resolution three-dimensional imaging technique. FINCH in inline configuration requires an active phase modulator to record at least three phase-shifted camera shots to reconstruct objects without twin image and bias terms. In this study, FINCH is realized using a randomly multiplexed bifocal binary diffractive Fresnel zone lenses fabricated using electron beam lithography. The object space is calibrated by axially scanning a point object along the optical axis and recording the corresponding point spread holograms (PSHs). An object is mounted within the calibrated object space, and the object hologram was recorded under identical experimental conditions used for recording the PSHs. The image of the object at different depths was reconstructed by a cross-correlation between the object hologram and the PSHs. Application potential including bio-medical optics is discussed.
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Ben-Gurion University of the Negev1, University of Illinois at Chicago2, Swinburne University of Technology3, Brno University of Technology4, Chungbuk National University5, Beijing Institute of Technology6, Teikyo University of Science7, University of Georgia8, National Institute of Information and Communications Technology9, Tohoku University10, Tongji University11, Chinese Academy of Sciences12, Northern Arizona University13, Georgia Institute of Technology14, Wakayama University15
TL;DR: The current state-of-the-art FinCH technology, challenges, and future perspectives of FINCH technology as recognized by a diverse group of researchers contributing to different facets of research in FINCH have been presented in this paper.
Abstract: Fresnel incoherent correlation holography (FINCH) was a milestone in incoherent holography. In this roadmap, two pathways, namely the development of FINCH and applications of FINCH explored by many prominent research groups, are discussed. The current state-of-the-art FINCH technology, challenges, and future perspectives of FINCH technology as recognized by a diverse group of researchers contributing to different facets of research in FINCH have been presented.
35 citations
TL;DR: In this article, a phase-shifting interferometry technique using only two in-line phase-shift self-interference holograms is proposed, which is demonstrated by experiments on incoherent digital holographic microscopy and color-multiplexed fluorescence digital holography with computational coherent superposition.
Abstract: We propose a phase-shifting interferometry technique using only two in-line phase-shifted self-interference holograms. There is no requirement for additional recording or estimation in the measurement. The proposed technique adopts a mathematical model for self-interference digital holography. The effectiveness of the proposed technique is demonstrated by experiments on incoherent digital holographic microscopy and color-multiplexed fluorescence digital holography with computational coherent superposition. Two-color-multiplexed four-step phase-shifting incoherent digital holography is realized for the first time, to the best of our knowledge, using the proposed technique.
21 citations
TL;DR: This tutorial discusses one of the basic optical configurations of a lensless QPI technique based on the phase-retrieval algorithm andmented codes in Octave for image acquisition and automation using a web camera in an open source operating system are provided.
Abstract: Quantitative phase imaging (QPI) techniques are widely used for the label-free examining of transparent biological samples. QPI techniques can be broadly classified into interference-based and interferenceless methods. The interferometric methods which record the complex amplitude are usually bulky with many optical components and use coherent illumination. The interferenceless approaches which need only the intensity distribution and works using phase retrieval algorithms have gained attention as they require lesser resources, cost, space and can work with incoherent illumination. With rapid developments in computational optical techniques and deep learning, QPI has reached new levels of applications. In this tutorial, we discuss one of the basic optical configurations of a lensless QPI technique based on the phase-retrieval algorithm. Simulative studies on QPI of thin, thick, and greyscale phase objects with assistive pseudo-codes and computational codes in Octave is provided. Binary phase samples with positive and negative resist profiles were fabricated using lithography, and a single plane and two plane phase objects were constructed. Light diffracted from a point object is modulated by phase samples and the corresponding intensity patterns are recorded. The phase retrieval approach is applied for 2D and 3D phase reconstructions. Commented codes in Octave for image acquisition and automation using a web camera in an open source operating system are provided.
18 citations
TL;DR: In this paper, a modified approach to implement FINCH by a single camera shot by pre-calibrating the system involving recording of the point spread function library and reconstruction by a nonlinear cross correlation has been introduced.
Abstract: Fresnel incoherent correlation holography (FINCH) is a well-established incoherent imaging technique. In FINCH, three self-interference holograms are recorded with calculated phase differences between the two interfering, differently modulated object waves and projected into a complex hologram. The object is reconstructed without the twin image and bias terms by a numerical Fresnel back propagation of the complex hologram. A modified approach to implement FINCH by a single camera shot by pre-calibrating the system involving recording of the point spread function library and reconstruction by a non-linear cross correlation has been introduced recently. The expression of the imaging characteristics from the modulation functions in original FINCH and the modified approach by pre-calibration in spatial and polarization multiplexing schemes are reviewed. The study reveals that a reconstructing function completely independent of the function of the phase mask is required for the faithful expression of the characteristics of the modulating function in image reconstruction. In the polarization multiplexing method by non-linear cross correlation, a partial expression was observed, while in the spatial multiplexing method by non-linear cross correlation, the imaging characteristics converged towards a uniform behavior.
14 citations
TL;DR: In this paper, a denoising deep learning neural network (DLNN) was trained using synthetic noisy images generated by the convolution of recorded point spread functions with the virtual object functions under a wide range of aberrations and noises.
Abstract: Coded aperture imaging (CAI) technology is a rapidly evolving indirect imaging method with extraordinary potential. In recent years, CAI based on chaotic optical waves have been shown to exhibit multidimensional, multispectral, and multimodal imaging capabilities with a signal to noise ratio approaching the range of lens based direct imagers. However, most of the earlier studies used only narrow band illumination. In this study, CAI based on chaotic optical waves is investigated for white light illumination. A numerical study was carried out using scalar diffraction formulation and correlation optics and the lateral and axial resolving power for different spectral width were compared. A binary diffractive quasi-random lens was fabricated using electron beam lithography and the lateral and axial point spread holograms are recorded for white light. Three-dimensional imaging was demonstrated using thick objects consisting of two planes. An integrated sequence of signal processing tools such as non-linear filter, low-pass filter, median filter and correlation filter were applied to reconstruct images with an improved signal to noise ratio. A denoising deep learning neural network (DLNN) was trained using synthetic noisy images generated by the convolution of recorded point spread functions with the virtual object functions under a wide range of aberrations and noises. The trained DLNN was found to reduce further the reconstruction noises.
14 citations
References
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TL;DR: In this article, a structural similarity index is proposed for image quality assessment based on the degradation of structural information, which can be applied to both subjective ratings and objective methods on a database of images compressed with JPEG and JPEG2000.
Abstract: Objective methods for assessing perceptual image quality traditionally attempted to quantify the visibility of errors (differences) between a distorted image and a reference image using a variety of known properties of the human visual system. Under the assumption that human visual perception is highly adapted for extracting structural information from a scene, we introduce an alternative complementary framework for quality assessment based on the degradation of structural information. As a specific example of this concept, we develop a structural similarity index and demonstrate its promise through a set of intuitive examples, as well as comparison to both subjective ratings and state-of-the-art objective methods on a database of images compressed with JPEG and JPEG2000. A MATLAB implementation of the proposed algorithm is available online at http://www.cns.nyu.edu//spl sim/lcv/ssim/.
40,609 citations
TL;DR: Mature opto-electrical/mechanical technologies have enabled laser processing speeds approaching meters-per-second, leading to a fast lab-to-fab transfer and emerging biomedical applications implementing micrometer feature precision over centimeter-scale scaffolds and photonic wire bonding in telecommunications are highlighted.
Abstract: Processing of materials by ultrashort laser pulses has evolved significantly over the last decade and is starting to reveal its scientific, technological and industrial potential. In ultrafast laser manufacturing, optical energy of tightly focused femtosecond or picosecond laser pulses can be delivered to precisely defined positions in the bulk of materials via two-/multi-photon excitation on a timescale much faster than thermal energy exchange between photoexcited electrons and lattice ions. Control of photo-ionization and thermal processes with the highest precision, inducing local photomodification in sub-100-nm-sized regions has been achieved. State-of-the-art ultrashort laser processing techniques exploit high 0.1–1 μm spatial resolution and almost unrestricted three-dimensional structuring capability. Adjustable pulse duration, spatiotemporal chirp, phase front tilt and polarization allow control of photomodification via uniquely wide parameter space. Mature opto-electrical/mechanical technologies have enabled laser processing speeds approaching meters-per-second, leading to a fast lab-to-fab transfer. The key aspects and latest achievements are reviewed with an emphasis on the fundamental relation between spatial resolution and total fabrication throughput. Emerging biomedical applications implementing micrometer feature precision over centimeter-scale scaffolds and photonic wire bonding in telecommunications are highlighted.
835 citations
TL;DR: A new method for recording digital holograms under incoherent illumination, which results in a complex-valued Fresnel hologram that is reconstructed in the computer when the 3D properties of the object are revealed.
Abstract: We present a new method for recording digital holograms under incoherent illumination. Light is reflected from a 3D object, propagates through a diffractive optical element (DOE), and is recorded by a digital camera. Three holograms are recorded sequentially, each for a different phase factor of the DOE. The three holograms are superposed in the computer, such that the result is a complex-valued Fresnel hologram. When this hologram is reconstructed in the computer, the 3D properties of the object are revealed.
397 citations
TL;DR: In this paper, a motionless microscopy system (FINCHSCOPE) based on Fresnel incoherent correlation holography is presented for high-resolution 3D fluorescent images of biological speciments without the need for any moving parts.
Abstract: Holography is an attractive imaging technique as it offers the ability to view a complete three-dimensional volume from one image. However, holography is not widely applied to the field of three-dimensional fluorescence microscopic imaging, because fluorescence is incoherent and creating holograms requires a coherent interferometer system. Although scanning one beam of an interferometer pattern across the rear aperture of an objective to excite fluorescence in a specimen overcomes the coherence limitation, the mechanical scanning is complicated, which makes the image capturing slow, and the process is limited to low-numerical-aperture objectives. Here we present the first demonstration of a motionless microscopy system (FINCHSCOPE) based on Fresnel incoherent correlation holography, and its use in recording high-resolution three-dimensional fluorescent images of biological specimens. By using high-numerical-aperture objectives, a spatial light modulator, a CCD camera and some simple filters, FINCHSCOPE enables the acquisition of three-dimensional microscopic images without the need for scanning. Demonstration of an imaging system that can capture high-resolution 3D fluorescent images of biological speciments without the need for any moving parts.
369 citations
TL;DR: This work further analyzed FINCH in view of linear system theory and in comparison to conventional coherent and incoherent two dimensional imaging systems, and demonstrates improved resolution by FINCH, when compared to conventional imaging.
Abstract: Fresnel Incoherent Correlation Holography (FINCH) enables holograms to be recorded from incoherent light with just a digital camera and spatial light modulator. We previously described its application to general three dimensional incoherent imaging and specifically to fluorescence microscopy, wherein one complex hologram contains the three dimensional information in the field of view, obviating the need for scanning or serial sectioning. We have now further analyzed FINCH in view of linear system theory and in comparison to conventional coherent and incoherent two dimensional imaging systems. We demonstrate, theoretically and experimentally, improved resolution by FINCH, when compared to conventional imaging.
142 citations