Frizzled BRET sensors based on bioorthogonal labeling of unnatural amino acids reveal WNT-induced dynamics of the cysteine-rich domain.
Maria Kowalski-Jahn,Hannes Schihada,Ainoleena Turku,Thomas Huber,Thomas P. Sakmar,Thomas P. Sakmar,Gunnar Schulte +6 more
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In this paper, the role of WNT/FZD signaling in FZD1-10 signaling was investigated in the context of Wingless/Int-1 lipoglycoproteins (WNTs).Abstract:
Frizzleds (FZD1–10) are G protein–coupled receptors containing an extracellular cysteine-rich domain (CRD) binding Wingless/Int-1 lipoglycoproteins (WNTs). Despite the role of WNT/FZD signaling in ...read more
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Elucidating the Interactome of G Protein-Coupled Receptors and Receptor Activity-Modifying Proteins
TL;DR: A review of recent advances in techniques that allow the discovery of GPCR-RAMP interactions and their functional consequences and highlight prospects for future advances can be found in this paper , where the authors also provide an up-to-date list of reported G protein-coupled receptor (GPCR)-RAMP interaction based on a review of the current literature.
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Genetic code expansion to enable site‐specific bioorthogonal labeling of functional G protein‐coupled receptors in live cells
TL;DR: In this paper , a luciferase-based reporter assay was developed for site-specific bioorthogonal labeling of expressed G protein-coupled receptors (GPCRs) in live cells.
Journal ArticleDOI
WNT stimulation induces dynamic conformational changes in the Frizzled-Dishevelled interaction
Carl-Fredrik Bowin,Paweł Kozielewicz,Lukas Grätz,Maria Kowalski-Jahn,Hannes Schihada,Gunnar Schulte +5 more
TL;DR: In this article , ligand-dependent conformational changes in bioluminescence resonance energy transfer (BRET) between FZD5 and DVL2 were found to influence whether downstream signaling proceeds through DVL or G proteins.
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Reversible control of tetrazine bioorthogonal reactivity by naphthotube-mediated host-guest recognition
TL;DR: Hartung et al. as mentioned in this paper identified a high-affinity host-guest pair between synthetic macrocyclic naphthotubes and phenyltetrazine and developed a molecular-recognition strategy for controlling tetrazine reactions by host caging.
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Detecting and measuring of GPCR signaling – comparison of human induced pluripotent stem cells and immortal cell lines
Gaoxian Chen,Detlef Obal +1 more
TL;DR: In this article , a review summarizes existing resonance energy transfer and protein complementation assay technologies, as well as existing and new labeling methods for detecting GPCRs in human induced pluripotent stem cells (hiPSCs).
References
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