Journal ArticleDOI
From molecules to management: adopting DNA-based methods for monitoring biological invasions in aquatic environments.
John A. Darling,Andrew R. Mahon +1 more
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TLDR
The goal is to provide a useful assessment of the obstacles associated with integrating DNA-based methods into aquatic invasive species management, and to offer recommendations for future efforts aimed at overcoming those obstacles.About:
This article is published in Environmental Research.The article was published on 2011-10-01. It has received 408 citations till now.read more
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Journal ArticleDOI
Environmental DNA - An emerging tool in conservation for monitoring past and present biodiversity
TL;DR: The achievements gained through analyses of eDNA from macro-organisms in a conservation context are reviewed, its potential advantages and limitations are discussed, and it is expected the eDNA-based approaches to move from single-marker analyses of species or communities to meta-genomic surveys of entire ecosystems to predict spatial and temporal biodiversity patterns.
Journal ArticleDOI
Environmental DNA for wildlife biology and biodiversity monitoring
Kristine Bohmann,Kristine Bohmann,Alice Evans,M. Thomas P. Gilbert,M. Thomas P. Gilbert,Gary R. Carvalho,Simon Creer,Michael Knapp,Douglas W. Yu,Douglas W. Yu,Mark de Bruyn +10 more
TL;DR: In this paper, the authors examine the current frontiers of eDNA, outline key aspects requiring improvement, and suggest future developments and innovations for research, including improved ability to explore ecosystem-level processes, the generation of quantitative indices for analyses of species, community diversity, and dynamics, and novel opportunities through the use of time-serial samples and unprecedented sensitivity for detecting rare or difficult-to-sample taxa.
Environmental DNA for wildlife biology and biodiversity monitoring (vol 29, pg 358, 2014)
TL;DR: The current frontiers of eDNA are examined, key aspects requiring improvement are outlined, and future developments and innovations for research are suggested.
Journal ArticleDOI
Next-generation monitoring of aquatic biodiversity using environmental DNA metabarcoding
Alice Valentini,Pierre Taberlet,Pierre Taberlet,Claude Miaud,Raphaël Civade,Jelger Herder,Philip Francis Thomsen,Eva Bellemain,Aurélien Besnard,Eric Coissac,Eric Coissac,Frédéric Boyer,Frédéric Boyer,Coline Gaboriaud,Pauline Jean,Nicolas Poulet,Nicolas Roset,Gordon H. Copp,Gordon H. Copp,Philippe Geniez,Didier Pont,Christine Argillier,Jean-Marc Baudoin,Tiphaine Peroux,Alain J. Crivelli,Anthony Olivier,Manon Acqueberge,Matthieu Le Brun,Peter Rask Møller,Eske Willerslev,Tony Dejean +30 more
TL;DR: For amphibians, the detection probability with eDNA metabarcoding was 0.97 (CI = 0.90-0.99) vs. 0.58 (CI=0.50-0.63) for traditional surveys as mentioned in this paper.
Journal ArticleDOI
REVIEW: The detection of aquatic animal species using environmental DNA – a review of eDNA as a survey tool in ecology
TL;DR: New ideas for how eDNA can be used for conservation and management are included, for instance, the potential for on-site analyses, including the application of eDNA analysis to carbon nanotube platforms or laser transmission spectroscopy to facilitate rapid on- site detections.
References
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Journal ArticleDOI
Biological identifications through DNA barcodes
TL;DR: It is established that the mitochondrial gene cytochrome c oxidase I (COI) can serve as the core of a global bioidentification system for animals and will provide a reliable, cost–effective and accessible solution to the current problem of species identification.
Journal ArticleDOI
Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species
TL;DR: It is indicated that sequence divergences at COI regularly enable the discrimination of closely allied species in all animal phyla except the Cnidaria and constraints on intraspecific mitochondrial DNA divergence arising through selective sweeps mediated via interactions with the nuclear genome.
Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
TL;DR: The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.
Book ChapterDOI
Biological Identifications Through DNA Barcodes
TL;DR: It was established previously that the mitochondrial gene cytochrome c oxidase I can serve as the core of a global bioidentification system for animals and a new tools were developed recently to be complementary markers for (COI) DNA barcoding.