Functional significance of responsiveness to capacitating conditions in boar spermatozoa.
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...In these destabilized cells, when plasma membrane modifications and the increase in intracellular calcium occurred too rapidly, sperm capacitation soon followed; therefore, sperm membranes degenerated before fusion with the oocyte [13]....
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Cites methods from "Functional significance of responsi..."
...The samples incubated under capacitating conditions in Tyrode’s medium were then measured by flow cytometry as recently described (19)....
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...trol settings were adjusted for measuring the fluorescence in FL-1 and FL-3 channels essentially as described previously (19,20)....
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...reaction, and has been chosen from previously published studies (7,11,13,19)....
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55 citations
Cites background from "Functional significance of responsi..."
...For example, less than 60% of membrane- and acrosome-intact cells are often found after 2 h of incubation of fresh semen under capacitating conditions [11]....
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References
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"Functional significance of responsi..." refers background in this paper
...In mouse sperm plasma membrane, these changes are initiated by the massive cholesterol efflux mediated by BSA [2]....
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306 citations
"Functional significance of responsi..." refers background in this paper
...There have been reports of individual cases of variations in the response to bicarbonate as expressed by changes in merocyanine binding and motility or in cAMP levels between samples from different males [6,8,23,31]....
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...Consequently, further changes in binding sites occur to enable sperm to undergo stimuli-induced acrosome reaction [5–7]....
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298 citations
"Functional significance of responsi..." refers background in this paper
...However, the strong response to calcium ionophore A23187 may also be used as a monitoring system to identify sperm capacitation and acrosome reaction in individual males in different species [11–15]....
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