Journal ArticleDOI
Functional significance of responsiveness to capacitating conditions in boar spermatozoa.
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TLDR
The combination of sperm responsiveness parameters applied here is a suitable tool for the evaluation of sperm function and improved the prediction level of multiple regression models for farrowing rate and litter size.About:
This article is published in Theriogenology.The article was published on 2005-11-01. It has received 45 citations till now. The article focuses on the topics: Sperm & Capacitation.read more
Citations
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Journal ArticleDOI
Zinc alpha 2-glycoprotein: a multidisciplinary protein.
TL;DR: The discussion of the major aspects of ZAG from its gene structure to function and metabolism is discussed, which suggests that ZAG may have a role in the expression of the immune response.
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In vitro evaluation of sperm quality.
E. Mocé,James K. Graham +1 more
TL;DR: Current and past data, correlating laboratory assay data with sperm fertility are presented in an effort to determine which types of assays are important to conduct and when to conduct them.
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Identifying non-sperm particles during flow cytometric physiological assessment a simple approach
TL;DR: This work has developed a simple and cost-efficient flow cytometric approach for identifying non-sperm particles that can be carried out in parallel with functional assessments and is suitable for obtaining accurate sperm counts during routine semen evaluation.
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Freezability prediction of boar ejaculates assessed by functional sperm parameters and sperm proteins.
TL;DR: Assessing both the conventional sperm quality parameters and the immunolabeling of three proteins involved in the physiology of the sperm cell suggest that analyzing these sperm kinematic parameters could be a useful tool for predicting the potential freezability of an ejaculate.
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Identifying useable semen
TL;DR: Improved techniques for evaluating semen characteristics directly related to the fertilization process, such as IVM-IVF assays, analysis of seminal plasma protein markers, and discriminatory tests of sperm motility and morphology are reviewed with the goal of identifying high-index boars whose fertility is sustained when low numbers of sperm are used for AI.
References
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Capacitation mechanisms, and the role of capacitation as seen in eutherian mammals.
TL;DR: The findings suggest that capacitation is a series of positive destabilizing events that eventually lead to cell death, and that a succession of spermatozoa in the correct physiological state may be provided for the freshly ovulated egg.
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Sperm-Oviduct Interaction: Induction of Capacitation and Preferential Binding of Uncapacitated Spermatozoa to Oviductal Epithelial Cells in Porcine Species
TL;DR: The approach was to vary the proportions of uncapacitated, capacitated, or acrosome-reacted boar spermatozoa in suspension using long preincubation and lysophosphatidylcholine treatment of semen prior to a very short incubation with OEC or LLC-PK1 cells.
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Flow cytometric studies of bicarbonate‐mediated Ca2+ influx in boar sperm populations
TL;DR: Boar spermatozoa loaded with the Ca2+ probe fluo‐3 were incubated in various Tyrode's‐based media similar to those used for in vitro fertilization (IVF), and samples were analysed by two‐colour flow cytometry to detect membrane‐damaged (“dead”) cells.
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Artificial Insemination of Swine: Fecundity of Boar Semen Stored in Beltsville TS (BTS), Modified Modena (MM), or MR‐A and Inseminated on One, Three and Four Days After Collection12
TL;DR: BTS and MR-A are effective diluents for extension and storage of boar semen for use within the same week of collection and can give fertility equal to first day insemination if the sperm per insemination dose is doubled.
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The importance of porcine sperm parameters on fertility in vivo.
TL;DR: Sperm motility is a useful indicator of sperm fertilization capacity in vivo and it is critical that the number of spermatozoa used during insemination is sufficiently low to detect differences in sperm fertilizing efficiency.