Journal ArticleDOI
Functional significance of responsiveness to capacitating conditions in boar spermatozoa.
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TLDR
The combination of sperm responsiveness parameters applied here is a suitable tool for the evaluation of sperm function and improved the prediction level of multiple regression models for farrowing rate and litter size.About:
This article is published in Theriogenology.The article was published on 2005-11-01. It has received 45 citations till now. The article focuses on the topics: Sperm & Capacitation.read more
Citations
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Journal ArticleDOI
Zinc alpha 2-glycoprotein: a multidisciplinary protein.
TL;DR: The discussion of the major aspects of ZAG from its gene structure to function and metabolism is discussed, which suggests that ZAG may have a role in the expression of the immune response.
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In vitro evaluation of sperm quality.
E. Mocé,James K. Graham +1 more
TL;DR: Current and past data, correlating laboratory assay data with sperm fertility are presented in an effort to determine which types of assays are important to conduct and when to conduct them.
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Identifying non-sperm particles during flow cytometric physiological assessment a simple approach
TL;DR: This work has developed a simple and cost-efficient flow cytometric approach for identifying non-sperm particles that can be carried out in parallel with functional assessments and is suitable for obtaining accurate sperm counts during routine semen evaluation.
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Freezability prediction of boar ejaculates assessed by functional sperm parameters and sperm proteins.
TL;DR: Assessing both the conventional sperm quality parameters and the immunolabeling of three proteins involved in the physiology of the sperm cell suggest that analyzing these sperm kinematic parameters could be a useful tool for predicting the potential freezability of an ejaculate.
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Identifying useable semen
TL;DR: Improved techniques for evaluating semen characteristics directly related to the fertilization process, such as IVM-IVF assays, analysis of seminal plasma protein markers, and discriminatory tests of sperm motility and morphology are reviewed with the goal of identifying high-index boars whose fertility is sustained when low numbers of sperm are used for AI.
References
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Journal ArticleDOI
Cyclic AMP signalling during mammalian sperm capacitation--still largely terra incognita.
TL;DR: While cyclic AMP levels respond very rapidly to sperm treatment by effectors such as bicarbonate and Ca2+ (key components of capacitating media), there are big discrepancies between the rates of functional response.
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Enhanced Binding of Zona Pellucida Proteins to the Acrosomal Region of Intact Boar Spermatozoa in Response to Fertilizing Conditions: A Flow Cytometric Study'
TL;DR: It is suggested that the observed changes in zona binding ability bring about optimal sperm-egg attachment; they may also relate to induction of the acrosome reaction by zona pellucida components.
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Capacitation and acrosome reaction in buffalo bull spermatozoa assessed by chlortetracycline and Pisum sativum agglutinin fluorescence assay.
TL;DR: Two methods for detecting the acrosomal status of buffalo spermatozoa are described, namely chlortetracycline fluorescence assay and Pisum sativum agglutinin (FITC-PSA) stain and they detect an increase or decrease in physiological acrosome reactions.
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Regulation of capacitation of canine spermatozoa during co-culture with heterologous oviductal epithelial cells
TL;DR: It can be concluded that tyrosine phosphorylation of head membrane proteins and capacitation are delayed in canine spermatozoa being in closed contact with oviductal epithelium.
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Quality control in boar semen production by use of the FACSCount AF system
TL;DR: The second experiment found that variation in the number of motile sperm per insemination dose could be reduced significantly if the FACSCount AF flow cytometer rather than the Corning 254 spectrophotometer was used for determination of sperm concentration in the raw semen.