Journal ArticleDOI
Functionalization of 2′-amino-LNA with additional nucleobases
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TLDR
Oligonucleotides containing these modified nucleotides show in most cases increased thermal stability when forming duplexes with complementary DNA, even allowing multiple incorporations.Abstract:
Thymin-1-yl-acetic acid and adenin-9-yl-acetic acid have been coupled to the N2′-atom of a 2′-amino-LNA thymine nucleoside, and these “double-headed” LNA monomers have been incorporated into oligodeoxyribonucleotides via their corresponding phosphoramidite derivatives. Oligonucleotides containing these modified nucleotides show in most cases increased thermal stability when forming duplexes with complementary DNA, even allowing multiple incorporations. Incorporation of “double-headed” LNA monomers in both strands also led to stable duplexes, however, no indication of Watson–Crick base pairing between the extra nucleobases could be found.read more
Citations
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Book ChapterDOI
Biological activity and biotechnological aspects of locked nucleic acids.
Karin E. Lundin,Torben Højland,Bo Hansen,Robert Persson,Jesper B. Bramsen,Jørgen Kjems,Troels Koch,Jesper Wengel,C. I. Edvard Smith +8 more
TL;DR: The use of LNA in antisense ONs, including gapmers, splice-switching Ons, and siLNA, as well as antigene ONS, is reviewed and new LNA modifications and the adaptation of enzymes for LNA incorporation are reviewed.
Journal ArticleDOI
Amino acids attached to 2′-amino-LNA: synthesis and excellent duplex stability
TL;DR: The synthesis of amino acid functionalised 2'-amino-LNA derivatives is described, and the modified ONs induce a marked increase in thermal stability, which is particularly apparent in a buffer system with a low salt concentration.
Journal ArticleDOI
The synthesis of double-headed nucleosides by the CuAAC reaction and their effect in secondary nucleic acid structures.
Anna Søndergaard Jørgensen,Khalil Isak Shaikh,Gerald Enderlin,Elise Ivarsen,Surender Kumar,Poul Nielsen +5 more
TL;DR: Both thymine and adenine in the 2'-position were found to stabilise three-way junctions in both dsDNA and DNA : RNA contexts and to give cross-strand interactions in a DNA-duplex, when specifically introduced in a so-called (+1)-zipper motif.
Journal ArticleDOI
Three pyrene-modified nucleotides: synthesis and effects in secondary nucleic acid structures.
TL;DR: All three pyrene nucleotides lead to thermal stabilization of bulged duplexes and three-way junctions and can act as specific recognition probes in oligonucleotides when two pyrenes were introduced into the core of these complexes.
Journal ArticleDOI
Additional Base‐Pair Formation in DNA Duplexes by a Double‐Headed Nucleotide
Charlotte Madsen,Sarah Witzke,Pawan Kumar,Kushuma Negi,Pawan K. Sharma,Michael B. Petersen,Poul Nielsen +6 more
TL;DR: A double-headed nucleotide is designed and synthesised that presents two nucleobases in the interior of a dsDNA duplex that recognises and forms Watson-Crick base pairs with two complementary adenosines in a Watson- Crick framework and may form the basis for a complete series of double- headed nucleotides based on all 16 base combinations of the four natural nucleobase combinations.
References
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Journal ArticleDOI
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Journal ArticleDOI
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Journal ArticleDOI
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