Journal ArticleDOI
Gas-liquid chromatography of protein amino acid trimethylsilyl derivatives.
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TLDR
The gas-liquid chromatographic separation of the N-trimethylsilyl TMS esters of the twenty protein amino acids was achieved after evaluation of a number of combinations of siloxane liquid phases and reaction conditions were investigated for the quantitative silylation.About:
This article is published in Journal of Chromatography A.The article was published on 1969-01-01. It has received 119 citations till now. The article focuses on the topics: Derivatization & Trimethylsilyl.read more
Citations
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Development and Performance of a Gas Chromatography−Time-of-Flight Mass Spectrometry Analysis for Large-Scale Nontargeted Metabolomic Studies of Human Serum
Paul Begley,Sue Francis-McIntyre,Warwick B. Dunn,David Broadhurst,Antony Halsall,Andy Tseng,Joshua Knowles,Royston Goodacre,Douglas B. Kell +8 more
TL;DR: A method for the preparation and GC-TOF-MS analysis of human serum samples has been developed and evaluated for application in long-term metabolomic studies and showed the ability to define metabolite differences between samples from a population and samples spiked with metabolites standards.
Journal ArticleDOI
Trimethylsilylation of amino acids derivatization and chromatography
TL;DR: The data for amino acids in ribonuclease, β-casein, K- casein, soybean meal, and blood are in good agreement with the values obtained by the classical ion-exchange method, and establishes the use of the trimethylsilyl/gas—liquid chromatographic method for the quantitative analysis of biological materials.
Journal ArticleDOI
Gas chromatography of amino acids.
Petr Hušek,Karel Macek +1 more
TL;DR: This review summarizes all papers that have appeared on the gas chromatography of amino acids (including the iodoamino acids) and their enantiomers in the period 1956-mid-1974.
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The polypeptide composition of bovine epidermal alpha-keratin.
P M Steinert,W W Idler +1 more
TL;DR: The polypedtide chains that comprise the subunits of the tonofilaments, or th alpha-keratin component, of bovine epidermis were fractionated by combination of chromatography on DEAE-cellulose and preparative polyacrylamide-gel electrophoresis on a molar basis.
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Gas-liquid chromatography of amino acids in biological substances.
TL;DR: The development of a gas—liquid chromatographic (GLC) method for the quantitative analysis of amino acids in complex biological substances, specifically blood plasma and urine, has been achieved and data obtained were in excellent agreement with results by classical ion exchange.
References
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Silylations with Bis(trimethylsilyl)acetamide, a Highly Reactive Silyl Donor
TL;DR: In this paper, the silylation of amides, ureas, and amino acids, as well as hindered phenols, carboxylic acids, and enols, is described.
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A new silylation reagent for amino acids bis (trimethylsilyl)trifluoroacetamide (BSTFA)
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Gas chromatography of sugars and other polyhydroxy compounds.
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Gas-liquid chromatographic analysis of nucleic acid components.
TL;DR: Application of the developed method to the analysis of biological materials was accomplished using perchloric acid hydrolysis and anion-exchange removal of the bases from biological background prior to derivatization and GLC analysis.