GATA3 Mediates a Fast, Irreversible Commitment to BMP4-Driven Differentiation in Human Embryonic Stem Cells
Alexandra Gunne-Braden,Adrienne E. Sullivan,Borzo Gharibi,Rahuman S. Malik Sheriff,Alok Kumar Maity,Yi-Fang Wang,Amelia Edwards,Ming Jiang,Michael Howell,Robert J. Goldstone,Roy Wollman,Philip East,Silvia D.M. Santos +12 more
Reads0
Chats0
TLDR
It is proposed that early commitment may be a feature of BMP-driven fate choices and that interlinked feedback is the molecular basis for an irreversible transition from pluripotency to differentiation.About:
This article is published in Cell Stem Cell.The article was published on 2020-04-16 and is currently open access. It has received 46 citations till now. The article focuses on the topics: Embryonic stem cell.read more
Citations
More filters
Integrative Genomics Viewer
James T. Robinson,Helga Thorvaldsdottir,Wendy Winckler,Mitchell Guttman,Eric S. Lander,Eric S. Lander,Gad Getz,Jill P. Mesirov +7 more
TL;DR: The sheer volume and scope of data posed by this flood of data pose a significant challenge to the development of efficient and intuitive visualization tools able to scale to very large data sets and to flexibly integrate multiple data types, including clinical data.
Journal ArticleDOI
Synthetic multistability in mammalian cells
TL;DR: MultiFate as discussed by the authors uses engineered zinc finger transcription factors that transcriptionally self-activate as homodimers and mutually inhibit one another through heterodimerization to generate seven states, each stable for at least 18 days.
Journal ArticleDOI
GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program.
Yoji Kojima,Chika Yamashiro,Yusuke Murase,Yukihiro Yabuta,Ikuhiro Okamoto,Chizuru Iwatani,Hideaki Tsuchiya,Masataka Nakaya,Masataka Nakaya,Tomoyuki Tsukiyama,Tomoyuki Tsukiyama,Tomonori Nakamura,Takuya Yamamoto,Mitinori Saitou +13 more
TL;DR: In this paper, the authors explored transcription factors (TFs) that engender the germ-cell fate in their pluripotent precursors and provided a paradigm for TF-based human gametogenesis.
Journal ArticleDOI
Translating Embryogenesis to Generate Organoids: Novel Approaches to Personalized Medicine.
Sounak Sahu,Shyam K. Sharan +1 more
TL;DR: Insight from developmental biology is provided with a comprehensive resource of signaling pathways that in a coordinated manner form embryo-like structures and organoids that hold great promise for drug discovery, clinical management, and personalized medicine.
Journal ArticleDOI
NLRP7 plays a functional role in regulating BMP4 signaling during differentiation of patient-derived trophoblasts
TL;DR: The human iPSC model of a genetic placental disease recapitulates aspects of trophoblast biology, highlights the broad utility of iPSCs-derived trophoblasts for modeling human placental diseases and identifies NLRP7 as an essential modulator of key developmental cell fate regulators.
References
More filters
Journal ArticleDOI
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2
TL;DR: This work presents DESeq2, a method for differential analysis of count data, using shrinkage estimation for dispersions and fold changes to improve stability and interpretability of estimates, which enables a more quantitative analysis focused on the strength rather than the mere presence of differential expression.
Journal ArticleDOI
Fast and accurate short read alignment with Burrows–Wheeler transform
Heng Li,Richard Durbin +1 more
TL;DR: Burrows-Wheeler Alignment tool (BWA) is implemented, a new read alignment package that is based on backward search with Burrows–Wheeler Transform (BWT), to efficiently align short sequencing reads against a large reference sequence such as the human genome, allowing mismatches and gaps.
Journal ArticleDOI
Fiji: an open-source platform for biological-image analysis
Johannes Schindelin,Ignacio Arganda-Carreras,Erwin Frise,Verena Kaynig,Mark Longair,Tobias Pietzsch,Stephan Preibisch,Curtis Rueden,Stephan Saalfeld,Benjamin Schmid,Jean-Yves Tinevez,Daniel J. White,Volker Hartenstein,Kevin W. Eliceiri,Pavel Tomancak,Albert Cardona +15 more
TL;DR: Fiji is a distribution of the popular open-source software ImageJ focused on biological-image analysis that facilitates the transformation of new algorithms into ImageJ plugins that can be shared with end users through an integrated update system.
Journal ArticleDOI
Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles
Aravind Subramanian,Pablo Tamayo,Vamsi K. Mootha,Sayan Mukherjee,Benjamin L. Ebert,Michael A. Gillette,Amanda G. Paulovich,Scott L. Pomeroy,Todd R. Golub,Eric S. Lander,Jill P. Mesirov +10 more
TL;DR: The Gene Set Enrichment Analysis (GSEA) method as discussed by the authors focuses on gene sets, that is, groups of genes that share common biological function, chromosomal location, or regulation.
Journal ArticleDOI
Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources.
TL;DR: By following this protocol, investigators are able to gain an in-depth understanding of the biological themes in lists of genes that are enriched in genome-scale studies.
Related Papers (5)
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2
Chromatin-state barriers enforce an irreversible mammalian cell fate decision.
M. Andrés Blanco,M. Andrés Blanco,M. Andrés Blanco,David B. Sykes,Lei Gu,Mengjun Wu,Mengjun Wu,Ricardo Petroni,Rahul Karnik,Rahul Karnik,Mathias Wawer,Joshua Rico,Haitao Li,William D. Jacobus,William D. Jacobus,Ashwini Jambhekar,Ashwini Jambhekar,Sihem Cheloufi,Alexander Meissner,Konrad Hochedlinger,David T. Scadden,Yang Shi,Yang Shi +22 more