Gene Genealogies and AFLP Analyses in the Fusarium oxysporum Complex Identify Monophyletic and Nonmonophyletic Formae Speciales Causing Wilt and Rot Disease
R. P. Baayen,Kerry O'Donnell,Peter J. M. Bonants,Elizabeth Cigelnik,Laurens P. N. M. Kroon,Eugène J. A. Roebroeck,Cees Waalwijk +6 more
Reads0
Chats0
TLDR
Strains in vegetative compatibility groups (VCGs) shared identical sequences and AFLP profiles, supporting the monophyly of the two single-VCG formae speciales, lilii and tulipae, and Parsimony analyses of AFLP fingerprint data supported the gene Genealogy-based phylogram; however, AFLP-based phylogenies were considerably more homoplasious than the gene genealogies.Abstract:
The monophyletic origin of host-specific taxa in the plant-pathogenic Fusarium oxysporum complex was tested by constructing nuclear and mitochondrial gene genealogies and amplified fragment length polymorphism (AFLP)-based phylogenies for 89 strains representing the known genetic and pathogenic diversity in 8 formae speciales associated with wilt diseases and root and bulb rot. We included strains from clonal lineages of F. oxysporum f. spp. asparagi, dianthi, gladioli, lilii, lini, opuntiarum, spinaciae, and tulipae. Putatively nonpathogenic strains from carnation and lily were included and a reference strain from each of the three main clades identified previously in the F. oxysporum complex; sequences from related species were used as outgroups. DNA sequences from the nuclear translation elongation factor 1alpha and the mitochondrial small subunit (mtSSU) ribosomal RNA genes were combined for phylogenetic analysis. Strains in vegetative compatibility groups (VCGs) shared identical sequences and AFLP profiles, supporting the monophyly of the two single-VCG formae speciales, lilii and tulipae. Identical genotypes were also found for the three VCGs in F. oxysporum f. sp. spinaciae. In contrast, multiple evolutionary origins were apparent for F. oxysporum f. spp. asparagi, dianthi, gladioli, lini, and opuntiarum, although different VCGs within each of these formae speciales often clustered close together or shared identical EF-1alpha and mtSSU rDNA haplotypes. Kishino-Hasegawa analyses of constraints forcing the monophyly of these formae speciales supported the exclusive origin of F. oxysporum f. sp. opuntiarum but not the monophyly of F. oxysporum f. spp. asparagi, dianthi, gladioli, and lini. Most of the putatively nonpathogenic strains from carnation and lily, representing unique VCGs, were unrelated to F. oxysporum f. spp. dianthi and lilii, respectively. Putatively nonpathogenic or rot-inducing strains did not form exclusive groups within the molecular phylogeny. Parsimony analyses of AFLP fingerprint data supported the gene genealogy-based phylogram; however, AFLP-based phylogenies were considerably more homoplasious than the gene genealogies. The predictive value of the forma specialis naming system within the F. oxysporum complex is questioned.read more
Citations
More filters
Journal ArticleDOI
The magnitude of fungal diversity: the 1.5 million species estimate revisited
TL;DR: The number of known species of fungi is estimated as at least 74 K, but could be as much as 120 K with allowances for ‘orphaned’ species as discussed by the authors, which is the current working hypothesis for the number of fungi on Earth.
Journal ArticleDOI
Pathogen profile update: Fusarium oxysporum
TL;DR: More than 120 different formae speciales have been identified based on specificity to host species belonging to a wide range of plant families as mentioned in this paper, which can cause severe losses in many vegetables and flowers, field crops, such as cotton, and plantation crops such as banana, date palm and oil palm.
Journal ArticleDOI
Phytophthora ramorum sp. nov., a new pathogen on Rhododendron and Viburnum
Sabine Werres,Rainer Marwitz,Willem A. Man In't veld,Arthur W. A. M. de Cock,Peter J. M. Bonants,Marjanne de Weerdt,Karin Themann,E. Ilieva,R. P. Baayen +8 more
TL;DR: It was concluded that a new Phytophthora species, described here as P. ramorum sp.
Journal ArticleDOI
Fusarium oxysporum and its biocontrol
TL;DR: Results showed the great diversity affecting the soil-borne populations of F. oxysporum, with several modes of action contributing to their biocontrol capacity.
Journal ArticleDOI
Fusarium Wilt of Banana
TL;DR: An overview of the Panama disease and its causal agent, Fusarium oxysporum f. cubense, is presented in this paper, with an emphasis on tropical race 4 (TR4), a 'Cavendish'-killing variant of the pathogen that has spread dramatically in the Eastern Hemisphere.
References
More filters
Journal ArticleDOI
AFLP: a new technique for DNA fingerprinting.
Pieter Vos,René Cornelis Josephus Hogers,Marjo Bleeker,Martin Reijans,Theo van de Lee,Miranda Hornes,Adrie Friters,Jerina Pot,Johan Paleman,Martin Kuiper,Marc Zabeau +10 more
TL;DR: The AFLP technique provides a novel and very powerful DNA fingerprinting technique for DNAs of any origin or complexity that allows the specific co-amplification of high numbers of restriction fragments.
Journal ArticleDOI
Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous
TL;DR: The results suggest that the ancestral ITS2 types may have arisen following an ancient interspecific hybridization or gene duplication which occurred prior to the evolutionary radiation of the Gibberella fujikuroi complex and related species of Fusarium.
Journal ArticleDOI
Multiple evolutionary origins of the fungus causing Panama disease of banana: Concordant evidence from nuclear and mitochondrial gene genealogies
TL;DR: Testing whether lineages of the Panama disease pathogen have a monophyletic origin by comparing DNA sequences of nuclear and mitochondrial genes indicates Panama disease of banana is caused by fungi with independent evolutionary origins.
Journal ArticleDOI
Molecular systematics and phylogeography of the Gibberella fujikuroi species complex.
TL;DR: Phylogenetic relationships of the phyto-pathogenic Gibberella fujikuroi species complex were investigated by maximum parsimony analysis of DNA sequences from multiple loci.
Related Papers (5)
Comparative genomics reveals mobile pathogenicity chromosomes in Fusarium
Li-Jun Ma,H. Charlotte van der Does,Katherine A. Borkovich,Jeffrey J. Coleman,Marie Josée Daboussi,Antonio Di Pietro,Marie Dufresne,Michael Freitag,Manfred Grabherr,Bernard Henrissat,Petra M. Houterman,Seogchan Kang,Won-Bo Shim,Charles P. Woloshuk,Xiaohui Xie,Jin-Rong Xu,John F. Antoniw,Scott E. Baker,B. H. Bluhm,Andrew Breakspear,Daren W. Brown,Robert A. E. Butchko,Sinéad B. Chapman,Richard M.R. Coulson,Pedro M. Coutinho,Etienne Danchin,Etienne Danchin,Andrew C. Diener,Liane R. Gale,Donald M. Gardiner,Stephen A. Goff,Kim E. Hammond-Kosack,Karen Hilburn,Aurélie Hua-Van,Wilfried Jonkers,Kemal Kazan,Chinnappa D. Kodira,Michael Koehrsen,Lokesh Kumar,Yong-Hwan Lee,Liande Li,Liande Li,John M. Manners,Diego Miranda-Saavedra,Mala Mukherjee,Gyungsoon Park,Jongsun Park,Sook Young Park,Sook Young Park,Robert H. Proctor,Aviv Regev,M. Carmen Ruiz-Roldán,Divya Sain,Sharadha Sakthikumar,Sean M. Sykes,David C. Schwartz,B. Gillian Turgeon,Ilan Wapinski,Olen C. Yoder,Sarah Young,Qiandong Zeng,Shiguo Zhou,James E. Galagan,Christina A. Cuomo,H. Corby Kistler,Martijn Rep +65 more